Biomacromolecules,
Год журнала:
2024,
Номер
25(11), С. 6967 - 6986
Опубликована: Окт. 17, 2024
Supramolecular
peptide
hydrogels
(SPHs)
consist
of
peptides
containing
hydrogelators
and
functional
epitopes,
which
can
first
self-assemble
into
nanofibers
then
physically
entangle
together
to
form
dynamic
three-dimensional
networks.
Their
porous
structures,
excellent
bioactivity,
high
dynamicity,
similar
an
extracellular
matrix
(ECM),
have
great
potential
in
artificial
ECM.
The
properties
the
hydrogel
are
largely
dependent
on
peptides.
noncovalent
interactions
among
drive
formation
assemblies
further
transition
hydrogels,
while
bioactive
epitopes
modulate
cell-cell
cell-ECM
interactions.
Therefore,
SPHs
support
cell
growth,
making
them
ideal
biomaterials
for
ECM
mimics.
This
Review
outlines
classical
molecular
design
from
summarizes
recent
advancements
as
ECMs
nervous
system
repair,
wound
healing,
bone
cartilage
regeneration,
organoid
culture.
emerging
SPH
platform
could
provide
alternative
strategy
developing
more
effective
tissue
engineering.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Дек. 23, 2024
Islet
transplantation
is
a
promising
therapy
for
diabetes,
yet
the
limited
survival
and
functionality
of
transplanted
islet
grafts
hinder
optimal
outcomes.
Glucagon-like
peptide-1
(GLP-1),
an
endogenous
hormone,
has
shown
potential
to
enhance
function;
however,
its
systemic
administration
can
result
in
poor
localization
undesirable
side
effects.
To
address
these
challenges,
we
developed
novel
peptide-based
nanofiber
hydrogel
incorporating
GLP-1
localized
delivery.
By
conjugating
FFG
tripeptide
(a
self-assembling
motif
derived
from
phenylalanine-phenylalanine-glycine)
C-terminus
native
GLP-1,
engineered
GLP-1-FFG,
peptide
that
forms
robust
structure
resistant
enzymatic
degradation.
When
GLP-1-FFG
co-assembles
with
biotin-DFYIGSRGD
(referred
as
SupraGel),
supramolecular
polypeptide
previously
identified
containing
motifs
extracellular
matrix
components,
resulting
(SupraGel
+
GLP-1-FFG)
creates
stable
nanofibrous
network
excellent
rheological
properties.
In
vitro,
this
significantly
improves
function
survival.
Bulk
RNA
sequencing
results
demonstrate
suppresses
expression
hypoxia-related
genes,
downregulates
pro-inflammatory
upregulates
genes
associated
function.
Further
analysis
reveals
effects
are
related
activation
AKT
signaling
pathway.
syngeneic
mouse
model,
application
SupraGel
at
renal
subcapsular
transplant
site
enhanced
efficacy
marginal-dose
transplantation,
by
improved
glycemic
control,
faster
higher
rates
diabetes
reversal,
better
glucose
tolerance,
greater
graft
diabetic
recipient
mice.
This
innovative
nanotechnology-based
offers
strategy
enhancing
therapy.
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Biphenyl-capped
phosphopeptides
instruct
cell
aggregation
into
spheroids,
with
minimal
effective
concentrations
below
10
μM.
Key
factors
driving
morphogenesis
include
the
self-assembly
ability
and
dynamic
shapeshifting
of
peptide
assemblies.
Biomacromolecules,
Год журнала:
2024,
Номер
25(11), С. 6967 - 6986
Опубликована: Окт. 17, 2024
Supramolecular
peptide
hydrogels
(SPHs)
consist
of
peptides
containing
hydrogelators
and
functional
epitopes,
which
can
first
self-assemble
into
nanofibers
then
physically
entangle
together
to
form
dynamic
three-dimensional
networks.
Their
porous
structures,
excellent
bioactivity,
high
dynamicity,
similar
an
extracellular
matrix
(ECM),
have
great
potential
in
artificial
ECM.
The
properties
the
hydrogel
are
largely
dependent
on
peptides.
noncovalent
interactions
among
drive
formation
assemblies
further
transition
hydrogels,
while
bioactive
epitopes
modulate
cell-cell
cell-ECM
interactions.
Therefore,
SPHs
support
cell
growth,
making
them
ideal
biomaterials
for
ECM
mimics.
This
Review
outlines
classical
molecular
design
from
summarizes
recent
advancements
as
ECMs
nervous
system
repair,
wound
healing,
bone
cartilage
regeneration,
organoid
culture.
emerging
SPH
platform
could
provide
alternative
strategy
developing
more
effective
tissue
engineering.
