Supramolecular Coassembly Activated Dual-Excitation Fluorescent Sensing Platform for Precise Detection of Aflatoxin B₁

Analytical Chemistry, Год журнала: 2025, Номер 97(2), С. 1357 - 1365

Опубликована: Янв. 7, 2025

The development of a sensory signal amplification approach is very crucial for rapid and precise detection aflatoxin B1 (AFB1). However, such approaches remain scarce due to the weak interactions between AFB1 sensing probes. Herein, first example dual-excitation fluorescent platform antibody-free reported, which assembled by an ordered π–π stack cationic perylene derivative (PTHA) tris(2,2′-bipyridine)ruthenium(II) [Ru(bpy3)2+]. Taking advantage stepwise assembly multiple binding sites nanoprobe, its ability capturing significantly improved driven noncovalent interactions. Interestingly, mode with superposition self-calibration activated in supramolecular coassembly process. Under excitation 365 nm 440 nm, exhibits specific recognition toward limit determined be 0.12 ng mL–1. Notably, demonstrates exceptional sensitivity enhancements 106-fold, revealing that self-calibrated reference improves accuracy analytical method significantly. applications our not only crack problem via strategy but also provide universal sensitization ultrasensitive analysis complex environments.

Язык: Английский

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Debut of enzyme-responsive anionic cyanine for overlap-free NIR-II-to-I dual-channel tumour imaging

Chemical Science, Год журнала: 2025, Номер 16(10), С. 4490 - 4500

Опубликована: Янв. 1, 2025

Bridging the disparity between traditional surgical resection imaging and ex vivo histopathology, fluorescence is considered a promising tool in disease diagnosis navigation. Nevertheless, its usefulness undermined by variability of single-wavelength signals limited penetration NIR-I (650-900 nm) bioimaging. In this work, we present novel NIR-II ratiometric fluorescent probe (CFC-GSH) with γ-glutamyl transpeptidase (GGT) sensitivity for multifunctional This leverages GSH-capped anionic cyanine, advantages high brightness, excellent photostability, specificity favourable biocompatibility. CFC-GSH exhibits an intrinsically stable signal prior to triggering, which can be utilized systemic circulation vessel outlining microvascular imaging. At tumour site GGT over expression, intramolecular S,N-rearrangement would initiate conversion sulphur-substituted cyanine amino-substituted resulting significant emission shift 270 nm. Using dual-channel changes, effectively differentiates subcutaneous hepatocellular carcinoma (HCC) normal tissue precisely localizes metastatic HCC tumours abdominal cavity. These results reveal that potential as multiprospective candidate screening diagnostic various biological scenarios.

Язык: Английский

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NIR‐II Fluorescence/Photoacoustic Dual Ratiometric Probes with Unique Recognition Site for Quantitatively Visualizing H2S2 in Vivo

Angewandte Chemie International Edition, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 13, 2024

Hydrogen persulfide (H

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NIR‐II Orthogonal Fluorescent Ratiometric Nanoprobe for In Situ Bioimaging of Carbon Monoxide

Small, Год журнала: 2024, Номер unknown

Опубликована: Сен. 20, 2024

Abstract Carbon monoxide (CO) functions as a significant endogenous cell signaling molecule and is strongly associated with many physiological pathological processes. However, conventional fluorescence imaging in the visible near‐infrared (NIR) I regions suffers autofluorescence background photon scattering, hindering accurate detection of CO vivo. In addition, complexity environments leads to fluctuating emission. To solve these problems, herein, NIR‐II fluorescent nanoprobe NP‐Pd for vivo ratiometric bioimaging developed. presence CO, exhibits responsive enhancement absorption at 808 nm, which amplifies signal down‐conversion nanoparticles (DCNP) 1060 nm under excitation, while DCNP 1525 980 excitation remains unchanged serves an internal standard. Through this orthogonally strategy, precise diagnosis acute liver injury diseases are achieved mouse model experiments, providing novel tool CO‐related diseases.

Язык: Английский

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NIR‐II Fluorescence/Photoacoustic Dual Ratiometric Probes with Unique Recognition Site for Quantitatively Visualizing H2S2 in Vivo

Angewandte Chemie, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 13, 2024

Abstract Hydrogen persulfide (H 2 S ) plays a significant role in redox biology and signal transduction; therefore, quantitative visualization of H the deep tissue living organisms is essential for obtaining reliable information about relevant pathophysiological processes directly. However, currently reported probes are unsuitable this purpose because their poor selectivity many polysulfide species or short wavelength, which hinders precise imaging tissues. Herein, first time, we report unique ‐mediated dithiole formation reaction. Based on reaction, construct NIR‐II fluorescence (FL) photoacoustic (PA) dual‐ratiometric probe ( NIR‐II‐H quantitatively visualizing vivo. This shows (I 840 /I 1000 , 107‐fold) (PA 800 /PA 900 6.5‐fold) responses towards Na with high specificity, excellent sensitivity (1.8 nM), improved water solubility, deep‐tissue penetration. More importantly, using FL/PA imaging, successfully demonstrated that could be used to accurately quantify fluctuating levels liver‐injury mouse models induced by lipopolysaccharides metformin drugs. Overall, study not only presents promising tool ‐related pathological research, but also provides recognition site may generalized designing more useful agents future.

Язык: Английский

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