Current Opinion in Electrochemistry, Год журнала: 2024, Номер 49, С. 101627 - 101627
Опубликована: Дек. 5, 2024
Язык: Английский
Sensors & Diagnostics, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Herein, we review the recent developments and applications of ECL for immunosensing in different areas, such as detection biomarkers, bead-based bacteria cell analysis, provide future perspectives new developments.
Язык: Английский
Процитировано
6
ACS Sensors, Год журнала: 2025, Номер unknown
Опубликована: Янв. 23, 2025
It is crucial yet challenging to sensitively quantify low-abundance biomarkers in blood for early screening and diagnosis of various diseases. Herein, an analytical model intra-mesopore immunoassay (IMIA) was proposed, which competent examine at the femtomolar level. The success rooted design innovative superparamagnetic core-shell structure with Fe3O4 nanoparticles (NPs) core hierarchically porous zeolitic imidazolate frameworks as a shell (Fe3O4@HPZIF-8), achieved through soft-template directed self-assembly coupled confinement growth mechanism. Such unique configuration conceptualized IMIA where HPZIF-8 served solid carrier cover capture antibodies while assisted its rapid separation. large pore channels not only provided stable microenvironment maintain recognition ability captured but also enhanced their coating density, thus promoting probability capturing binding target antigens, significantly improving (IA) sensitivity. practical clinic IA cTnI (Cardiac Troponin I, biomarker acute myocardial infarction (AMI)) human serums exemplified. developed could accurately slight fluctuations concentrations AMI patients different stages after symptom onset more than 100-fold enhancement limit detection (LOD) comparison conventional plate-based enzyme-linked immunosorbent assay (ELISA). high sensitivity makes it powerful tool accurate diseases by altering type primary antibody.
Язык: Английский
Процитировано
0
Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160553 - 160553
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
ACS Applied Polymer Materials, Год журнала: 2025, Номер unknown
Опубликована: Март 10, 2025
Colorimetric lateral flow assays (LFAs) are widely used for detecting analytes through color changes displayed by conjugates on a test strip, typically made of materials such as nitrocellulose (NC) membranes. However, significant number conjugate nanoparticles become trapped within the membrane's porous structure, preventing full display information. Here, gelation NC membrane is achieved introducing dimethyl sulfoxide (DMSO) into an LFA resulting in structural and high transparency membrane, exposing red-colored Au transparent portable DMSO/NC membrane. Under optimized conditions (16 μL 50 vol % DMSO water heating strip at 80 °C 8 min), becomes highly transparent, achieving 77.42% transmittance while maintaining its capability to effectively perform LFAs cardiac troponin I (cTnI) both buffer solutions clinical samples. We anticipate that this method holds promise advancing fundamental studies has potential applications healthcare environmental monitoring.
Язык: Английский
Процитировано
0
Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137602 - 137602
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Talanta, Год журнала: 2025, Номер unknown, С. 128159 - 128159
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Current Opinion in Electrochemistry, Год журнала: 2024, Номер 49, С. 101627 - 101627
Опубликована: Дек. 5, 2024
Язык: Английский
Процитировано
1