Flexible Microelectrode Arrays Based on Vacuum Filling for Electrophysiology Sensing of Cardiomyocytes DOI

Wangzihan Zhang,

Shanshan You,

Yike Zhou

и другие.

ACS Applied Electronic Materials, Год журнала: 2024, Номер 6(9), С. 6431 - 6439

Опубликована: Авг. 21, 2024

Microelectrode arrays (MEAs) are widely used platforms for the electrophysiological monitoring of cardiomyocytes, providing an effective method to enhance efficiency drug development and analyze disease models. However, traditional MEAs fabricated on rigid silicon or glass substrates, which causes a huge mismatch modulus with myocardial tissue. In this work, we proposed vacuum filling technology bismuth–indium (Bi–In) alloy flexible MEA fabrication. The was realized at elevated temperature inside polydimethylsiloxane microchannels when Bi–In in its liquid state. Then, then underwent phase transition form solid-state electrodes returned room temperature. showed good flexibility, could resist bending (180°), had electrochemical impedance 174.3 kΩ 1 kHz electrode diameter is 50 μm. devices were demonstrated recording signals from human-induced pluripotent stem cell-derived presented excellent biocompatibility, able detect typical field potential waveforms amplitudes >900 μV. This work presents approach advancement fabrication serves as robust tool application preclinical analysis.

Язык: Английский

Soft and Flexible Bioelectronic Micro‐Systems for Electronically Controlled Drug Delivery DOI Creative Commons
Massimo Mariello, Ismail Eş, Christopher M. Proctor

и другие.

Advanced Healthcare Materials, Год журнала: 2023, Номер unknown

Опубликована: Ноя. 4, 2023

The concept of targeted and controlled drug delivery, which directs treatment to precise anatomical sites, offers benefits such as fewer side effects, reduced toxicity, optimized dosages, quicker responses. However, challenges remain engineer dependable systems materials that can modulate host tissue interactions overcome biological barriers. To stay aligned with advancements in healthcare precision medicine, novel approaches are imperative improve effectiveness, biocompatibility, compliance. Electronically delivery (ECDD) has recently emerged a promising approach calibrated spatial temporal precision. This article covers recent breakthroughs soft, flexible, adaptable bioelectronic micro-systems designed for ECDD. It overviews the most widely reported operational modes, engineering strategies, electronic interfaces, characterization techniques associated ECDD systems. Further, it delves into pivotal applications wearable, ingestible, implantable medical devices. Finally, discourse extends future prospects

Язык: Английский

Процитировано

16

Printed Silk Microelectrode Arrays for Electrophysiological Recording and Controlled Drug Delivery DOI Creative Commons
Nouran Adly, Tetsuhiko Teshima, Hossein Hassani

и другие.

Advanced Healthcare Materials, Год журнала: 2023, Номер 12(17)

Опубликована: Фев. 24, 2023

The use of soft and flexible bioelectronic interfaces can enhance the quality for recording cells' electrical activity by ensuring a continuous intimate contact with smooth, curving surfaces found in physiological environment. This work develops microelectrode arrays (MEAs) made silk fibroin (SF) films that also serve as drug delivery system. Inkjet printing is used tool to deposit substrate, conductive electrode, insulator, well drug-delivery nanocomposite film. approach highly versatile, shown fabrication carbon microelectrodes, sandwiched between substrate insulator. technique permits development thin-film devices be employed vitro extracellular recordings HL-1 cell action potentials. tuning SF applying an stimulus produce permeable layer on-demand systems demonstrated. multifunctional MEA developed here pave way screening time-resolved localized chemical stimuli.

Язык: Английский

Процитировано

10

All Biodisintegratable Hydrogel Biohybrid Neural Interfaces with Synergistic Performances of Microelectrode Array Technologies, Tissue Scaffolding, and Cell Therapy DOI Open Access

Wan‐Lou Lei,

Chih‐Wei Peng, S. C. Chiu

и другие.

Advanced Functional Materials, Год журнала: 2023, Номер 34(3)

Опубликована: Окт. 12, 2023

Abstract Biohybrid neural interfaces (BHNIs) are a new class of neuromodulating devices that integrate microelectrode arrays (MEAs) and cell transplantation to improve treatment nerve injuries disorders. However, current BHNI made from abiotic materials usually bio‐passive, non‐biodisintegratable, or rigid, which restricts encapsulated activity host reconstruction frequently leads local tissue inflammation. Herein, the first MEA composed all disintegratable hydrogel scaffold with synergistic performances conformal adhesiveness, technologies, scaffolding stem therapy on time scale appropriate for repair is proposed. In particular, conductive tracks extracellular matrix (ECM)‐based double‐cross‐linked dual‐electrically (ECH) systems robust tissue‐mimicking chemical/physical properties, electrical conductivity, an affinity progenitor cells. Meanwhile, substrate prepared transglutaminase‐incorporated gelatin/silk precursors simultaneously promotes gelation interfacial adhesion between stacks, leading rapid scalable device integration. When full subjected various mechanical stimuli moisture, it structurally stable low impedance (4 ± 3 kΩ) comparable recently reported benchmark. With seamless lamination around peripheral fibers, permits successive signal monitoring wound condition evaluation, while demonstrating effects spatiotemporally controlled stimulation accelerate restoration motor function. This completely degraded by 1 month thus eliminating need surgical retrieval stably remain, interact, further fuse tissues, successfully exhibiting compatible integration biology implanted system.

Язык: Английский

Процитировано

10

Bioprinting Soft 3D Models of Hematopoiesis using Natural Silk Fibroin‐Based Bioink Efficiently Supports Platelet Differentiation DOI Creative Commons
Christian A. Di Buduo, Marco Lunghi, Volodymyr Kuzmenko

и другие.

Advanced Science, Год журнала: 2024, Номер 11(18)

Опубликована: Март 21, 2024

Abstract Hematopoietic stem and progenitor cells (HSPCs) continuously generate platelets throughout one's life. Inherited Platelet Disorders affect ≈ 3 million individuals worldwide are characterized by defects in platelet formation or function. A critical challenge the identification of these diseases lies absence models that facilitate study hematopoiesis ex vivo. Here, a silk fibroin‐based bioink is developed designed for 3D bioprinting. This replicates soft biomimetic environment, enabling controlled differentiation HSPCs into platelets. The formulation consisting fibroin, gelatin, alginate fine‐tuned to obtain viscoelastic, shear‐thinning, thixotropic with remarkable ability rapidly recover after bioprinting provide structural integrity mechanical stability over long‐term culture. Optical transparency allowed high‐resolution imaging generation, while incorporation enzymatic sensors quantitative analysis glycolytic metabolism during represented through measurable color changes. Bioprinting patient samples revealed decrease metabolic activity production Disorders. These discoveries instrumental establishing reference ranges classification automating assessment treatment responses. model has far‐reaching implications application research blood‐related diseases, prioritizing drug development strategies, tailoring personalized therapies.

Язык: Английский

Процитировано

4

Designing Silk Biomaterials toward Better Future Healthcare: The Development and Application of Silk‐Based Implantable Electronic Devices in Clinical Diagnosis and Therapy DOI Creative Commons
Qiying Lv, Qilin Li, Peng Cao

и другие.

Advanced Materials, Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Implantable medical electronic devices (IMEDs) have attracted great attention and shown versatility for solving clinical problems ranging from real-time monitoring of physiological/ pathological states to electrical stimulation therapy brain cell activity deep stimulation. The ongoing challenge is select appropriate materials in target device configuration biomedical applications. Currently, silk-based biomaterials been developed the design diagnostic therapeutic due their excellent properties abundant active sites structure. Herein, aim summarize structural characteristics, physicochemical properties, bioactivities natural silk as well derived materials, with a particular focus on implantable devices, such invasive brain-computer interfaces, neural recording, vivo electrostimulation. In addition, future opportunities challenges are also envisioned, hoping spark interests researchers interdisciplinary fields biomaterials, medicine, electronics.

Язык: Английский

Процитировано

4

An Implantable and Degradable Silk Sericin Protein Film Energy Harvester for Next‐Generation Cardiovascular Electronic Devices DOI Open Access
Qiying Lv, Shiyu Chen, Dan Luo

и другие.

Advanced Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 2, 2025

Abstract Current cardiovascular implantable electronic devices (CIEDs) face a pressing clinical need for the development of battery‐free, biodegradable, and biocompatible to mitigate risk adverse in vivo responses. To address this demand, it is proposed utilizing natural biomaterial, silk sericin (SS), which exhibits valuable biological activities contains abundant asymmetric amino acids with adjustable structures, create an self‐powered system based on piezoelectric principle. The functionalized SS‐based (F‐SS‐based) film demonstrates high longitudinal tensor ( d 33 ) 12 pC N −1 . An energy‐generating device (EG device) can generate electric energy under mechanical force both vitro vivo. By manually tapping EG‐device few minutes, accumulated electricity commercial capacitor (1.1 µF) could illuminate LEDs or operate timer. Furthermore, instantaneous power density (218.5 µW m −2 achieved by manual EG sufficient deliver effective pacing restart non‐beating heart normalize atrioventricular block preclinical model. Owing its biocompatibility biodegradability physiological environments, F‐SS‐based holds significant promise advancement systems next‐generation CIEDs other degradable devices.

Язык: Английский

Процитировано

0

Electro- and mechano-responsive vesicular carriers targeting tumors DOI

Omar Khan,

Sumedh Bahadure,

Sri Pada Datta Chigurupati

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 283 - 315

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Printed Electronic Devices and Systems for Interfacing with Single Cells up to Organoids DOI Creative Commons
M. Saghafi, Srivatsan K. Vasantham, Navid Hussain

и другие.

Advanced Functional Materials, Год журнала: 2023, Номер 34(20)

Опубликована: Дек. 13, 2023

Abstract The field of bioelectronics with the aim to contact cells, cell clusters, biological tissues and organoids has become a vast enterprise. Currently, it is mainly relying on classical micro‐ nanofabrication methods build devices systems. Very recently highly pushed by development novel printable organic, inorganic biomaterials as well advanced digital printing technologies such laser inkjet employed in this endeavor. Recent advantages alternative additive manufacturing 3D enable interesting new routes, particular for applications requiring incorporation delicate or creation scaffold structures that show high potential building hybrid bio‐/inorganic devices. Here current state printed 2D electronic related lithography techniques interfacing systems are reviewed. focus lies vitro single cell, organoids. Challenges future prospects discussed all‐printed bio/electronic targeting biomedical research, diagnostics, health monitoring.

Язык: Английский

Процитировано

7

Wetting Behavior of Inkjet-Printed Electronic Inks on Patterned Substrates DOI Creative Commons
Pooja Arya, Yanchen Wu, Fei Wang

и другие.

Langmuir, Год журнала: 2024, Номер 40(10), С. 5162 - 5173

Опубликована: Фев. 26, 2024

In inkjet printing technology, one important factor influencing the quality and reliability of printed films is interaction jetted ink with substrate surface. This short-range determines wettability adhesion to solid surface hence responsible for final shape deposited ink. Here, we investigate wetting morphologies inkjet-printed inks on patterned substrates by carefully designed experimental test structures simulations. The contact angles, properties, drop shapes, as well their influence device variability, are experimentally theoretically analyzed. For simulations, employ phase-field method, which based free energy minimization two-phase system given boundary conditions. Through a systematic investigation drops consisting hydrophilic hydrophobic areas, report that morphology related not only layout volume but also strategy wettability. Furthermore, show how can modify intrinsic enhance reliability. Based present findings, cast light improvement fabrication thin film transistors.

Язык: Английский

Процитировано

2

Flexible Microelectrode Arrays Based on Vacuum Filling for Electrophysiology Sensing of Cardiomyocytes DOI

Wangzihan Zhang,

Shanshan You,

Yike Zhou

и другие.

ACS Applied Electronic Materials, Год журнала: 2024, Номер 6(9), С. 6431 - 6439

Опубликована: Авг. 21, 2024

Microelectrode arrays (MEAs) are widely used platforms for the electrophysiological monitoring of cardiomyocytes, providing an effective method to enhance efficiency drug development and analyze disease models. However, traditional MEAs fabricated on rigid silicon or glass substrates, which causes a huge mismatch modulus with myocardial tissue. In this work, we proposed vacuum filling technology bismuth–indium (Bi–In) alloy flexible MEA fabrication. The was realized at elevated temperature inside polydimethylsiloxane microchannels when Bi–In in its liquid state. Then, then underwent phase transition form solid-state electrodes returned room temperature. showed good flexibility, could resist bending (180°), had electrochemical impedance 174.3 kΩ 1 kHz electrode diameter is 50 μm. devices were demonstrated recording signals from human-induced pluripotent stem cell-derived presented excellent biocompatibility, able detect typical field potential waveforms amplitudes >900 μV. This work presents approach advancement fabrication serves as robust tool application preclinical analysis.

Язык: Английский

Процитировано

0