Pump‐Less, Recirculating Organ‐on‐Chip (rOoC) Platform to Model the Metabolic Crosstalk between Islets and Liver

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(13)

Опубликована: Янв. 15, 2024

Abstract Type 2 diabetes mellitus (T2DM), obesity, and metabolic dysfunction‐associated steatotic liver disease (MASLD) are epidemiologically correlated disorders with a worldwide growing prevalence. While the mechanisms leading to onset development of these conditions not fully understood, predictive tissue representations for studying coordinated interactions between central organs that regulate energy metabolism, particularly pancreatic islets, needed. Here, dual pump‐less recirculating organ‐on‐chip platform combines human pluripotent stem cell (sc)‐derived sc‐liver sc‐islet organoids is presented. The reproduces key aspects cross‐talk both organs, including glucose levels selected hormones, supports viability functionality while preserving reduced release pro‐inflammatory cytokines. In model disruption in response treatment high lipids fructose, exhibit hallmarks steatosis insulin resistance, sc‐islets produce cytokines on‐chip. Finally, known effects anti‐diabetic drugs Taken together, provides basis functional studies T2DM, MASLD on‐chip, as well testing potential therapeutic interventions.

Язык: Английский

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Modelling host–microbiome interactions in organ-on-a-chip platforms

Nature Reviews Bioengineering, Год журнала: 2023, Номер 2(2), С. 175 - 191

Опубликована: Ноя. 17, 2023

Язык: Английский

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Advances in the Generation of Constructed Cardiac Tissue Derived from Induced Pluripotent Stem Cells for Disease Modeling and Therapeutic Discovery

Cells, Год журнала: 2024, Номер 13(3), С. 250 - 250

Опубликована: Янв. 29, 2024

The human heart lacks significant regenerative capacity; thus, the solution to failure (HF) remains organ donation, requiring surgery and immunosuppression. demand for constructed cardiac tissues (CCTs) model treat disease continues grow. Recent advances in induced pluripotent stem cell (iPSC) manipulation, CRISPR gene editing, 3D tissue culture have enabled a boom iPSC-derived CCTs (iPSC-CCTs) with diverse types architecture. Compared 2D-cultured cells, iPSC-CCTs better recapitulate biology, demonstrating potential advance modeling, drug discovery, medicine, though could benefit from methods faithfully mimic physiology electrophysiology. Here, we summarize future developments vascularization, immunization, maturation of study therapy.

Язык: Английский

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Global Literature Analysis of Tumor Organoid and Tumor-on-Chip Research

Cancers, Год журнала: 2025, Номер 17(1), С. 108 - 108

Опубликована: Янв. 1, 2025

Background: Tumor organoid and tumor-on-chip (ToC) platforms replicate aspects of the anatomical physiological states tumors. They, therefore, serve as models for investigating tumor microenvironments, metastasis, immune interactions, especially precision drug testing. To map changing research diversity focus in this field, we performed a quality-controlled text analysis categorized academic publications clinical studies. Methods: Previously, collected metadata on organoids or organ-on-chip from PubMed, Web Science, Scopus, EMBASE, bioRxiv, published between January 2011 June 2023. Here, selected documents corpus that were computationally determined relevant to analyzed them using an in-house algorithm. Additionally, ClinicalTrials.gov studies related ToC March Results Discussion: From 3551 139 trials, identified 55 24 classes modeled models, respectively. The was particularly active neural hepatic/pancreatic organoids, well gastrointestinal, neural, reproductive models. Comparative with cancer statistics showed lung, lymphatic, cervical tumors under-represented research. Our findings also illustrate varied topics, including physiology, therapeutic approaches, cell involvement, analytical techniques. Mapping geographically highlighted colorectal Netherlands, though overall specific countries did not reflect regional prevalence. These insights only current landscape but indicate potential new directions model

Язык: Английский

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Organoid, organ-on-a-chip and traditional Chinese medicine

Chinese Medicine, Год журнала: 2025, Номер 20(1)

Опубликована: Фев. 12, 2025

Abstract In the past few years, emergence of organoids and organ-on-a-chip (OOAC) technologies, which are complementary to animal models two-dimensional cell culture methods can better simulate internal environment human body, provides a new platform for traditional Chinese medicine (TCM) studies. Organoids OOAC techniques have been increasingly applied in fields drug screening, assessment development, personalized therapies, developmental biology, there some application cases TCM this review, we summarized current status using organoid technologies research provide key insights future study. It is believed that will play more important roles make greater contributions innovative development TCM.

Язык: Английский

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A generic pump‐free organ‐on‐a‐chip platform for assessment of intestinal drug absorption

Biotechnology Journal, Год журнала: 2024, Номер 19(2)

Опубликована: Фев. 1, 2024

Abstract Organ‐on‐a‐chip technology has shown great potential in disease modeling and drug evaluation. However, traditional organ‐on‐a‐chip devices are mostly pump‐dependent with low throughput, which makes it difficult to leverage their advantages. In this study, we have developed a generic, pump‐free platform consisting of 32‐unit chip an adjustable rocker, facilitating high‐throughput dynamic cell culture straightforward operation. By utilizing the rocker induce periodic fluid forces, can achieve fluidic conditions similar those obtained pump‐based systems. Through constructing gut‐on‐a‐chip model, observed remarkable enhancements expression barrier‐associated proteins spatial distribution differentiated intestinal cells compared static culture. Furthermore, RNA sequencing analysis unveiled enriched pathways associated proliferation, lipid transport, metabolism, indicating ability mimic critical physiological processes. Additionally, tested seven drugs that represent range high, medium, vivo permeability using model found strong correlation between P app values human Fa, demonstrating capability for absorption Our findings highlight as valuable tool advancing development enabling personalized medicine.

Язык: Английский

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Complex in vitro models positioned for impact to drug testing in pharma: a review

Biofabrication, Год журнала: 2024, Номер 16(4), С. 042006 - 042006

Опубликована: Авг. 27, 2024

Abstract Recent years have seen the creation and popularization of various complex in vitro models (CIVMs), such as organoids organs-on-chip, a technology with potential to reduce animal usage pharma while also enhancing our ability create safe efficacious drugs for patients. Public awareness CIVMs has increased, part, due recent passage FDA Modernization Act 2.0. This visibility is expected spur deeper investment adoption models. Thus, end-users model developers alike require framework both understand readiness current enter drug development process, assess upcoming same. review presents selection based on comparative -omics data (which we term model-omics), metrics qualification specific test assays that may support context-of-use (COU) assays. We surveyed existing healthy tissue ten development-critical organs body, provide evaluations suggestions improving model-omics COU each. In whole, this comes from perspective, seeks an evaluation where are poised maximum impact roadmap realizing potential.

Язык: Английский

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Deep learning based characterization of human organoids using optical coherence tomography

Biomedical Optics Express, Год журнала: 2024, Номер 15(5), С. 3112 - 3112

Опубликована: Апрель 9, 2024

Organoids, derived from human induced pluripotent stem cells (hiPSCs), are intricate three-dimensional in vitro structures that mimic many key aspects of the complex morphology and functions vivo organs such as retina heart. Traditional histological methods, while crucial, often fall short analyzing these dynamic due to their inherently static destructive nature. In this study, we leveraged capabilities optical coherence tomography (OCT) for rapid, non-invasive imaging both retinal, cerebral, cardiac organoids. Complementing this, developed a sophisticated deep learning approach automatically segment organoid tissues internal structures, hollows chambers. Utilizing advanced analysis platform, quantitatively assessed critical parameters, including size, area, volume, beating, offering comprehensive live characterization classification These findings provide profound insights into differentiation developmental processes organoids, positioning quantitative OCT potentially transformative tool future research.

Язык: Английский

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Three-dimensional cardiac models: a pre-clinical testing platform

Biochemical Society Transactions, Год журнала: 2024, Номер 52(3), С. 1045 - 1059

Опубликована: Май 23, 2024

Major advancements in human pluripotent stem cell (hPSC) technology over recent years have yielded valuable tools for cardiovascular research. Multi-cell type 3-dimensional (3D) cardiac models particular, are providing complementary approaches to animal studies that better representatives than simple 2-dimensional (2D) cultures of differentiated hPSCs. These 3D can be broadly divided into two categories; namely those generated through aggregating pre-differentiated cells and form self-organizing structures during their vitro differentiation from either replicate aspects development or enable the examination interactions among constituent types, with some these showing increased maturity compared 2D systems. Both groups already emerged as physiologically relevant pre-clinical platforms studying heart disease mechanisms, exhibiting key functional attributes heart. In this review, we describe different organoid derived hPSCs, generation methods, applications research use drug screening. We also address current limitations challenges testing propose potential improvements enhance efficacy discovery.

Язык: Английский

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Establishment and characterization of turtle liver organoids provides a potential model to decode their unique adaptations

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Фев. 22, 2024

Abstract Painted turtles are remarkable for their freeze tolerance and supercooling ability along with associated resilience to hypoxia/anoxia oxidative stress, rendering them an ideal biomedical model hypoxia-induced injuries (including strokes), tissue cooling during surgeries, organ cryopreservation. Yet, such research is hindered by seasonal reproduction slow maturation. Here we developed characterized adult stem cell-derived turtle liver organoids (3D self-assembled in vitro structures) from painted, snapping, spiny softshell spanning ~175My of evolution, a subset cryopreserved. This development is, the best our knowledge, first this vertebrate Order, complements only other non-avian reptile snake venom glands. Preliminary characterization, including morphological, transcriptomic, proteomic analyses, revealed enriched cholangiocytes. Deriving distant life stages demonstrates that techniques broadly applicable chelonians, permitting functional genomic tools currently lacking herpetological research. Such platform could potentially support studies genome-to-phenome mapping, gene function, genome architecture, adaptive responses climate change, implications ecological, evolutionary,

Язык: Английский

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