ESIPT Nano‐Emitter to Probe Lysosome Biogenesis in Live Cells
Small,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 25, 2025
Endosome-lysosome
fusion
and
endo-lysosome
fission-mediated
lysosome
biogenesis
are
crucial
in
regulating
cellular
health,
their
dysregulation
signifies
disease.
Tracking
such
intricate
events
with
minimal
disturbance
remains
elusive
due
to
the
scarcity
of
single-component
synthetic
probes
capable
distinctly
simultaneously
labeling
both
endosomes
lysosomes.
Here,
an
amphiphilic
π-conjugated
imine
probe
is
designed
that
forms
micellar
self-assemblies
water,
called
Nano-emitter,
which
labels
lysosomes
upon
monochromatic-wavelength
excitation.
ESIPT
(Excited
State
Intramolecular
Proton
Transfer)
active
Nano-emitter
shows
red
fluorescence
at
endosomal
pH.
Its
hydrolysis
fluorescent
amine,
PEG-Naph
lysosomal
pH
illuminated
green,
amine
excitable
using
a
405
nm
confocal
laser.
The
two-color
enabled
tracking
lysosome-biogenesis
processes
living
cells.
Using
multiplexed
time-lapse
imaging
anti-cancer
drug
doxorubicin,
role
these
investigated
lysosome-mediated
doxorubicin
sequestration
MCF-7
results
show
as
well
endo-lysosomes
also
sequestered
apart
from
Interestingly,
doxorubicin-sequestered
underwent
fission
generated
more
lysosomes,
preventing
drug's
nuclear
localization.
Such
versatile
can
enhance
understanding
foster
therapeutic
strategies.
Язык: Английский
Applications of Matrix Metalloproteinase-9-Related Nanomedicines in Tumors and Vascular Diseases
Pharmaceutics,
Год журнала:
2025,
Номер
17(4), С. 479 - 479
Опубликована: Апрель 7, 2025
Matrix
metalloproteinase-9
(MMP-9)
is
implicated
in
tumor
progression
and
vascular
diseases,
contributing
to
angiogenesis,
metastasis,
extracellular
matrix
degradation.
This
review
comprehensively
examines
the
relationship
between
MMP-9
these
pathologies,
exploring
underlying
molecular
mechanisms
signaling
pathways
involved.
Specifically,
we
discuss
contribution
of
epithelial–mesenchymal
transition,
as
well
its
involvement
a
spectrum
including
macrovascular,
cerebrovascular,
ocular
diseases.
focuses
on
recent
advances
MMP-9-targeted
nanomedicine
strategies,
highlighting
design
application
responsive
nanoparticles
for
enhanced
drug
delivery.
These
nanotherapeutic
strategies
leverage
overexpression
achieve
targeted
release,
improved
penetration,
reduced
systemic
toxicity.
We
explore
various
nanoparticle
platforms,
such
liposomes
polymer
nanoparticles,
their
action,
degradation,
targeting
specificity.
Finally,
address
challenges
posed
by
heterogeneity
expression
implications
personalized
therapies.
Ultimately,
this
underscores
diagnostic
therapeutic
potential
nanomedicines
against
tumors
Язык: Английский
Star-Polymer Unimolecular Micelle Nanoparticle to Deliver Payload Across the Blood-Brain Barrier
Nanoscale,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Blood–Brain
Barrier
:
A
biodegradable
star
block
unimolecular
micellar
nanoplatform
was
designed
and
developed
to
breach
the
tightly
controlled
blood–brain
barrier
deliver
anticancer
drugs
brain
tissue-penetrable
NIR
biomarkers.
Язык: Английский
Amphiphilic block copolymers of poly(vinylidene fluoride) and poly(ethylene glycol)
Polymers for Advanced Technologies,
Год журнала:
2024,
Номер
35(6)
Опубликована: Май 30, 2024
Abstract
Amphiphilic
block
copolymers
of
poly(ethylene
glycol)
(PEG)
and
poly(vinylidene
fluoride)
(PVDF)
were
successfully
synthesized
by
growing
on
PEG‐chain
transfer
agent
(PEG‐macro‐CTA)
using
reversible
addition–fragmentation
chain
(RAFT)
polymerization.
MeO‐PEG‐XA,
XA‐PEG‐XA
macro‐CTAs
(XA
refers
to
the
xanthate
moiety),
esterification
MeO‐PEG‐OH
(20,000
g
mol
−1
)
HO‐PEG‐OH
with
2‐bromopropionyl
bromide
followed
reaction
potassium
ethyl
xanthate,
used
synthesize
MeO‐PEG‐
b
‐PVDF
PVDF‐
‐PEG‐
‐PVDF,
respectively.
The
characterized
NMR
(
1
H,
13
C
19
F),
FTIR,
SEC.
Furthermore,
PVDF/PEG
copolymer
formation
was
proved
H
DOSY
NMR.
thermal
properties
determined
TGA
DSC.
crystallinity
electroactive
phases
revealed
XRD
FTIR.
size
shape
self‐assembled
in
DMF/water
mixtures
DLS,
TEM
critical
micelle
concentration
fluorescence
spectroscopy.
influence
molecular
weight
PVDF
content
is
discussed.
Язык: Английский
Structural Engineering of Cationic Block Copolymer Architectures for Selective Breaching of Prokaryotic and Eukaryotic Biological Species
ACS Applied Bio Materials,
Год журнала:
2024,
Номер
7(11), С. 7062 - 7075
Опубликована: Окт. 18, 2024
Positively
charged
antimicrobial
polymers
are
known
to
cause
severe
damage
biological
systems,
and
thus
synthetic
strategies
urgently
required
design
next-generation
nontoxic
cationic
macromolecular
architectures
for
healthcare
applications.
Here,
we
report
a
structural-engineering
strategy
build
linear
star-block
copolymer
nanoarchitectures
having
identical
chemical
composition,
molar
mass,
nanoparticle
size,
positive
surface
charge,
yet
they
differ
distinctly
in
their
action
breaching
prokaryotic
species
such
as
E.
coli
(Gram-negative
bacteria)
without
affecting
eukaryotic
like
red-blood
mammalian
cells.
For
this
purpose,
structures
built
on
polycaprolactone
biodegradable
platform
an
imidazolium
handle.
Under
physiological
conditions,
the
architecture
exhibits
toxicity
indiscriminately
all
species,
whereas
its
star
counterpart
is
remarkably
selective
membrane
toward
bacteria
while
maintaining
inertness
species.
Confocal
microscopy
analysis
of
HPTS
fluorescent
dye-loaded
star-polymer
nanoparticles
substantiated
coli.
Tissue-penetrable
near-infrared
dye
(IR-780)
loaded
NP
aided
vivo
biodistribution
ex
quantification
species'
accumulations
vital
organs
mice.
Azithromycin,
clinical
water-insoluble
macrolide,
delivered
from
accomplish
synergistic
activity
by
combination
bactericidal–bacteriostatic
polymer
carrier
drug
together
single
system.
Язык: Английский