Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 6, 2024
Abstract
Bone
marrow
stem
cells
(BMSCs)‐engineered
cartilage
(BEC)
shows
promise
for
clinically
repairing
defects.
However,
when
implanted
in
immunocompetent
large
animals,
BEC
becomes
susceptible
to
ossification
due
inflammatory
infiltration.
To
address
this,
a
nanofilm
isolation
approach
is
developed
enhance
BEC's
chondrogenic
stability.
Tacrolimus
(FK506),
known
its
immunosuppressive
effect,
integrated
into
adipic
dihydrazide
(ADH)‐modified
hyaluronic
acid
(HA),
creating
an
acid‐responsive
macromolecular
prodrug
called
FK506@HA‐ADH.
This
then
blended
with
poly(lactic‐co‐glycolic
acid)
(PLGA)
form
electrospun
FK506@HA/PLGA
nanofilm.
Goat‐derived
BMSCs
are
induced
vitro
BEC,
which
enclosed
within
the
and
subcutaneously
autologous
goats.
The
acted
as
physical
barrier,
preventing
immunocyte
Additionally,
response
acidic
environment
triggered
by
inflammation
gradual
degradation
of
PLGA,
FK506@HA‐ADH
cleaved,
releasing
FK506
needed.
released
effectively
countered
cytokines
promoted
cartilaginous
maturity.
These
combined
mechanisms
significantly
inhibited
hypertrophy
improved
stability
goat
model.
nanofilm‐based
strategy
established
niche,
successfully
endochondral
promoting
stable
formation
BEC.
advancements
crucial
translating
cell‐based
therapies
clinical
use
repair.
Materials Today Bio,
Год журнала:
2025,
Номер
31, С. 101502 - 101502
Опубликована: Янв. 19, 2025
DNA-based
hydrogels
stand
out
for
bone
regeneration
due
to
their
exceptional
biocompatibility
and
programmability.
These
facilitate
the
formation
of
spatial
structures
through
bulk
hydrogel
fabricating,
microsphere
formatting,
3D
printing.
Furthermore,
microenvironment
can
be
finely
tuned
by
leveraging
degradation
products,
nanostructure,
targeting,
delivery
capabilities
inherent
materials.
In
this
review,
we
underscore
advantages
hydrogels,
detailing
composition,
gelation
techniques,
structure
optimization.
We
then
delineate
three
critical
elements
in
promotion
using
hydrogels:
(i)
osteogenesis
driven
phosphate
ions,
plasmids,
oligodeoxynucleotides
(ODNs)
that
enhance
mineralization
promote
gene
protein
expression;
(ii)
vascularization
facilitated
tetrahedral
DNA
nanostructures
(TDNs)
aptamers,
which
boosts
expression
targeted
release;
(iii)
immunomodulation
achieved
loaded
factors,
TDNs,
bound
ions
stimulate
macrophage
polarization
exhibit
antibacterial
properties.
With
these
properties,
used
construct
organoids,
providing
an
innovative
tool
disease
modeling
therapeutic
applications
tissue
engineering.
Finally,
discuss
current
challenges
future
prospects,
emphasizing
potential
impacts
regenerative
medicine.
Materials Today Bio,
Год журнала:
2025,
Номер
31, С. 101509 - 101509
Опубликована: Янв. 22, 2025
Articular
cartilage,
composed
of
chondrocytes
within
a
dynamic
viscoelastic
matrix,
has
limited
self-repair
capacity,
posing
significant
challenge
for
regeneration.
Constructing
high-fidelity
cartilage
organoids
through
three-dimensional
(3D)
bioprinting
to
replicate
the
structure
and
physiological
functions
is
crucial
regenerative
medicine,
drug
screening,
disease
modeling.
However,
commonly
used
matrix
bioinks
lack
reversible
cross-linking
precise
controllability,
hindering
cellular
regulation.
Thus,
encoding
adaptive
cultivating
an
attractive
idea.
DNA,
with
its
ability
be
intricately
encoded
reversibly
cross-linked
into
hydrogels,
offers
manipulation
at
both
molecular
spatial
structural
levels.
This
endows
hydrogels
viscoelasticity,
printability,
cell
recognition,
stimuli
responsiveness.
paper
elaborates
on
strategies
encode
bioink
via
emphasizing
regulation
predictable
properties
resulting
interactions
behavior.
The
significance
these
construction
highlighted.
Finally,
we
discuss
challenges
future
prospects
using
DNA-encoded
3D
bioprinted
organoids,
underscoring
their
potential
impact
advancing
biomedical
applications.
Regenerative Medicine,
Год журнала:
2025,
Номер
20(1), С. 45 - 55
Опубликована: Янв. 2, 2025
Articular
cartilage
lesion
frequently
leads
to
dysfunction
and
the
development
of
degenerative
diseases,
posing
a
significant
public
health
challenge
due
limited
self-healing
capacity
tissue.
Current
surgical
treatments,
including
marrow
stimulation
techniques
osteochondral
autografts/allografts,
have
efficacy
or
drawbacks,
highlighting
urgent
need
for
alternative
strategies.
Advances
in
3D
printing
regeneration
shown
promising
potential
creating
cartilage-mimicking
constructs,
thereby
opening
new
possibilities
repair.
In
this
review,
we
summarize
current
treatment
methods
their
limitations
addressing
articular
lesion,
various
strategies
features
tissue
engineering,
seed
cells
from
different
sources,
types
biomaterials.
We
also
explore
benefits,
challenges,
future
research
directions
within
field
engineering.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 28, 2025
Abstract
Inducibly
degradable
polymers
present
new
opportunities
to
integrate
tough
hydrogels
into
a
wide
range
of
biomaterials.
Rapid
and
inducible
degradation
enables
fast
transition
in
material
properties
without
sacrificing
integrity
prior
removal.
In
pursuit
bioorthogonal
chemical
modalities
that
will
enable
polymer
biologically
relevant
environments,
enamine
N
‐oxide
crosslinkers
are
developed
for
double
network
acrylamide‐based
polymer/alginate
hydrogels.
Bioorthogonal
dissociation
initiated
by
the
application
aqueous
diboron
solution
through
several
delivery
mechanisms
effectively
lead
degradation.
Their
B
2
(OH)
4
results
fracture
energy
half‐life
<10
min.
The
biocompatibility
reagent
is
assessed,
removability
strongly
adhered
on
mice
skin
evaluated.
Thermoresponsive
PNiPAAm/Alg
fabricated
as
chemically
intraoral
wound
dressing
demonstrated.
It
demonstrated
vivo
maximum
tolerated
dose
studies
administered
oral
gavage
well
tolerated.
Successful
integration
‐oxides
within
motifs
demonstrates
applicability
realm
chemistry
highlights
importance
induced
reactions
materials
science.
Materials Today Bio,
Год журнала:
2025,
Номер
31, С. 101641 - 101641
Опубликована: Март 8, 2025
In
recent
years,
hydrogel
microspheres
have
garnered
significant
attention
due
to
their
unique
structure
and
functionality,
demonstrating
substantial
potential
in
articular
cartilage
injury
repair.
This
paper
provides
a
comprehensive
overview
of
current
strategies
for
repair
summarizes
the
materials
preparation
methods
microspheres.
Furthermore,
it
highlights
multiple
roles
repair,
including
inflammation
control,
regulation
chondrocyte
metabolism,
drug
cell
delivery,
lubrication
improvement,
recruitment
endogenous
stem
cells.
Finally,
discusses
application
prospects
microspheres,
identifies
limitations
challenges,
offers
insights
guide
future
research
practical
applications
