Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 6, 2024
Abstract
Bone
marrow
stem
cells
(BMSCs)‐engineered
cartilage
(BEC)
shows
promise
for
clinically
repairing
defects.
However,
when
implanted
in
immunocompetent
large
animals,
BEC
becomes
susceptible
to
ossification
due
inflammatory
infiltration.
To
address
this,
a
nanofilm
isolation
approach
is
developed
enhance
BEC's
chondrogenic
stability.
Tacrolimus
(FK506),
known
its
immunosuppressive
effect,
integrated
into
adipic
dihydrazide
(ADH)‐modified
hyaluronic
acid
(HA),
creating
an
acid‐responsive
macromolecular
prodrug
called
FK506@HA‐ADH.
This
then
blended
with
poly(lactic‐co‐glycolic
acid)
(PLGA)
form
electrospun
FK506@HA/PLGA
nanofilm.
Goat‐derived
BMSCs
are
induced
vitro
BEC,
which
enclosed
within
the
and
subcutaneously
autologous
goats.
The
acted
as
physical
barrier,
preventing
immunocyte
Additionally,
response
acidic
environment
triggered
by
inflammation
gradual
degradation
of
PLGA,
FK506@HA‐ADH
cleaved,
releasing
FK506
needed.
released
effectively
countered
cytokines
promoted
cartilaginous
maturity.
These
combined
mechanisms
significantly
inhibited
hypertrophy
improved
stability
goat
model.
nanofilm‐based
strategy
established
niche,
successfully
endochondral
promoting
stable
formation
BEC.
advancements
crucial
translating
cell‐based
therapies
clinical
use
repair.
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 16, 2024
Abstract
Sports-related
injuries
often
cause
damage
to
the
articular
cartilage,
a
structure
characterized
by
absence
of
blood
vessels,
nerves,
and
lymphatics,
which
hinders
its
ability
heal.
Current
clinical
interventions
fall
short
in
reversing
cartilage
degeneration
or
regenerating
fibrocartilage,
leading
less
than
satisfactory
outcomes.
Furthermore,
use
hydrogel-based
stem
cell
therapies
has
been
hampered
poor
survival
transplanted
cells
inflamed
environment
injured
uncontrolled
differentiation
these
due
cytokines,
limiting
their
application.
In
our
study,
we
developed
novel
approach
using
biodegradable
peptide
hydrogel
that
incorporates
anti-inflammatory
exosomes
known
stimulate
growth
peptides
recruit
endogenous
site
injury.
This
system
sidesteps
need
for
exogenous
utilizing
body's
own
healing
mechanisms,
guided
specific
peptides.
Once
at
injury,
are
stimulated
differentiate
into
tissue
through
combination
TGF-β1
exosomes,
also
help
suppress
inflammation
promote
healing.
research
offers
promising
new
strategy
treating
sports-related
injuries,
presenting
more
effective
invasive
option
patients.
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 6, 2024
Abstract
Bone
marrow
stem
cells
(BMSCs)‐engineered
cartilage
(BEC)
shows
promise
for
clinically
repairing
defects.
However,
when
implanted
in
immunocompetent
large
animals,
BEC
becomes
susceptible
to
ossification
due
inflammatory
infiltration.
To
address
this,
a
nanofilm
isolation
approach
is
developed
enhance
BEC's
chondrogenic
stability.
Tacrolimus
(FK506),
known
its
immunosuppressive
effect,
integrated
into
adipic
dihydrazide
(ADH)‐modified
hyaluronic
acid
(HA),
creating
an
acid‐responsive
macromolecular
prodrug
called
FK506@HA‐ADH.
This
then
blended
with
poly(lactic‐co‐glycolic
acid)
(PLGA)
form
electrospun
FK506@HA/PLGA
nanofilm.
Goat‐derived
BMSCs
are
induced
vitro
BEC,
which
enclosed
within
the
and
subcutaneously
autologous
goats.
The
acted
as
physical
barrier,
preventing
immunocyte
Additionally,
response
acidic
environment
triggered
by
inflammation
gradual
degradation
of
PLGA,
FK506@HA‐ADH
cleaved,
releasing
FK506
needed.
released
effectively
countered
cytokines
promoted
cartilaginous
maturity.
These
combined
mechanisms
significantly
inhibited
hypertrophy
improved
stability
goat
model.
nanofilm‐based
strategy
established
niche,
successfully
endochondral
promoting
stable
formation
BEC.
advancements
crucial
translating
cell‐based
therapies
clinical
use
repair.
