Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 159081 - 159081
Опубликована: Дек. 1, 2024
Язык: Английский
Advanced Healthcare Materials, Год журнала: 2024, Номер 13(16)
Опубликована: Март 12, 2024
Abstract The management of diabetic wounds (DWs) continues to pose a significant challenge in the field medicine. DWs are primarily prevented from healing due damage macrophage efferocytosis and fibroblast dysfunction. Consequently, treatment strategy that involves both immunoregulation promotion extracellular matrix (ECM) formation holds promise for DWs. Nevertheless, existing methods necessitate complex interventions associated with increased costs, example, use cytokines cell therapy, which have limited effectiveness. In this study, new type ruthenium (IV) oxide nanoparticles (RNPs)‐laden hybrid hydrogel dressing double network Pluronic F127 F68 has been developed. Notably, demonstrates remarkable thermosensitivity, injectability, immunoregulatory characteristics, capability. RNPs effectively regulate fibroblasts macrophages cascade manner, stimulating differentiation while synergistically enhancing macrophage. character aids restoring intrinsic stability immune microenvironment wound facilitates essential remodeling ECM. This therefore offers novel approach treating through intercellular communication.
Язык: Английский
Процитировано
11
Advanced Healthcare Materials, Год журнала: 2025, Номер unknown
Опубликована: Фев. 11, 2025
Abstract The inability to heal on time is a key component of chronic wounds, which can result in economic, psychological, and physiological burdens. Hydrogels based amyloid imitate the extracellular matrix function as scaffolds for healing wounds. In this direction, wound dressing inspired by peptide‐based hydrogel fabricated here. results demonstrate that improves three essential components skin tissue regeneration: cell migration, proliferation, collagen remodeling, both vitro vivo. Furthermore, accelerates promotes closure within 9 15 d normal diabetic rats, respectively. Microscopic evaluation region demonstrates ultimate stages regeneration reformation toward compared untreated wound. Hematoxylin eosin‐stained hydrogel‐treated sites reveal faster dermal bridging, angiogenesis, epidermal repair acute conditions. creates an environment encourages growth fibroblasts release cytokines, decreasing inflammation with concomitant enhancement production at site injury. Thus, these findings suggest amyloid‐based be promising candidate application healing.
Язык: Английский
Процитировано
0
Biomedical Technology, Год журнала: 2025, Номер 9, С. 100071 - 100071
Опубликована: Фев. 21, 2025
Язык: Английский
Процитировано
0
Acta Biomaterialia, Год журнала: 2025, Номер unknown
Опубликована: Фев. 1, 2025
Delayed diabetic wound (DBW) healing is a severe complication of diabetes, characterized notably by peripheral sensory neuropathy. The underlying mechanism nerves and DBW remain unclear. Here, we demonstrate the role calcitonin gene-related peptide (CGRP) in regulating epithelialization angiogenesis DBW. Subsequently, design synthesis gelatin methacryloyl (GelMA-CGRP) hydrogel that slowly releases CGRP, evaluated its effect on promoting healing. results show CGRP abnormally downregulated DBW, ablation further delays This due to reduced M2 polarization decreased absence whereas local application GelMA-CGRP accelerates Mechanistic studies indicate promotes macrophage inhibiting p53 signaling pathway enhances endothelial cell function, thereby accelerating These findings suggest could provide novel therapeutic approach for treatment. STATEMENT OF SIGNIFICANCE: Current methods treating wounds have many limitations. Compared conventional dressings, hydrogels combined with drugs or biological factors promote become an important research direction recent years. study reveals key pathogenesis wounds. found pathway, We utilized carrier properties GelMA develop material delivers effectively treats demonstrates strong biocompatibility antimicrobial properties, offering treatment
Язык: Английский
Процитировано
0
ACS Biomaterials Science & Engineering, Год журнала: 2025, Номер unknown
Опубликована: Март 20, 2025
Excessive oxidative stress, chronic inflammation, and impaired vascularization are the main barriers to diabetic wound repair. A decellularized extracellular matrix (dECM) with a native ECM structure is promising biomaterial candidate for healing. However, traditional decellularization process (reagents) can diminish structural stability, mechanical properties, bioactive components of dECM. To address these issues, we developed an intrinsically kelp scaffold (Im-Gly2) using natural gentle deep eutectic solvents (DES) accelerating Im-Gly2 had stable porous 3D (80.7 μm) suitable which could support cell growth, proliferation, migration. Due retention fucoidan, polyphenols (735.3 μg/g), flavonoids, demonstrated intrinsic antioxidant immunomodulatory effects. It effectively reduced reactive oxygen species (ROS) production in RAW264.7 macrophages promoted their differentiation into M2 phenotype. Notably, tube formation through paracrine mechanisms by inducing expression transforming proliferative cytokines from macrophage. In vivo, accelerated healing wounds alleviating angiogenesis, granulation tissue formation, collagen deposition, re-epithelialization. Taken together, our study provides novel strategy fabricating dECM without cross-linking exogenous substances
Язык: Английский
Процитировано
0
Journal of Advanced Research, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
ACS Biomaterials Science & Engineering, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Hydrogel wound dressings have emerged as a promising solution for healing due to their excellent mechanical and biochemical properties. Over recent years, there has been significant progress in expanding the variety of raw materials used hydrogel formulation along with development advanced modification techniques design approaches that enhance performance. However, comprehensive review encompassing diverse polymer strategies innovations is still lacking literature. This summarizes use natural polymers (e.g., chitosan, gelatin, sodium alginate, hyaluronic acid, dextran) synthetic poly(vinyl alcohol), polyethylene glycol, Pluronic F-127, poly(N-isopropylacrylamide), polyacrylamide, polypeptides) dressings. We further explore advantages limitations these discuss various strategies, including cationic modification, oxidative double-bond catechol etc. The also addresses principles synthesis methods, aligning modifications specific requirements healing. Finally, we future challenges opportunities hydrogel-based
Язык: Английский
Процитировано
0
Bioactive Materials, Год журнала: 2025, Номер 50, С. 30 - 46
Опубликована: Апрель 5, 2025
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 136699 - 136699
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Авг. 13, 2024
Abstract Defects in apoptotic cell clearance, or efferocytosis, can cause inflammatory diseases and prevent tissue repair due part to inducing a pro-repair transcriptional program phagocytic cells like macrophages. While the cellular machinery metabolic pathways involved efferocytosis have been characterized, precise efferocytic response of macrophages is dependent on identity macromolecular cues cells, complex microenvironment which occurs. Here, we find that undergoing active mid-stage mouse skin wounds vivo display gene program, while fibroblasts vitro also induces an transcription response. These data provide resource for understanding how wound environment influences macrophage will be useful future investigations define role during repair.
Язык: Английский
Процитировано
0