UiO-66 MOF Nanozymes as Affinity Columns for Proteomics Applications

ACS Applied Nano Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

Язык: Английский

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In‐Tip Nanoreactors for Simultaneous Proteolysis and Enrichment of Phosphorylated Peptides

Analytical Science Advances, Год журнала: 2025, Номер 6(1)

Опубликована: Март 12, 2025

ABSTRACT Protein phosphorylation introduces negative charges on the hydroxyl groups of serine, threonine, and tyrosine residues, reducing ionization efficiency phosphorylated peptides. The low abundance peptides often diminishes their detection using mass spectrometry. To enhance identification low‐abundance peptides, an enrichment step was used, which complicated high‐throughput analysis proteomes. In this study, we developed a titanium dioxide surface‐modified macroporous silicon encapsulated micropipette tips, loaded with trypsin, to integrate rapid enzymatic protein hydrolysis selective extraction within microfluidic enzyme reactor. This streamlined approach simplified sample preparation process, combining hydrolysis, separation while maintaining high efficiency. method enabled comprehensive complex cancer cell line samples in 1–2 h. Successful from mixtures achieved matrix‐assisted laser desorption/ionization‐time‐of‐flight‐mass application may provide potential for phosphoproteomics advance study modifications.

Язык: Английский

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In Situ Metal–Organic Framework Growth in Serum Encapsulates and Depletes Abundant Proteins for Integrated Plasma Proteomics

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Protein biomarkers in human serum provide critical insights into various physiological conditions and diseases, enabling early diagnosis, prognosis, personalized treatment. However, detecting low-abundance protein is challenging due to the presence of highly abundant proteins that make up ∼99% plasma proteome. Here, we report use situ metal–organic framework (MOF) growth effectively deplete for integrated proteomic analysis. Through biomolecule-mediated nucleation a zeolitic imidazolate (ZIF-8), are selectively encapsulated within ZIF-8 removed from via centrifugation, leaving depleted fraction supernatant. Bottom-up proteomics analysis confirmed significant depletion topmost proteins, many at levels exceeding 95%. Such enabled identification 277 total supernatant (uncaptured) single-shot analysis, including 54 were only identified after depletion, 12 drug targets, potential disease biomarkers. Top-down characterization captured uncaptured fractions proteoform-level this method not biased toward any specific proteoform individual proteins. These results demonstrate MOF can high-abundance simple, low cost, one-pot synthesis enable top-down bottom-up

Язык: Английский

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Cluster Nuclearity Determines Substrate Adsorption/Desorption Dynamics and Peptidase Activity of UiO-66 Nanozymes

Chemistry of Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Язык: Английский

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