International Immunopharmacology, Год журнала: 2024, Номер 146, С. 113864 - 113864
Опубликована: Дек. 19, 2024
Язык: Английский
International Journal of Nanomedicine, Год журнала: 2025, Номер Volume 20, С. 1401 - 1423
Опубликована: Фев. 1, 2025
Abstract: Kidney stones constitute a common condition impacting the urinary system. In clinical diagnosis and management, traditional surgical interventions pharmacological treatments are primarily utilized; however, these methods possess inherent limitations. Presently, field of nanomedicine is undergoing significant advancements. The application nanomaterials in biosensors enables accurate assessment ion composition. Furthermore, contrast agents developed from materials can improve signal-to-noise ratio enhance image clarity. By mitigating oxidative stress-induced cellular damage, inhibit formation kidney efficacy drug delivery as effective carriers. Additionally, by modifying physical chemical properties bacteria, effectively eliminate bacterial presence, thereby preventing severe complications. This review explores advancements technology related to early detection risk factors, diagnosis, treatment their associated Keywords: nanomaterials, stones, stress, biomaterial
Язык: Английский
Процитировано
1
Advanced Healthcare Materials, Год журнала: 2025, Номер unknown
Опубликована: Янв. 19, 2025
Abstract Liver ischemia and reperfusion (I/R) injury is a reactive oxygen species (ROS)‐related disease that occurs during liver transplantation resection hinders postoperative function recovery. Current approaches to alleviate I/R have limited effectiveness due the short circulation time, poor solubility, severe side effects of conventional antioxidants anti‐inflammatory drugs. Herein, universal strategy proposed fabricate Trojan horse‐like biohybrid nanozyme (THBN) with hepatic‐targeting capabilities. Tannic acid (TA) mediates adeno‐associated virus (AAV8) decoration onto 2D Ti 3 C 2 nanosheets, resulting in THBN size 116.2 ± 9.5 nm. Remarkably, exhibits catalase (CAT)‐like activity, broad‐spectrum ROS scavenging activity targeted delivery tissue owing presence AAV8. Both vivo vitro experiments confirmed efficacy attenuating by mitigating inflammation oxidative stress inhibiting hepatocellular apoptosis. RNA‐seq analysis suggests may activating PKC pathway. The effective targeting therapeutic capabilities represent an advancement nanotherapeutics for hepatic ischemia‒reperfusion injury, shedding light on promising potential this next‐generation nanotherapeutic approach.
Язык: Английский
Процитировано
0
Biochemical Genetics, Год журнала: 2025, Номер unknown
Опубликована: Фев. 1, 2025
The predominant component of kidney stone is calcium oxalate monohydrate (COM), a fact widely acknowledged. Although rodent models are frequently used to induce (CaOx) crystallization, further exploration Randall's plaques (RPs) in these still needed. We first selected the GSE89028 and GSE75542 datasets from Gene Expression Omnibus (GEO) database identify commonly differentially expressed genes (co-DEGs). Based on co-DEGs, we conducted Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analyses significantly enriched pathways. Additionally, performed Set Enrichment Analysis (GSEA) validate In order hub genes, established network protein–protein interactions (PPI). Finally, real-time PCR Western blot findings bioinformatics analysis. 28 co-DEGs two datasets. enrichment analysis using GO, KEGG, GSEA revealed significant chemokine-related signaling histogram showed that three chemokine factor-related were involved multiple Cytohubba confirm presence genes. Subsequently, external quantitative polymerase chain reaction (qRT-PCR) demonstrated upregulation CCL2, CXCL1, CXCL2 HK-2 cells following CaOx treatment compared control group (p < 0.05). Our study upon stimulation by CaOx, renal tubular epithelial release chemokines, including CXCL2. This chemokines accompanied activation pathways such as TNF IL-17. These may provide new directions for future research Kidney Stone Disease.
Язык: Английский
Процитировано
0
Biomaterials Science, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Background: Calcium oxalate (CaOx) crystal deposition and its resultant cellular oxidative damage inflammation are important causes of renal stone formation. It is clinically to conduct research on multifunctional anti-stone drugs targeting these predisposing factors. Methods: We modified natural Undaria pinnatifida polysaccharide (UPP0) by sulfation via the sulfur trioxide-pyridine method, resulting in four sulfated polysaccharides with varying sulfate group (-OSO3-) contents: 1.59% (UPP0), 6.03% (UPP1), 20.83% (UPP2), 36.39% (UPP3), compared their differences inhibition crystalline formation, injury, process formation at chemical levels. Results: The UPPS were able inhibit nucleation, growth aggregation CaOx crystals vitro. Among them, UPP3 maximum content showed greatest crystallization ability. nucleation a concentration 0.5 mg mL-1 as high 80.21% 72.34%, respectively. size regulated was significantly reduced from 25.9 ± 2.8 μm 5.9 1.2 μm. Furthermore, observed up-regulate expression antioxidant enzyme superoxide dismutase (SOD) cells, reduce levels ROS malonaldehyde (MDA), enhance lysosomal integrity, decrease intracellular Ca2+ levels, decline mitochondrial membrane potential, production inflammatory factors (TNF-α, MCP-1, IL-18, IL-1β), ultimately cell apoptosis. Conclusion: combine multiple biological functions regulation, anti-inflammatory, have potential prevention kidney stones. Sulfation modification can improve activity UPP0 provide reference for screening optimization methods drugs.
Язык: Английский
Процитировано
0
International Immunopharmacology, Год журнала: 2025, Номер 150, С. 114302 - 114302
Опубликована: Фев. 18, 2025
Язык: Английский
Процитировано
0
International Immunopharmacology, Год журнала: 2024, Номер 146, С. 113864 - 113864
Опубликована: Дек. 19, 2024
Язык: Английский
Процитировано
1