ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 25, 2024
Uniform,
mesoporous
copper(II)
oxide
nanospindles
(CuO
NSs)
were
synthesized
via
a
method
based
on
templated
hydrothermal
oxidation
of
copper
in
the
presence
monodisperse
poly(glycerol
dimethacrylate-co-methacrylic
acid)
nanoparticles
(poly(GDMA-co-MAA)
NPs).
Subsequent
decoration
CuO
NSs
with
CaO2
nanoshell
(CuO@CaO2
yielded
nanozyme
capable
Cu(I)/Cu(II)
redox
cycling.
Activation
cycle
by
exogenously
generated
H2O2
from
significantly
enhanced
glutathione
(GSH)
depletion.
CuO@CaO2
exhibited
2-fold
higher
GSH
depletion
rate
compared
to
pristine
NSs.
The
generation
oxygen
due
catalase
(CAT)-like
decomposition
resulted
self-propelled
diffusion
behavior,
characteristic
fueled
nanomotor.
These
nanostructures
both
peroxidase
(POD)-like
and
CAT-like
activities
self-production
aqueous
media
chemical
reaction
between
water.
Usage
self-supplied
POD-like
activity
amplified
toxic
hydroxyl
(•OH)
radicals,
enhancing
chemodynamic
effect
within
tumor
microenvironment
(TME).
provided
source
O2
alleviate
hypoxic
conditions
TME.
Under
near-infrared
laser
irradiation,
photothermal
conversion
properties,
temperature
elevation
25
°C.
combined
led
more
effective
production
•OH
radicals
cell
culture
medium.
function
was
further
an
elevated
temperature.
To
assess
therapeutic
potential,
loaded
photosensitizer,
chlorine
e6
(Ce6),
evaluated
against
T98G
glioblastoma
cells.
synergistic
combination
photodynamic,
photohermal,
modalities
using
CuO@CaO2@Ce6
death
than
90%
under
vitro
conditions.
Sensors,
Год журнала:
2024,
Номер
24(20), С. 6701 - 6701
Опубликована: Окт. 18, 2024
This
work
reported
gold
nanoparticles
(AuNPs)-based
colorimetric
immunoassay
with
the
Cu-based
metal–organic
framework
(MOF)
to
load
pyrroloquinoline
quinone
(PQQ)
for
catalytic
oxidation
of
cysteine.
In
this
method,
both
Cu2+
and
PQQ
in
MOF
could
promote
inducer
cysteine
by
redox
cycling,
thus
limiting
cysteine-induced
aggregation
AuNPs
achieving
dual
signal
amplification.
Specifically,
recombinant
carcinoembryonic
antigen
(CEA)
targets
were
anchored
on
through
metal
coordination
interactions
between
hexahistidine
(His6)
tag
CEA
unsaturated
sites
MOF.
The
CEA/PQQ-loaded
be
captured
antibody-coated
ELISA
plate
catalyze
However,
once
target
samples
bound
antibody
immobilized
surface,
attachment
would
limited.
Cysteine
remaining
solution
trigger
cause
a
color
change
from
red
blue.
concentration
was
positively
related
AuNPs.
signal-on
competitive
plasmonic
exhibited
low
detection
limit
linear
range
0.01–1
ng/mL.
Note
that
most
proteins
commercial
kits
are
His6
N-
or
C-terminal,
so
provide
sensitive
platform
biomarkers.
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 3, 2025
The
characteristics
of
the
tumor
microenvironment
(TME)
have
a
close
and
internal
correlation
with
effect
cancer
immunotherapy,
significantly
affecting
progression
metastasis
cancer.
rational
design
nanoenzymes
that
possess
ability
to
respond
regulate
TME
is
driving
new
direction
in
catalytic
immunotherapy.
In
this
study,
we
designed
multifunctional
manganese
(Mn)-based
nanoenzyme
responsive
acidic
pH
overxpressed
H2O2
at
site
holds
capability
modulating
hypoxic
immunosuppressive
for
synergistic
anti-tumor
photothermal/photodynamic/immunotherapy.
We
found
artificial
promoted
peroxidase-like
catalase-like
activities
catalyzed
in-situ
decomposition
H2O2,
metabolic
waste
product
TME,
into
∙OH
O2,
resulting
ROS
burst
killing
tumors
relieving
enhance
therapy.
Besides
photothermal
enhancement
burst-induced
immunogenic
cell
death,
combination
Mn2+
released
from
Mn-based
programmed
death-ligand
1
blockade
triggered
significant
immune
response.
A
remarkable
vivo
therapeutic
was
achieved
effective
inhibition
primary
growth
lung
metastasis.
Therefore,
TME-responsive
offers
safe
efficient
platform
reversing
achieving
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 21, 2025
Organophosphorus
pesticides
(OPs)
are
widely
used
in
agricultural
production,
posing
a
great
threat
to
human
health
and
the
environment.
Given
that
different
OPs
present
toxicology
toxicities,
identifying
individual
pesticide
residues
becomes
important
for
assessing
food
safety
environmental
implications.
In
this
work,
kinetics
difference-driven
analyte
hydrolysis
strategy
is
proposed
first
time
validated
identify
p-nitrophenyl
by
developing
an
organophosphorus
hydrolase-like
nanozyme-coded
sensor
array.
Ultrasmall
bare
CeO2
nanoparticles
were
synthesized
employed
as
only
sensing
unit
catalyze
of
multiple
analytes.
With
catalytic
preferences
differences
under
identical
reaction
conditions,
five
common
analogues
(methyl-paraoxon,
paraoxon,
methyl-parathion,
parathion,
fenitrothion)
offered
discriminable
colors.
By
coupling
color
fingerprints
with
pattern
recognition,
accurate
identification
their
mixtures
at
variety
concentrations
ratios
was
verified
laboratory
practical
scenarios.
Attractively,
apart
from
excellent
performance
convenient
operation,
hydrolytic
presents
strong
resistance
against
redox
substances
often
cause
interference
previous
oxidoreductase-based
arrays.
Our
study
provides
new
paradigm
discriminating
specific
precisely,
showing
promising
applications
multitarget
analysis
complex
matrices.
Abstract
Mimicking
the
structure
of
natural
enzyme
active
sites
offers
a
promising
strategy
for
rational
design
nanozymes.
However,
this
biomimetic
approach
predominantly
focuses
on
replicating
configuration
metal
center
in
enzymes,
often
overlooking
critical
influence
catalytic
site's
microenvironment.
Here,
inspired
by
and
coordination
microenvironment
organophosphorus
hydrolase
(OPH),
Ce
2
O
CN
/NC,
novel
cerium‐based
nanozyme
is
first
reported
to
mimic
OPH.
In
species
serve
as
sites,
while
adjacent
N
site
([N═C═N]
2−
)
functions
general
base,
mimicking
histidine
enzymes
facilitate
hydrolysis
process.
Using
paraoxon
model
target,
/NC
demonstrates
rapid
dephosphorylation
phosphotriester
across
wide
range
temperatures
pH
values,
significantly
outperforming
OPH
CeO
nanoparticles.
The
systematic
experiments
theoretical
calculations
reveal
underlying
mechanisms
responsible
enhanced
OPH‐mimicking
performance.
Capitalizing
its
phosphatase‐like
activity,
successfully
employed
develop
colorimetric
biosensor
selective
detection
pesticides.
This
study
holds
great
promise
developing
efficient
nanozymes
broadens
Ce‐based
Traditional
biological
detection
methods
rely
on
signal
amplification
strategies
such
as
enzymatic
catalysis
or
nucleic
acid
amplification.
However,
their
efficiency
decreases
in
low-temperature
environments,
compromising
sensitivity.
To
break
the
loss
of
enzyme
catalytic
activity
at
low
temperatures,
research
cold-adaptive
nanozymes
has
attracted
much
attention.
Till
now,
only
a
few
have
been
reported
to
cold-resistant
properties.
Here,
new
type
cold-adapted
nanozyme
was
constructed
by
engineering
perovskite
hydroxide.
The
not
boosted
oxidase-like
2
orders
magnitude
but
also
retained
excellent
performance
0
°C.
This
enhanced
may
be
attributed
increase
manganese
content,
vacancy
oxygen,
and
tetravalent
manganese.
Then,
robust
adaptive
biosensor
established
with
cold
nanozyme.
Notably,
sulfide
ion,
ascorbic
acid,
alkaline
phosphatase,
cellular
glutathione
probe
less
affected
temperature
reduction,
sensitivity
for
ALP
°C
is
better
than
that
commercial
kit.
Finally,
further
used
construct
paper-based
H2S
gas
colorimetric
probe.
study
develops
broadens
application
scenarios
nanozymes.
