The applying of immuno-RCA for the high-sensitivity detection of the ABO blood group antibodies on the printed glycoarray DOI Creative Commons

Ekaterina E. Kornilova,

Ruslan R. Kutukov,

Polykova M. Svetlana

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 13, 2024

Detecting small amounts of analytes, especially antibodies, presents a significant challenge in high-throughput methods. Fortunately, nucleic acid amplification techniques provide promising solution. We have successfully developed and tested an innovative technology for detecting anti-glycan utilizing printed glycan array combined with rolling circle reaction based on the ABO blood group antibody model. This breakthrough has dramatically enhanced sensitivity our immunoassay, improving it by over order magnitude allowing us to detect concentrations as low 1 ng/ml. advancement opens new approaches research clinical applications, making previously undetectable analytes accessible study.

Язык: Английский

The applying of immuno-RCA for the high-sensitivity detection of the ABO blood group antibodies on the printed glycoarray DOI Creative Commons

Ekaterina E. Kornilova,

Ruslan R. Kutukov,

Polykova M. Svetlana

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 13, 2024

Detecting small amounts of analytes, especially antibodies, presents a significant challenge in high-throughput methods. Fortunately, nucleic acid amplification techniques provide promising solution. We have successfully developed and tested an innovative technology for detecting anti-glycan utilizing printed glycan array combined with rolling circle reaction based on the ABO blood group antibody model. This breakthrough has dramatically enhanced sensitivity our immunoassay, improving it by over order magnitude allowing us to detect concentrations as low 1 ng/ml. advancement opens new approaches research clinical applications, making previously undetectable analytes accessible study.

Язык: Английский

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