Fibrosis Drug Efficacy Assessment Based on Microfluidic Mechanical Property Evaluation of Spheroid Models DOI Open Access

B.H. Kim,

Jeong Yeon Kim, Hye Won Kim

и другие.

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 16, 2025

Abstract Fibrotic diseases, such as pulmonary fibrosis, pose significant challenges in both research and treatment. To address the limitations of existing systems, a novel collision‐based spheroid mechanical property assessment system is developed. The utilizes inertial fluid dynamics to induce controlled collisions through uniformly sized spheroids, allowing strain be measured via high‐speed cameras. In this study, first validated using HEK293T spheroids optimize flow velocity, followed by an analysis deformability differences between two cell types related fibrosis (Calu‐1 MRC‐5). A co‐culture model comprising lung cells, endothelial fibroblast different rations further developed, depending on composition observed. Finally, are treated with TGF‐β1(Transforming Growth Factor‐β1), factor known activate cells excessive extra cellular matrix (ECM) accumulation, Nintedanib, anti‐fibrotic drug, assess changes properties. These results effectively reflect properties driven cell‐cell cell‐ECM interactions highlight correlation mechanics progression fibrotic disease. This not only contributes deeper understanding but also serves powerful platform for accelerating development therapies.

Язык: Английский

Fibrosis Drug Efficacy Assessment Based on Microfluidic Mechanical Property Evaluation of Spheroid Models DOI Open Access

B.H. Kim,

Jeong Yeon Kim, Hye Won Kim

и другие.

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 16, 2025

Abstract Fibrotic diseases, such as pulmonary fibrosis, pose significant challenges in both research and treatment. To address the limitations of existing systems, a novel collision‐based spheroid mechanical property assessment system is developed. The utilizes inertial fluid dynamics to induce controlled collisions through uniformly sized spheroids, allowing strain be measured via high‐speed cameras. In this study, first validated using HEK293T spheroids optimize flow velocity, followed by an analysis deformability differences between two cell types related fibrosis (Calu‐1 MRC‐5). A co‐culture model comprising lung cells, endothelial fibroblast different rations further developed, depending on composition observed. Finally, are treated with TGF‐β1(Transforming Growth Factor‐β1), factor known activate cells excessive extra cellular matrix (ECM) accumulation, Nintedanib, anti‐fibrotic drug, assess changes properties. These results effectively reflect properties driven cell‐cell cell‐ECM interactions highlight correlation mechanics progression fibrotic disease. This not only contributes deeper understanding but also serves powerful platform for accelerating development therapies.

Язык: Английский

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