Advanced Healthcare Materials, Год журнала: 2024, Номер 14(4)

Опубликована: Ноя. 15, 2024

Understanding the myofibroblast microenvironment is critical to developing therapies for fibrotic diseases. Here development of a novel human tendon-on-a-chip (hToC) reported model this crosstalk in peritendinous adhesions, which currently lacks biological therapies. The hToC facilitates cellular and paracrine interactions between vascular component, contains endothelial cells monocytes, tissue hydrogel component that houses tendon macrophages. It found replicates some aspects vivo inflammatory phenotypes preclinical clinical samples, including activated mTOR signaling, inflammation, contraction induced by activation, cytokines secretion, transendothelial migration monocytes hydrogel. Transcriptional analysis demonstrates significant overlap enriched pathways with tenolysis activation signaling. Rapamycin suppresses inflammation phenotype hToC, provides proof-of-concept its utility as an vitro tool investigating multicellular fibrosis testing therapeutics mitigate it.

Язык: Английский