Multiplex Chroma Response Wearable Hydrogel Patch: Visual Monitoring of Urea in Body Fluids for Health Prognosis

Analytical Chemistry, Год журнала: 2023, Номер 95(7), С. 3587 - 3595

Опубликована: Фев. 8, 2023

Visual wearable devices can rapid intuitively monitor biomarkers in body fluids to indicate the human health status and provide valuable reference for further medical diagnosis. However, unavoidable interference factors such as skin color, natural light, background luminescence interfere with visualization accuracy of flexible devices, limiting their practical sensing application. Here, we designed a patch via an embedded upconversion optical probe 3D porous polyacrylamide hydrogel, exhibiting multiplex chroma response urea based on inner filter effect, which overcomes susceptibility external conditions due its near-infrared excited improves resolution visual sensing. Furthermore, highly compatible portable platform combined smartphone was achieve situ quantitative analysis urea. The limit detection values hydrogel sensor are low 1.4 30 μM respectively, practicality different scenarios. provides convenient accurate strategy has potential be developed into point-of-care device disease early warning clinical

Язык: Английский

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Recent Advances in Graphene Field‐Effect Transistor Toward Biological Detection

Advanced Functional Materials, Год журнала: 2024, Номер 34(44)

Опубликована: Май 22, 2024

Abstract Recently, field‐effect transistors (FETs) have emerged as a novel type of multiparameter, high‐performance, highly integrated platform for biochemical detection, leveraging their classical three‐terminal structure, working principles, and fabrication methods. Notably, graphene materials, known exceptional electrical optical properties well biocompatibility, serve fundamental component these devices, further enhancing advantages in biological detection. This review places special emphasis on recent advancements transistor (GFET)‐based biosensors focuses four main areas: i) the basic concepts FETs specific GFETs; ii) various state‐of‐the‐art approaches to enhance performance GFET‐based terms operating principles “3S”—stability, sensitivity, specificity; iii) multiplexed detection strategies biosensors; iv) current challenges future perspectives field biosensors. It is hoped that this article can profoundly elucidate development GFET inspire broader audience.

Язык: Английский

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Using a crumpled graphene field-effect transistor for ultrasensitive SARS-CoV-2 detection via its papain-like protease

Chemical Engineering Journal, Год журнала: 2025, Номер 505, С. 159386 - 159386

Опубликована: Янв. 8, 2025

Язык: Английский

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Catapult-like DNA actuator for highly stable and ultrasensitive detection of urinary miRNA

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 159806 - 159806

Опубликована: Янв. 1, 2025

Язык: Английский

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Advances in lithographic techniques for precision nanostructure fabrication in biomedical applications

Discover Nano, Год журнала: 2023, Номер 18(1)

Опубликована: Дек. 11, 2023

Abstract Nano-fabrication techniques have demonstrated their vital importance in technological innovation. However, low-throughput, high-cost and intrinsic resolution limits pose significant restrictions, it is, therefore, paramount to continue improving existing methods as well developing new overcome these challenges. This is particularly applicable within the area of biomedical research, which focuses on sensing, increasingly at point-of-care, a way improve patient outcomes. Within this context, review latest advances main emerging patterning including two-photon, stereo, electrohydrodynamic, near-field electrospinning-assisted, magneto, magnetorheological drawing, nanoimprint, capillary force, nanosphere, edge, nano transfer printing block copolymer lithographic technologies for micro- nanofabrication. Emerging enabling structural chemical fabrication are categorised along with prospective physical techniques. Established briefly outlined novel compared these, summarising specific advantages shortfalls alongside current lateral amenability mass production, evaluated terms process scalability cost. Particular attention drawn potential breakthrough application areas, predominantly studies, laying platform tangible paths towards adoption alternative or combination established techniques, depends needs end-user including, instance, tolerance inherent limits, fidelity reproducibility.

Язык: Английский

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CRISPR‐Mediated Profiling of Viral RNA at Single‐Nucleotide Resolution

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(30)

Опубликована: Май 23, 2023

Mass pathogen screening is critical to preventing the outbreaks and spread of infectious diseases. The large-scale epidemic COVID-19 rapid mutation severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus have put forward new requirements for detection identification techniques. Here, we report a CRISPR-based Amplification-free Viral RNA Electrical Detection platform (CAVRED) SARS-CoV-2 variants. A series CRISPR assays were designed amplify CRISPR-Cas system's ability discriminate between mutant wild genomes with single-nucleotide difference. identified viral information was converted into readable electrical signals through field-effect transistor biosensors achievement highly sensitive single-base mutations. CAVRED can detect genome as low 1 cp μL-1 within 20 mins without amplification, this value comparable limit real-time quantitative polymerase chain reaction. Based on excellent ability, an 8-in-1 array constructed realized 40 simulated throat swab samples variants 95.0 % accuracy. advantages accuracy, sensitivity, fast speed promise its application in screening.

Язык: Английский

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Rapid Identification and Monitoring of Multiple Bacterial Infections Using Printed Nanoarrays

Advanced Materials, Год журнала: 2023, Номер 35(12)

Опубликована: Янв. 10, 2023

Fast and accurate detection of microbial cells in clinical samples is highly valuable but remains a challenge. Here, simple, culture-free diagnostic system developed for direct pathogenic bacteria water, urine, serum using an optical colorimetric biosensor. It consists printed nanoarrays chemically conjugated with specific antibodies that exhibits distinct color changes after capturing target pathogens. By utilizing the internal capillarity inside evaporating droplet, preconcentration achieved within few minutes to enable rapid identification more efficient bacterial More importantly, scattering signals are significantly amplified by due strong near-field localization, which supports visualizable analysis growth, reproduction, cell activity at single-cell level. Finally, addition high selectivity, this nanoarray-based biosensor also capable quantification continuous monitoring load on food over broad linear range, limit 10 CFU mL-1 . This work provides accessible user-friendly tool point-of-care testing pathogens many environmental applications, possibly enables breakthrough early prevention treatment.

Язык: Английский

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Interface‐Engineered Field‐Effect Transistor Electronic Devices for Biosensing

Advanced Materials, Год журнала: 2023, Номер unknown

Опубликована: Дек. 4, 2023

Promising advances in molecular medicine have promoted the urgent requirement for reliable and sensitive diagnostic tools. Electronic biosensing devices based on field-effect transistors (FETs) exhibit a wide range of benefits, including rapid label-free detection, high sensitivity, easy operation, capability integration, possessing significant potential application disease screening health monitoring. In this perspective, tremendous efforts achievements development high-performance FET biosensors past decade are summarized, with emphasis interface engineering FET-based electrical platforms biomolecule identification. First, an overview strategies modulation recognition element design is discussed detail. For further step, applications vitro detection real-time monitoring biological systems comprehensively reviewed. Finally, key opportunities challenges electronic discussed. It anticipated that comprehensive understanding will inspire additional techniques developing highly sensitive, specific, stable as well emerging designs next-generation electronics.

Язык: Английский

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Carbon-Based Fluorescent Nano-Biosensors for the Detection of Cell-Free Circulating MicroRNAs

Biosensors, Год журнала: 2023, Номер 13(2), С. 226 - 226

Опубликована: Фев. 4, 2023

Currently, non-communicable diseases (NCDs) have emerged as potential risks for humans due to adopting a sedentary lifestyle and inaccurate diagnoses. The early detection of NCDs using point-of-care technologies significantly decreases the burden will be poised transform clinical intervention healthcare provision. An imbalance in levels circulating cell-free microRNAs (ccf-miRNA) has manifested NCDs, which are passively released into bloodstream or actively produced from cells, improving efficacy disease screening providing enormous sensing potential. effective ccf-miRNA continues significant technical challenge, even though sophisticated equipment is needed analyze readouts expression patterns. Nanomaterials come light solution they provide advantages over other widely used diagnostic techniques measure miRNAs. Particularly, CNDs-based fluorescence nano-biosensors great interest. Owing excellent characteristics CNDs, developing such sensors ccf-microRNAs been much more accessible. Here, we critically examined recent advancements fluorescence-based CNDs biosensors, including tools manufacturing these biosensors. Green synthesis methods scaling up high-quality, fluorescent natural source discussed. various surface modifications that help attach biomolecules utilizing covalent conjugation multiple applications, self-assembly, sensing, imaging, analyzed. current review particular interest researchers interested materials chemistry, nanomedicine, related fields, focus on ccf-miRNAs applications medical field.

Язык: Английский

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Flexible Point-of-Care Electrodes for Ultrasensitive Detection of Bladder Tumor-Relevant miRNA in Urine

Analytical Chemistry, Год журнала: 2023, Номер 95(3), С. 1847 - 1855

Опубликована: Янв. 6, 2023

Portable point-of-care testing (POCT) is currently drawing enormous attention owing to its great potential for disease diagnosis and personal health management. Electrochemical biosensors, with the intrinsic advantages of cost-effectiveness, fast response, ease miniaturization, integration, are considered as one most promising candidates POCT application. However, clinical application electrochemical biosensors-based hindered by decreased detection sensitivity due low abundance disease-relevant biomolecules in extremely complex biological samples. Herein, we construct a flexible biosensor based on single-stranded DNA functionalized single-walled carbon nanotubes (ssDNA-SWNTs) high stability miRNA-21 human urine achieve bladder cancer (BCa) classification. The ssDNA-SWNT electrodes 2D interconnected network structure exhibit electrical conductivity, thus enabling ultrasensitive limit 3.0 fM. Additionally, flexibility endows biosensors capability even under large bending deformations. In cohort 40 BCa patients at stages I–III 44 negative control samples, constructed could detect 92.5% sensitivity, an 88.6% specificity, classify overall accuracy 81.0%. also be utilized treatment efficiency assessment recurrence monitoring. Owing their excellent stability, designed this work propose strategy realize samples personalized healthcare.

Язык: Английский

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