Abstract
Chemical
communication
via
neurotransmitters
is
central
to
brain
functions.
Nevertheless,
in
vivo
real‐time
monitoring
of
released
the
brain,
especially
electrochemically
inactive
molecules,
remains
a
great
challenge.
In
this
work,
novel
needle
field‐effect
transistor
(FET)
microsensor
based
on
an
acupuncture
proposed,
which
demonstrated
be
capable
dopamine
molecules
as
well
neuropeptide
Y
vivo.
The
FET
microstructure
fabricated
by
successively
wrapping
insulating
layer
and
gold
top
needle,
where
Au
served
source
drain,
respectively.
After
assembling
reduced
graphene
oxide
(RGO)
between
drain
electrodes,
specific
aptamer
immobilized
RGO,
making
needle‐FET
biosensor
highly
selective
sensitive
monitor
from
rat
even
Parkinson's
diseases
model.
Furthermore,
applied
detect
variety
targets
including
hormones,
proteins,
nucleic
acid.
By
constructing
sensing
interface
implanting
sensor
rat's
for
detection,
work
provides
new
sight
domain
further
expands
species
detection.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(7), С. 3587 - 3595
Опубликована: Фев. 8, 2023
Visual
wearable
devices
can
rapid
intuitively
monitor
biomarkers
in
body
fluids
to
indicate
the
human
health
status
and
provide
valuable
reference
for
further
medical
diagnosis.
However,
unavoidable
interference
factors
such
as
skin
color,
natural
light,
background
luminescence
interfere
with
visualization
accuracy
of
flexible
devices,
limiting
their
practical
sensing
application.
Here,
we
designed
a
patch
via
an
embedded
upconversion
optical
probe
3D
porous
polyacrylamide
hydrogel,
exhibiting
multiplex
chroma
response
urea
based
on
inner
filter
effect,
which
overcomes
susceptibility
external
conditions
due
its
near-infrared
excited
improves
resolution
visual
sensing.
Furthermore,
highly
compatible
portable
platform
combined
smartphone
was
achieve
situ
quantitative
analysis
urea.
The
limit
detection
values
hydrogel
sensor
are
low
1.4
30
μM
respectively,
practicality
different
scenarios.
provides
convenient
accurate
strategy
has
potential
be
developed
into
point-of-care
device
disease
early
warning
clinical
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(44)
Опубликована: Май 22, 2024
Abstract
Recently,
field‐effect
transistors
(FETs)
have
emerged
as
a
novel
type
of
multiparameter,
high‐performance,
highly
integrated
platform
for
biochemical
detection,
leveraging
their
classical
three‐terminal
structure,
working
principles,
and
fabrication
methods.
Notably,
graphene
materials,
known
exceptional
electrical
optical
properties
well
biocompatibility,
serve
fundamental
component
these
devices,
further
enhancing
advantages
in
biological
detection.
This
review
places
special
emphasis
on
recent
advancements
transistor
(GFET)‐based
biosensors
focuses
four
main
areas:
i)
the
basic
concepts
FETs
specific
GFETs;
ii)
various
state‐of‐the‐art
approaches
to
enhance
performance
GFET‐based
terms
operating
principles
“3S”—stability,
sensitivity,
specificity;
iii)
multiplexed
detection
strategies
biosensors;
iv)
current
challenges
future
perspectives
field
biosensors.
It
is
hoped
that
this
article
can
profoundly
elucidate
development
GFET
inspire
broader
audience.
Abstract
Nano-fabrication
techniques
have
demonstrated
their
vital
importance
in
technological
innovation.
However,
low-throughput,
high-cost
and
intrinsic
resolution
limits
pose
significant
restrictions,
it
is,
therefore,
paramount
to
continue
improving
existing
methods
as
well
developing
new
overcome
these
challenges.
This
is
particularly
applicable
within
the
area
of
biomedical
research,
which
focuses
on
sensing,
increasingly
at
point-of-care,
a
way
improve
patient
outcomes.
Within
this
context,
review
latest
advances
main
emerging
patterning
including
two-photon,
stereo,
electrohydrodynamic,
near-field
electrospinning-assisted,
magneto,
magnetorheological
drawing,
nanoimprint,
capillary
force,
nanosphere,
edge,
nano
transfer
printing
block
copolymer
lithographic
technologies
for
micro-
nanofabrication.
Emerging
enabling
structural
chemical
fabrication
are
categorised
along
with
prospective
physical
techniques.
Established
briefly
outlined
novel
compared
these,
summarising
specific
advantages
shortfalls
alongside
current
lateral
amenability
mass
production,
evaluated
terms
process
scalability
cost.
Particular
attention
drawn
potential
breakthrough
application
areas,
predominantly
studies,
laying
platform
tangible
paths
towards
adoption
alternative
or
combination
established
techniques,
depends
needs
end-user
including,
instance,
tolerance
inherent
limits,
fidelity
reproducibility.
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(30)
Опубликована: Май 23, 2023
Mass
pathogen
screening
is
critical
to
preventing
the
outbreaks
and
spread
of
infectious
diseases.
The
large-scale
epidemic
COVID-19
rapid
mutation
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
virus
have
put
forward
new
requirements
for
detection
identification
techniques.
Here,
we
report
a
CRISPR-based
Amplification-free
Viral
RNA
Electrical
Detection
platform
(CAVRED)
SARS-CoV-2
variants.
A
series
CRISPR
assays
were
designed
amplify
CRISPR-Cas
system's
ability
discriminate
between
mutant
wild
genomes
with
single-nucleotide
difference.
identified
viral
information
was
converted
into
readable
electrical
signals
through
field-effect
transistor
biosensors
achievement
highly
sensitive
single-base
mutations.
CAVRED
can
detect
genome
as
low
1
cp
μL-1
within
20
mins
without
amplification,
this
value
comparable
limit
real-time
quantitative
polymerase
chain
reaction.
Based
on
excellent
ability,
an
8-in-1
array
constructed
realized
40
simulated
throat
swab
samples
variants
95.0
%
accuracy.
advantages
accuracy,
sensitivity,
fast
speed
promise
its
application
in
screening.
Advanced Materials,
Год журнала:
2023,
Номер
35(12)
Опубликована: Янв. 10, 2023
Fast
and
accurate
detection
of
microbial
cells
in
clinical
samples
is
highly
valuable
but
remains
a
challenge.
Here,
simple,
culture-free
diagnostic
system
developed
for
direct
pathogenic
bacteria
water,
urine,
serum
using
an
optical
colorimetric
biosensor.
It
consists
printed
nanoarrays
chemically
conjugated
with
specific
antibodies
that
exhibits
distinct
color
changes
after
capturing
target
pathogens.
By
utilizing
the
internal
capillarity
inside
evaporating
droplet,
preconcentration
achieved
within
few
minutes
to
enable
rapid
identification
more
efficient
bacterial
More
importantly,
scattering
signals
are
significantly
amplified
by
due
strong
near-field
localization,
which
supports
visualizable
analysis
growth,
reproduction,
cell
activity
at
single-cell
level.
Finally,
addition
high
selectivity,
this
nanoarray-based
biosensor
also
capable
quantification
continuous
monitoring
load
on
food
over
broad
linear
range,
limit
10
CFU
mL-1
.
This
work
provides
accessible
user-friendly
tool
point-of-care
testing
pathogens
many
environmental
applications,
possibly
enables
breakthrough
early
prevention
treatment.
Advanced Materials,
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 4, 2023
Promising
advances
in
molecular
medicine
have
promoted
the
urgent
requirement
for
reliable
and
sensitive
diagnostic
tools.
Electronic
biosensing
devices
based
on
field-effect
transistors
(FETs)
exhibit
a
wide
range
of
benefits,
including
rapid
label-free
detection,
high
sensitivity,
easy
operation,
capability
integration,
possessing
significant
potential
application
disease
screening
health
monitoring.
In
this
perspective,
tremendous
efforts
achievements
development
high-performance
FET
biosensors
past
decade
are
summarized,
with
emphasis
interface
engineering
FET-based
electrical
platforms
biomolecule
identification.
First,
an
overview
strategies
modulation
recognition
element
design
is
discussed
detail.
For
further
step,
applications
vitro
detection
real-time
monitoring
biological
systems
comprehensively
reviewed.
Finally,
key
opportunities
challenges
electronic
discussed.
It
anticipated
that
comprehensive
understanding
will
inspire
additional
techniques
developing
highly
sensitive,
specific,
stable
as
well
emerging
designs
next-generation
electronics.
Biosensors,
Год журнала:
2023,
Номер
13(2), С. 226 - 226
Опубликована: Фев. 4, 2023
Currently,
non-communicable
diseases
(NCDs)
have
emerged
as
potential
risks
for
humans
due
to
adopting
a
sedentary
lifestyle
and
inaccurate
diagnoses.
The
early
detection
of
NCDs
using
point-of-care
technologies
significantly
decreases
the
burden
will
be
poised
transform
clinical
intervention
healthcare
provision.
An
imbalance
in
levels
circulating
cell-free
microRNAs
(ccf-miRNA)
has
manifested
NCDs,
which
are
passively
released
into
bloodstream
or
actively
produced
from
cells,
improving
efficacy
disease
screening
providing
enormous
sensing
potential.
effective
ccf-miRNA
continues
significant
technical
challenge,
even
though
sophisticated
equipment
is
needed
analyze
readouts
expression
patterns.
Nanomaterials
come
light
solution
they
provide
advantages
over
other
widely
used
diagnostic
techniques
measure
miRNAs.
Particularly,
CNDs-based
fluorescence
nano-biosensors
great
interest.
Owing
excellent
characteristics
CNDs,
developing
such
sensors
ccf-microRNAs
been
much
more
accessible.
Here,
we
critically
examined
recent
advancements
fluorescence-based
CNDs
biosensors,
including
tools
manufacturing
these
biosensors.
Green
synthesis
methods
scaling
up
high-quality,
fluorescent
natural
source
discussed.
various
surface
modifications
that
help
attach
biomolecules
utilizing
covalent
conjugation
multiple
applications,
self-assembly,
sensing,
imaging,
analyzed.
current
review
particular
interest
researchers
interested
materials
chemistry,
nanomedicine,
related
fields,
focus
on
ccf-miRNAs
applications
medical
field.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(3), С. 1847 - 1855
Опубликована: Янв. 6, 2023
Portable
point-of-care
testing
(POCT)
is
currently
drawing
enormous
attention
owing
to
its
great
potential
for
disease
diagnosis
and
personal
health
management.
Electrochemical
biosensors,
with
the
intrinsic
advantages
of
cost-effectiveness,
fast
response,
ease
miniaturization,
integration,
are
considered
as
one
most
promising
candidates
POCT
application.
However,
clinical
application
electrochemical
biosensors-based
hindered
by
decreased
detection
sensitivity
due
low
abundance
disease-relevant
biomolecules
in
extremely
complex
biological
samples.
Herein,
we
construct
a
flexible
biosensor
based
on
single-stranded
DNA
functionalized
single-walled
carbon
nanotubes
(ssDNA-SWNTs)
high
stability
miRNA-21
human
urine
achieve
bladder
cancer
(BCa)
classification.
The
ssDNA-SWNT
electrodes
2D
interconnected
network
structure
exhibit
electrical
conductivity,
thus
enabling
ultrasensitive
limit
3.0
fM.
Additionally,
flexibility
endows
biosensors
capability
even
under
large
bending
deformations.
In
cohort
40
BCa
patients
at
stages
I–III
44
negative
control
samples,
constructed
could
detect
92.5%
sensitivity,
an
88.6%
specificity,
classify
overall
accuracy
81.0%.
also
be
utilized
treatment
efficiency
assessment
recurrence
monitoring.
Owing
their
excellent
stability,
designed
this
work
propose
strategy
realize
samples
personalized
healthcare.