ACS Catalysis, Год журнала: 2024, Номер 14(21), С. 16392 - 16422
Опубликована: Окт. 23, 2024
Язык: Английский
Small Methods, Год журнала: 2025, Номер unknown
Опубликована: Фев. 2, 2025
RNA-cleaving DNAzymes are in vitro selected functional nucleic acids with inherent catalytic activities. Due to their unique properties, such as high specificity, substrate cleavage capability, and programmability, have emerged powerful tools the fields of analytical chemistry, chemical biology, biomedicine. Nevertheless, biological applications still impeded by several challenges, structural instability, compromised activity environments lack spatiotemporal control designs, which may result false-positive signals, limited efficacy or non-specific activation associated side effects. To address these various strategies been explored regulate DNAzyme through modifications, enhancing stability, selectivity, functionality, thereby positioning them ideal candidates for applications. In this review, a comprehensive overview chemically modified is provided, discussing modification effects modifications on DNAzymes. Specific examples use biosensing gene therapy also presented discussed. Finally, current challenges field addressed offer perspectives potential direction
Язык: Английский
Процитировано
1
ACS Nano, Год журнала: 2023, Номер 17(16), С. 15942 - 15961
Опубликована: Авг. 11, 2023
Orthopedic implants have a high failure rate due to insufficient interfacial osseointegration, especially under osteoporotic conditions. Type H vessels are CD31+EMCN+ capillaries with crucial roles in mediating new bone formation, but their abundance fracture site is highly limited. Herein, we report nanoengineered composite coating improve the situ osseointegration of Ti implant for repair, which realized through inhibiting stimulator interferon genes (STING) endothelial cells (ECs) stimulate type vessel formation. Autonomously catalytic DNAzyme-ZnO nanoflowers (DNFzns) were prepared rolling circle amplification (RCA) STING mRNA-degrading DNAzymes, then integrated on surface and further sequentially complexed thioketal-bridged polydopamine naringenin (Ti/DNFzn/PDA-Nar). ECs mesenchymal stem (MSCs) can be recruited by galvanotaxis, accounting negative charges DNFzn/PDA-Nar, subsequently released Nar reactive oxygen species (ROS) stimulation upregulate nitric oxide synthase (eNOS) ECs, leading enhanced local angiogenesis. Meanwhile, coordinately DNFzns would abolish expression transform newly formed into vessels, thus substantially promoting implants. This study provides application prospects improving osteointegration treatment.
Язык: Английский
Процитировано
15
Analytica Chimica Acta, Год журнала: 2024, Номер 1289, С. 342214 - 342214
Опубликована: Янв. 3, 2024
Язык: Английский
Процитировано
6
Nanoscale, Год журнала: 2024, Номер 16(9), С. 4392 - 4406
Опубликована: Янв. 1, 2024
This review summarizes the amplification effect of SNAs in various combined therapy treatment strategies and highlights recent advances clinical translation.
Язык: Английский
Процитировано
6
Chemical Communications, Год журнала: 2024, Номер 60(78), С. 10805 - 10821
Опубликована: Янв. 1, 2024
DNAzymes, a class of single-stranded catalytic DNA with good stability, high activity, and easy synthesis, functionalization modification properties, have garnered significant interest in the realm biosensing bioimaging. Their integration fluorescent dyes or chemiluminescent moieties has led to remarkable bioimaging outcomes, while DNAzyme-based biosensors demonstrated robust sensitivity selectivity detecting metal ions, nucleic acids, proteins, enzyme activities, exosomes, bacteria microorganisms. In addition, by delivering DNAzymes into tumor cells, mRNA therein can be cleaved regulate expression corresponding which further propelled application cancer gene therapy synergistic therapy. This paper reviews strategies for screening attractive such as SELEX high-throughput sequencing, briefly describes amplification mainly include hairpin assembly (CHA), walker, hybridization chain reaction (HCR), origami, CRISPR-Cas12a, rolling circle (RCA), aptamers. applications bioimaging, biosensing, are also highlighted. Subsequently, possible challenges these practical pointed out, future research directions suggested.
Язык: Английский
Процитировано
6
ACS Nano, Год журнала: 2023, Номер 17(21), С. 22129 - 22144
Опубликована: Ноя. 5, 2023
Pulmonary metastasis of breast cancer is the major cause deaths patients, but effective treatment pulmonary metastases still lacking at present. Herein, a degradable biomimetic DNAzyme biocapsule developed with poly(ethylenimine) (PEI)-DNAzyme complex encapsulated in Mn2+/Zn2+-coordinated inositol hexaphosphate (IP6) capsule modified cRGD targeting peptide for high-efficiency gene therapy both primary and metastatic tumors. This inside acidic lysosomes, leading to release abundant Mn2+/Zn2+ catalytic cleavage EGR-1 mRNA. We find that PEI promotes lysosomal escape released DNAzyme. Both vitro vivo experiments demonstrate apparent downregulation Bcl-2 protein expression after biocapsule, thereby inducing apoptotic death tumor cells. further verify exhibits potent therapeutic efficacy against tumors significant inhibition peri-pulmonary metastasis. study provides promising strategy constructing DNAzyme-based platforms self-supply metal ion cofactors cancer.
Язык: Английский
Процитировано
13
Nucleic Acids Research, Год журнала: 2024, Номер 52(10), С. 5866 - 5879
Опубликована: Апрель 25, 2024
Multivalent recognition and binding of biological molecules is a natural phenomenon that increases the stability (avidity) without decreasing specificity. In this study, we took advantage to increase efficiency maintain high specificity RNA cleavage by DNAzymes (Dz). We designed series DNA constructs containing two Dz agents, named here bivalent devices (BDD). One BDD increased folded fragment up 17-fold in comparison with conventional design. Such an was achieved due both improved probability catalytic cores. By moderating degree agent association BDD, excellent selectivity differentiating single-base mismatched RNA, while maintaining relatively rates. Furthermore, trivalent demonstrated even greater than cleaving RNA. The data suggests cooperative action several RNA-cleaving units can significantly improve cleavage, which important for development Dz-based gene knockdown agents.
Язык: Английский
Процитировано
5
Food Chemistry, Год журнала: 2024, Номер 458, С. 140202 - 140202
Опубликована: Июнь 25, 2024
Язык: Английский
Процитировано
5
Nanoscale, Год журнала: 2023, Номер 15(27), С. 11346 - 11365
Опубликована: Янв. 1, 2023
The review systematically summarizes the applications of RNA-cleaving DNAzymes in recent years, explaining uniqueness and superiority biosensing gene therapy.
Язык: Английский
Процитировано
11
Engineered Regeneration, Год журнала: 2024, Номер 5(2), С. 228 - 246
Опубликована: Апрель 17, 2024
The gut has been a focal point in the research of digestive system disorders. internal microbiota generates metabolites that function as signaling molecules and substrates, interacting with intestinal wall influencing host physiology pathology. Besides, owe highly diverse types quantities, posing challenges for quantitative analysis, monitoring frequent interactions between tract remains challenge. However, targeting elucidated their relevance to diseases. By modulating such short-chain fatty acids, bile lipopolysaccharides, it is possible intervene progression diseases inflammatory bowel disease non-alcoholic liver disease. Currently, on advancing, more work required explore host, microbes underlying mechanisms. In this review, we have revisited generation microbiota-related metabolites, impact diseases, modes interaction, emphasizing significant role It believed linkage current can be established through providing framework foundation field metabolomics fundamental
Язык: Английский
Процитировано
4