ACS Applied Polymer Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 13, 2025
The
treatment
of
severe
bone
defects
remains
an
unresolved
clinical
challenge.
Injectable
hydrogels
loaded
with
drugs
or
growth
factors
are
considered
to
offer
substantial
benefits
in
the
regeneration
irregular
shapes
under
complex
pathological
microenvironments.
In
this
study,
all-silk-derived
composite
hydrogel
was
developed
for
regeneration.
methacrylated
silk
fibroin
(SilMA)
fabricated
situ
injection
and
photocuring
serve
as
a
supporting
matrix,
incorporation
platelet-rich
plasma
(PRP)
promote
migration
pre-differentiation
marrow
mesenchymal
stem
cells
(BMSCs)
during
early
stage,
(SF)
microspheres
encapsulating
berberine
(BBR)
regulate
BMSCs
osteogenesis
over
extended
period.
were
demonstrated
by
upregulating
mitochondrial
biogenesis
fusion.
Additionally,
it
indicated
that
impaired
osteogenic
activity
dynamics
inflammatory
conditions
can
be
reversed
through
addition
SF-BBR
microspheres.
Rat
calvarial
defect
repair
experiments
using
showed
remarkable
increase
local
quantity.
These
findings
suggest
SilMA
compositing
PRP
shows
great
potential
modulating
function.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2023,
Номер
11
Опубликована: Сен. 22, 2023
The
intervertebral
disc
(IVD)
is
a
load-bearing,
avascular
tissue
that
cushions
pressure
and
increases
flexibility
in
the
spine.
Under
influence
of
obesity,
injury,
reduced
nutrient
supply,
it
develops
pathological
changes
such
as
fibular
annulus
(AF)
herniation,
inflammation,
eventually
leading
to
degeneration
(IDD).
Lower
back
pain
(LBP)
caused
by
IDD
severe
chronic
disorder
severely
affects
patients'
quality
life
has
substantial
socioeconomic
impact.
Patients
may
consider
surgical
treatment
after
conservative
failed.
However,
broken
AF
cannot
be
repaired
surgery,
incidence
re-protrusion
reoccurring
high,
possibly
adjacent
vertebrae.
Therefore,
effective
strategies
must
explored
repair
prevent
IDD.
This
paper
systematically
reviews
recent
advances
repairing
IVD,
describes
its
advantages
shortcomings,
explores
future
direction
technology.
Biomaterials Research,
Год журнала:
2025,
Номер
29
Опубликована: Янв. 1, 2025
Repairing
critical
bone
defects
remains
a
formidable
challenge
in
regenerative
medicine.
Scaffolds
that
can
fill
and
facilitate
regeneration
have
garnered
considerable
attention.
However,
scaffolds
struggle
to
provide
an
ideal
microenvironment
for
cell
growth
differentiation
at
the
interior
of
defect
sites.
The
scaffold's
structure
must
meet
specific
requirements
support
endogenous
regeneration.
Here,
we
introduce
novel
3D-printed
nanocolloidal
gelatin
methacryloyl
(GelMA)
hydrogel,
namely,
nG
was
derived
from
self-assembly
GelMA
presence
Pluronics
F68,
emphasizing
its
osteoinductive
capability
conferred
solely
by
structure.
exhibiting
remarkable
pore
connectivity
cell-adaptable
microscopic
structure,
induced
infiltration
migration
rat
mesenchymal
stem
cells
(rBMSCs)
into
hydrogel
with
large
spreading
area
vitro.
Moreover,
interconnected
nanospheres
promoted
osteogenic
rBMSCs,
leading
up-regulated
expression
ALP,
RUNX2,
COL-1,
OCN,
as
well
augmented
formation
calcium
nodules.
In
critical-sized
calvarial
model,
demonstrated
improved
repair
defects,
enhanced
recruitment
CD29+
CD90+
increased
regeneration,
indicated
significantly
higher
mineral
density
(BMD)
vivo.
Mechanistically,
integrin
β1/focal
adhesion
kinase
(FAK)
mechanotransduction
signaling
pathway
group
both
vitro
vivo,
which
may
partially
account
pronounced
capability.
conclusion,
shows
great
potential
near-future
clinical
translational
strategy
customized
defects.
ACS Applied Polymer Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 13, 2025
The
treatment
of
severe
bone
defects
remains
an
unresolved
clinical
challenge.
Injectable
hydrogels
loaded
with
drugs
or
growth
factors
are
considered
to
offer
substantial
benefits
in
the
regeneration
irregular
shapes
under
complex
pathological
microenvironments.
In
this
study,
all-silk-derived
composite
hydrogel
was
developed
for
regeneration.
methacrylated
silk
fibroin
(SilMA)
fabricated
situ
injection
and
photocuring
serve
as
a
supporting
matrix,
incorporation
platelet-rich
plasma
(PRP)
promote
migration
pre-differentiation
marrow
mesenchymal
stem
cells
(BMSCs)
during
early
stage,
(SF)
microspheres
encapsulating
berberine
(BBR)
regulate
BMSCs
osteogenesis
over
extended
period.
were
demonstrated
by
upregulating
mitochondrial
biogenesis
fusion.
Additionally,
it
indicated
that
impaired
osteogenic
activity
dynamics
inflammatory
conditions
can
be
reversed
through
addition
SF-BBR
microspheres.
Rat
calvarial
defect
repair
experiments
using
showed
remarkable
increase
local
quantity.
These
findings
suggest
SilMA
compositing
PRP
shows
great
potential
modulating
function.
