bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 22, 2024
Abstract
Current
challenges
in
tissue
engineering
include
creation
of
extracellular
environments
that
support
and
interact
with
cells
using
biochemical,
mechanical,
structural
cues.
Spatial
control
over
these
cues
is
currently
limited
due
to
a
lack
suitable
fabrication
techniques.
This
study
introduces
Xolography,
an
emerging
dual-color
light-sheet
volumetric
printing
technology,
achieve
mechanical
features
for
hydrogel-based
photoresins
at
micro-to
macroscale
while
within
minutes.
We
propose
water-soluble
photoswitch
photoinitiator
system
are
the
first
demonstrate
Xolography
library
naturally-derived,
synthetic,
thermoresponsive
hydrogels.
Centimeter-scale,
three-dimensional
constructs
positive
20
µm
negative
∼
100
fabricated
properties
(compressive
moduli
0.2
kPa
–
6.5
MPa).
Notably,
switching
from
binary
grayscaled
light
projection
enables
spatial
stiffness
(0.2
16
kPa).
As
proof
concept,
leveraged
hydrogels
introduce
reversible
anisotropic
shape
changes
beyond
isometric
shrinkage.
finally
viable
cell
aggregates,
laying
foundation
cell-laden
dynamic,
cell-instructive
tunable
fast
one-step
process.
Overall,
innovations
unlock
unique
possibilities
across
multiple
biomedical
applications.
Beilstein Journal of Organic Chemistry,
Год журнала:
2025,
Номер
21, С. 242 - 252
Опубликована: Янв. 31, 2025
Aza-diarylethene
has
been
developed
as
a
new
family
of
photochromic
compounds.
This
study
explores
the
properties
and
thermal
back
reactivities
various
aza-diarylethene
regioisomers
(N1-N4
I1-I4)
in
n-hexane.
These
molecules
exhibit
fast
thermally
reversible
reactions
driven
by
6π
aza-electrocyclization.
Kinetic
analysis
reaction
revealed
activation
parameters,
highlighting
how
substitution
position
aryl
group
affects
stability.
Additionally,
density
functional
theory
calculations
identified
M06
MPW1PW91
most
accurate
functionals
for
predicting
reactivity,
closely
matching
experimental
data.
findings
offer
valuable
insights
design
advanced
materials
with
tailored
photophysical
characteristics.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 26, 2025
Abstract
Light‐based
volumetric
bioprinting
enables
fabrication
of
cubic
centimeter‐sized
living
materials
with
micrometer
resolution
in
minutes.
Xolography
is
a
light
sheet‐based
printing
technology
that
offers
unprecedented
generation
rates
and
print
resolutions
for
hard
plastics.
However,
the
limited
solubility
reactivity
current
dual‐color
photoinitiators
(DCPIs)
aqueous
media
have
hindered
their
application
high‐resolution
matter.
Here,
we
present
novel
three‐component
formulation
drastically
improves
photoreactivity
thereby
high‐resolution,
rapid,
cytocompatible
Xolographic
biofabrication
intricately
architected
yet
mechanically
robust
materials.
To
achieve
this,
various
relevant
additives
are
systematically
explored,
which
revealed
diphenyliodonium
chloride
N
‐vinylpyrrolidone
strongly
enhance
D‐mediated
photoreactivity,
as
confirmed
by
photo‐rheology.
This
gelatin
methacryloyl‐based
bioresins,
producing
>1
cm
3
constructs
at
≈20
µm
positive
125
negative
within
Multimaterial
printing,
molecular
patterning,
grayscale‐mediated
mechanical
patterning
explored
to
programmably
create
intricate,
biomimetic,
concentration‐controlled
architectures.
We
demonstrate
Bioxolographic
cell
types,
showing
excellent
viability,
compatibility
long‐term
culture,
ability
nascent
protein
deposition.
These
results
position
Bioxolography
transformative
platform
scalable,
functional
encoded
chemical
properties.
Open Ceramics,
Год журнала:
2024,
Номер
19, С. 100655 - 100655
Опубликована: Авг. 10, 2024
Advanced
ceramics
printed
with
photon-based
additive
manufacturing
deals
anisotropic
mechanical
properties
from
the
layer-by-layer
manufacturing.
Motivated
by
success
in
using
highly
filled
transparent
slurries
containing
nanoparticles
for
powder-based
two-photon-polymerization
(2PP)
advanced
ceramic
printing,
this
works
approach
is
transfer
to
Xolography,
a
volumetric
technology
based
on
linear
two-photon
excitation
and
without
recoating
steps.
This
paper
reports
results
of
preliminary
investigation
optimizing
photocurable
slurry
requirements
Xolography
terms
transparency,
over
significantly
larger
mean
free
path,
compared
2PP.
A
feedstock
70
%
weight
fraction
particles
(∼30
vol%)
exhibiting
an
exceptionally
high
degree
transparency
relevant
wavelength
range
400–800
nm
was
prepared
5
zirconia
nanoparticles.
The
attributed
near-monomodal
particle
size
distribution
used.
Biomacromolecules,
Год журнала:
2024,
Номер
26(1), С. 85 - 117
Опубликована: Дек. 3, 2024
Since
its
invention
in
the
1980s,
photopolymerization-based
3D
printing
has
attracted
significant
attention
for
capability
to
fabricate
complex
microstructures
with
high
precision,
by
leveraging
light
patterning
initiate
polymerization
and
cross-linking
liquid
resin
materials.
Such
precision
makes
it
particularly
suitable
biomedical
applications,
particular,
advanced
customized
drug
delivery
systems.
This
review
summarizes
latest
advancements
photopolymerization
technology
development
of
biocompatible
and/or
biodegradable
materials
that
have
been
used
or
shown
potential
field
delivery.
The
loading
methods
release
characteristics
systems
are
summarized.
Importantly,
recent
trends
applications
based
on
printing,
including
oral
formulations,
microneedles,
implantable
devices,
microrobots
recently
emerging
systems,
analyzed.
In
end,
challenges
opportunities
discussed.
Advanced Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 13, 2024
Abstract
Xolography
is
a
volumetric
3D
printing
technique
utilizing
intersecting
light
beams
within
volume
of
photopolymer
for
spatially
controlled
photopolymerization.
Unlike
layer‐based
methods,
creates
structures
continuously
closed
vat,
eliminating
the
prevalent
need
support
and
allowing
full
geometrical
freedom
at
high
speeds.
The
working
principle
does
not
rely
on
gravity,
making
an
outstanding
technology
additive
manufacturing
under
microgravity
conditions
as
illustrated
in
set
experiments
during
parabolic
flight
campaign.
environment
obviates
rheology
control
resins,
enabling
use
low‐viscosity
formulations
(e.g.,
11
mPa
s)
while
maintaining
fast
precise
acrylic
polymer
resins
hydrogels.
Xolography's
speed
reliability
facilitate
rapid
iterations
print
task
between
Earth's
gravity
conditions.
This
capability
positions
ideal
tool
material
research
space,
offering
significant
cost
efficiency
advantages
over
traditional
methods.
Molecular
photoswitches
produce
light-controlled
changes
at
the
nanometer
scale
and
can
therefore
be
used
to
alter
states
behavior
of
materials
in
a
truly
bottom-up
fashion.
Here
we
show
an
escalating
photonic
complexity
material
property
control
with
light
using
recently
developed
aza-diarylethene
combination
hemiindigo
(HI)
photoswitches.
First,
as
photoswitch
polystyrene
(PS)
inscribe
relief-type
3D
structures
reversibly
into
PS.
Second,
aza-
diarylethene
further
photoinitiator
for
light-induced
polymerization
acrylates,
demonstrating
first
time
chemical
reactivity
its
zwitterionic
switching
state.
Third,
HIs
are
implemented
polymerized
PMA,
generating
photochromic
polymers.
At
fourth
level,
binary
mixture
allows
synergize
induced
photopolymerization
localized
photochromism
simultaneously
entrapped
functional
HI.
With
such
multi-level
response,
utility
this
particular
applications
advanced
is
demonstrated.
