Immobile Integrin Signaling Transit and Relay Nodes Organize Mechanosignaling through Force-Dependent Phosphorylation in Focal Adhesions
ACS Nano,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 6, 2025
Transmembrane
signaling
receptors,
such
as
integrins,
organize
nanoclusters
that
provide
several
advantages,
including
increasing
avidity,
sensitivity
(increasing
the
signal-to-noise
ratio),
and
robustness
(signaling
threshold)
of
signal
in
contrast
to
by
single
receptors.
Furthermore,
compared
large
micron-sized
clusters,
offer
advantage
rapid
turnover
for
disassembly
signal.
However,
whether
function
hubs
remains
poorly
understood.
Here,
we
employ
fluorescence
nanoscopy
combined
with
photoactivation
photobleaching
at
subdiffraction
limited
resolution
∼100
nm
length
scale
within
a
focal
adhesion
examine
dynamics
diverse
proteins.
We
show
(i)
subregions
adhesions
are
enriched
an
immobile
population
integrin
β3
organized
nanoclusters,
which
(ii)
turn
serve
associated
key
adhesome
proteins-vinculin,
kinase
(FAK)
paxillin,
demonstrating
proceeds
formation
rather
than
through
individual
(iii)
Distinct
protein
exhibit
distinct
dynamics,
is
closely
correlated
their
signaling.
(iv)
Long-lived
hubs─wherein
phosphorylated
FAK
form
stable
close
proximity
them,
disassembled
response
inactivation
removal
force
activation
phosphatase
PTPN12.
(v)
Signaling
takes
place
external
signals
or
geometric
arrangement
when
removed,
these
disassemble.
term
functional
transit
relay
nodes
(STARnodes).
Taken
together,
results
demonstrate
STARnodes
seed
downstream
receptors
organizing
proteins
(FAK,
vinculin)
incoming
intracellularly
bring
about
robust
function.
Язык: Английский
Exploring the intersection of mechanobiology and artificial intelligence
npj Biological Physics and Mechanics.,
Год журнала:
2025,
Номер
2(1)
Опубликована: Март 26, 2025
Язык: Английский
A facile nanopattern modification of silk fibroin electrospun scaffold and corresponding impact on cell proliferation and osteogenesis
Regenerative Biomaterials,
Год журнала:
2024,
Номер
11
Опубликована: Янв. 1, 2024
Abstract
As
a
well-known
natural
protein
biomaterial,
silk
fibroin
(SF)
has
shown
broad
application
prospects
in
typical
biomedical
fields.
However,
the
mostly
used
SF
from
Bombyx
mori
silkworm
lacks
specific
cell
adhesion
sites
and
other
bioactive
peptide
sequences,
there
is
still
significant
room
for
further
improvement
of
their
biological
functions.
Therefore,
it
crucial
to
develop
facile
effective
modification
strategy
this
widely
researched
biomaterial.
In
study,
electrospun
scaffold
been
chosen
as
air
plasma
etching
adopted
nanopattern
promote
its
Results
demonstrated
that
could
feasibly
effectively
create
nano-island-like
patterns
on
complex
surface
scaffolds,
detailed
features
be
easily
regulated
by
adjusting
time.
addition,
mesenchymal
stem
responses
have
illustrated
significantly
regulate
corresponding
behaviors.
Compared
with
non-etched
scaffold,
10
min-etched
scaffolds
(10E
scaffold)
promoted
proliferation
osteogenic
differentiation.
Moreover,
10E
also
confirmed
accelerate
vascularization
ectopic
osteogenesis
vivo
using
rat
subcutaneous
implantation
model.
mentioned
promoting
effects
would
weakened
or
even
counteracted
increase
conclusion,
great
potential
Thus,
provided
useful
guidance
excellent
SF-based
suitable
bone
tissue
engineering.
Язык: Английский
Ternary Heteronanocrystals with Dual-Heterojunction for Boosting Near-Infrared-Triggered Photo-Chemodynamic Therapy
Journal of the American Chemical Society,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 12, 2024
Strongly
coupled
interfaces
in
the
epitaxial
growth
heteronanocrystals
(HNCs)
provide
advanced
functionalities
regarding
interface
connection,
electron
transfer,
and
carrier
separation.
However,
majority
of
current
nanocomposites
primarily
focus
on
a
single
heterojunction
involving
only
two
subunits,
which
hinders
achievement
optimized
synergy
energy
transfer
among
more
than
components.
Herein,
ternary
NaGdF
Язык: Английский
Ligand Inter‐Relation Analysis Via Graph Theory Predicts Macrophage Response
Advanced Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 24, 2024
Abstract
Graph
theory
has
been
widely
used
to
quantitatively
analyze
complex
networks
of
molecules,
materials,
and
cells.
Analyzing
the
dynamic
structure
extracellular
matrix
can
predict
cell‐material
interactions
but
not
yet
demonstrated.
In
this
study,
graph
theory‐based
mathematical
modeling
RGD
ligand
inter‐relation
is
demonstrated
by
differentially
cutting
off
RGD‐to‐RGD
interlinkages
with
flexibly
conjugated
magnetic
nanobars
(MNBs)
tunable
aspect
ratio.
The
are
less
effectively
cut
MNBs
a
lower
ratio,
which
decreases
shortest
path
while
increasing
number
instances
thereof,
thereby
augmenting
nano
inter‐relation.
This
facilitates
integrin
recruitment
macrophages
thus
actin
fiber
assembly
vinculin
expression,
mediates
pro‐regenerative
polarization,
involving
myosin
II,
polymerization,
rho‐associated
protein
kinase.
Unidirectional
pre‐aligning
or
reversibly
lifting
highly
elongated
both
increase
inter‐relation,
promotes
host
macrophage
adhesion
switches
their
polarization
from
pro‐inflammatory
phenotype.
latter
approach
produces
nano‐spaces
through
penetrate
establish
links
thereunder.
Using
theory,
study
presents
example
mathematically
functionality
extracellular‐matrix‐mimetic
help
elucidate
dynamics
occurring
between
cells
materials
via
versatile
geometrical
nano‐engineering.
Язык: Английский