Engineered Living Systems Based on Gelatin: Design, Manufacturing, and Applications
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 5, 2025
Engineered
living
systems
(ELSs)
represent
purpose-driven
assemblies
of
components,
encompassing
cells,
biomaterials,
and
active
agents,
intricately
designed
to
fulfill
diverse
biomedical
applications.
Gelatin
its
derivatives
have
been
used
extensively
in
ELSs
owing
their
mature
translational
pathways,
favorable
biological
properties,
adjustable
physicochemical
characteristics.
This
review
explores
the
intersection
gelatin
with
fabrication
techniques,
offering
a
comprehensive
examination
synergistic
potential
creating
for
various
applications
biomedicine.
It
offers
deep
dive
into
gelatin,
including
structures
production,
sources,
processing,
properties.
Additionally,
techniques
employing
derivatives,
generic
microfluidics,
3D
printing
methods.
Furthermore,
it
discusses
based
on
regenerative
engineering
as
well
cell
therapies,
bioadhesives,
biorobots,
biosensors.
Future
directions
challenges
are
also
examined,
highlighting
emerging
trends
areas
improvements
innovations.
In
summary,
this
underscores
significance
gelatin-based
advancing
lays
groundwork
guiding
future
research
developments
within
field.
Язык: Английский
Bioprinting functional hepatocyte organoids derived from human chemically induced pluripotent stem cells to treat liver failure
Gut,
Год журнала:
2025,
Номер
unknown, С. gutjnl - 333885
Опубликована: Март 3, 2025
Background
To
treat
liver
failure,
three-dimensional
(3D)
bioprinting
is
a
promising
technology
used
to
construct
hepatic
tissue
models.
However,
current
research
on
of
models
primarily
relies
conventional
single-cell-based
bioprinting,
where
individual
functional
hepatocytes
are
dispersed
and
isolated
within
hydrogels,
leading
insufficient
treatment
outcomes
due
inadequate
cell
functionality.
Objective
Here,
we
aim
bioprint
model
using
hepatocyte
organoids
(HOs)
evaluate
its
liver-specific
functions
in
vitro
vivo
.
Design
Human
chemically
induced
pluripotent
stem
cells
(hCiPSCs)
were
as
robust
non-genome-integrative
source
produce
highly
viable
HOs
(hCiPSC-HOs).
An
oxygen-permeable
microwell
device
was
enhance
oxygen
supply,
ensuring
high
viability
promoting
hCiPSC-HOs
maturation.
maintain
the
long-term
biofunction
hCiPSC-HOs,
spheroid-based
employed
(3DP-HOs).
3DP-HOs
intraperitoneally
implanted
mice
with
failure.
Results
demonstrated
enhanced
when
compared
fabricated
exhibited
gene
profiles
closely
resembling
while
maintaining
Moreover,
implantation
significantly
improved
survival
CCl
4
-induced
acute-on-chronic
failure
also
Fah−/−
reduced
injury,
inflammation
fibrosis
indices
regeneration
expression.
Conclusion
Our
bioprinted
exhibits
remarkable
therapeutic
efficacy
for
holds
great
potential
clinical
field
regenerative
medicine.
Язык: Английский
Designer mammalian living materials through genetic engineering
Bioactive Materials,
Год журнала:
2025,
Номер
48, С. 135 - 148
Опубликована: Фев. 15, 2025
Язык: Английский
3D printing for tissue/organ regeneration in China
Bio-Design and Manufacturing,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 5, 2025
Язык: Английский
3D printing of bioactive-loaded electrospun/electrosprayed structures
Elsevier eBooks,
Год журнала:
2025,
Номер
unknown, С. 343 - 358
Опубликована: Янв. 1, 2025
Язык: Английский
Embedded Bioprinting of Tumor-Scale Pancreatic Cancer-Stroma 3D Models for Preclinical Drug Screening
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(42), С. 56718 - 56729
Опубликована: Окт. 10, 2024
The
establishment
of
organotypic
preclinical
models
that
accurately
resemble
the
native
tumor
microenvironment
at
an
anatomic
human
scale
is
highly
desirable
to
level
up
in
vitro
platforms
potential
for
screening
candidate
therapies.
bioengineering
anatomic-scaled
three-dimensional
(3D)
emulate
while
recapitulating
their
cellular
and
matrix
components
remains,
however,
be
fully
realized.
In
this
focus,
herein,
we
leveraged
embedded
3D
bioprinting
biofabricating
pancreatic
ductal
adenocarcinoma
(PDAC)
combining
gelatin-methacryloyl
hyaluronic
acid
methacrylate
extracellular
(ECM)-mimetic
biomaterials
with
cancer
cells
cancer-associated
fibroblasts
generate
capable
emulating
size
(∼6
mm)
stromal
elements.
By
using
a
viscoelastic
continuous
polymeric
supporting
bath,
tumor-scale
were
rapidly
generated
(∼50
constructs/h)
easily
recovered
following
in-bath
visible
light
photocrosslinking.
As
proof-of-concept,
tissue-scale
constructs
displaying
physiomimetic
designs
biofabricated.
These
also
encompass
incorporation
compartment
better
PDAC
(TME)
its
stratified
spatial
organization.
Cell-laden
tumor-size
remained
viable
14
days
responsive
Gemcitabine
dose-dependent
mode.
Cancer-stroma
exhibited
increased
drug
resistance
compared
monotypic
counterparts,
highlighting
key
role
chemotherapeutic
resistance.
Overall,
report
first
time
freeform
biofabrication
exhibiting
scale,
different
structural
complexities,
engineered
cancer-stromal
compartments,
being
valuable
therapeutics.
Язык: Английский
3D Bioprinting of Liquid High‐Cell‐Proportion Bioinks in Liquid Granular Bath
Advanced Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 10, 2024
Embedded
3D
bioprinting
techniques
have
emerged
as
a
powerful
method
to
fabricate
engineered
constructs
using
low
strength
bioinks;
however,
there
are
challenges
in
simultaneously
satisfying
the
requirements
of
high-cell-activity,
high-cell-proportion,
and
low-viscosity
bioinks.
In
particular,
printing
capacity
embedded
is
limited
two
main
challenges:
spreading
diffusion,
especially
for
liquid,
high-cell-activity
bioinks
that
can
facilitate
high-cell-proportion.
Here,
liquid-in-liquid
(LL3DBP)
strategy
developed,
which
used
liquid
granular
bath
prevent
during
printing,
electrostatic
interaction
between
baths
found
effectively
diffusion
As
an
example,
positively
charged
5%
w/v
gelatin
methacryloyl
(GelMA)
prepared
with
negatively
κ-carrageenan
proved
be
achievable.
By
LL3DBP,
greatly
advanced
over
90%
v/v
cell
printed,
printed
structures
high-cell-proportion
exhibit
excellent
bioactivity.
Язык: Английский