3D Bioprinting of Liquid High‐Cell‐Proportion Bioinks in Liquid Granular Bath DOI Open Access

Jinhong Jiang,

Chenhui Yuan,

Xinyu Zhang

и другие.

Advanced Materials, Год журнала: 2024, Номер unknown

Опубликована: Окт. 10, 2024

Embedded 3D bioprinting techniques have emerged as a powerful method to fabricate engineered constructs using low strength bioinks; however, there are challenges in simultaneously satisfying the requirements of high-cell-activity, high-cell-proportion, and low-viscosity bioinks. In particular, printing capacity embedded is limited two main challenges: spreading diffusion, especially for liquid, high-cell-activity bioinks that can facilitate high-cell-proportion. Here, liquid-in-liquid (LL3DBP) strategy developed, which used liquid granular bath prevent during printing, electrostatic interaction between baths found effectively diffusion As an example, positively charged 5% w/v gelatin methacryloyl (GelMA) prepared with negatively κ-carrageenan proved be achievable. By LL3DBP, greatly advanced over 90% v/v cell printed, printed structures high-cell-proportion exhibit excellent bioactivity.

Язык: Английский

Engineered Living Systems Based on Gelatin: Design, Manufacturing, and Applications DOI Open Access
Zhenwu Wang, Lin Zeng, Xuan Mei

и другие.

Advanced Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

Engineered living systems (ELSs) represent purpose-driven assemblies of components, encompassing cells, biomaterials, and active agents, intricately designed to fulfill diverse biomedical applications. Gelatin its derivatives have been used extensively in ELSs owing their mature translational pathways, favorable biological properties, adjustable physicochemical characteristics. This review explores the intersection gelatin with fabrication techniques, offering a comprehensive examination synergistic potential creating for various applications biomedicine. It offers deep dive into gelatin, including structures production, sources, processing, properties. Additionally, techniques employing derivatives, generic microfluidics, 3D printing methods. Furthermore, it discusses based on regenerative engineering as well cell therapies, bioadhesives, biorobots, biosensors. Future directions challenges are also examined, highlighting emerging trends areas improvements innovations. In summary, this underscores significance gelatin-based advancing lays groundwork guiding future research developments within field.

Язык: Английский

Процитировано

3
Bioprinting functional hepatocyte organoids derived from human chemically induced pluripotent stem cells to treat liver failure DOI

Guangya Li,

Jianyu He,

Jihang Shi

и другие.

Gut, Год журнала: 2025, Номер unknown, С. gutjnl - 333885

Опубликована: Март 3, 2025

Background To treat liver failure, three-dimensional (3D) bioprinting is a promising technology used to construct hepatic tissue models. However, current research on of models primarily relies conventional single-cell-based bioprinting, where individual functional hepatocytes are dispersed and isolated within hydrogels, leading insufficient treatment outcomes due inadequate cell functionality. Objective Here, we aim bioprint model using hepatocyte organoids (HOs) evaluate its liver-specific functions in vitro vivo . Design Human chemically induced pluripotent stem cells (hCiPSCs) were as robust non-genome-integrative source produce highly viable HOs (hCiPSC-HOs). An oxygen-permeable microwell device was enhance oxygen supply, ensuring high viability promoting hCiPSC-HOs maturation. maintain the long-term biofunction hCiPSC-HOs, spheroid-based employed (3DP-HOs). 3DP-HOs intraperitoneally implanted mice with failure. Results demonstrated enhanced when compared fabricated exhibited gene profiles closely resembling while maintaining Moreover, implantation significantly improved survival CCl 4 -induced acute-on-chronic failure also Fah−/− reduced injury, inflammation fibrosis indices regeneration expression. Conclusion Our bioprinted exhibits remarkable therapeutic efficacy for holds great potential clinical field regenerative medicine.

Язык: Английский

Процитировано

2
Designer mammalian living materials through genetic engineering DOI
Mariana Gameiro, J. Almeida-Pinto, Beatriz S. Moura

и другие.

Bioactive Materials, Год журнала: 2025, Номер 48, С. 135 - 148

Опубликована: Фев. 15, 2025

Язык: Английский

Процитировано

0
3D printing for tissue/organ regeneration in China DOI Creative Commons
Chaofan He, Jiankang He, Chengtie Wu

и другие.

Bio-Design and Manufacturing, Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Язык: Английский

Процитировано

0
3D printing of bioactive-loaded electrospun/electrosprayed structures DOI
Loleny Tavares, Jorge Luís, Lúcia Santos

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 343 - 358

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Embedded Bioprinting of Tumor-Scale Pancreatic Cancer-Stroma 3D Models for Preclinical Drug Screening DOI
Maria V. Monteiro, Marta Rocha, Mariana T. Carvalho

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(42), С. 56718 - 56729

Опубликована: Окт. 10, 2024

The establishment of organotypic preclinical models that accurately resemble the native tumor microenvironment at an anatomic human scale is highly desirable to level up in vitro platforms potential for screening candidate therapies. bioengineering anatomic-scaled three-dimensional (3D) emulate while recapitulating their cellular and matrix components remains, however, be fully realized. In this focus, herein, we leveraged embedded 3D bioprinting biofabricating pancreatic ductal adenocarcinoma (PDAC) combining gelatin-methacryloyl hyaluronic acid methacrylate extracellular (ECM)-mimetic biomaterials with cancer cells cancer-associated fibroblasts generate capable emulating size (∼6 mm) stromal elements. By using a viscoelastic continuous polymeric supporting bath, tumor-scale were rapidly generated (∼50 constructs/h) easily recovered following in-bath visible light photocrosslinking. As proof-of-concept, tissue-scale constructs displaying physiomimetic designs biofabricated. These also encompass incorporation compartment better PDAC (TME) its stratified spatial organization. Cell-laden tumor-size remained viable 14 days responsive Gemcitabine dose-dependent mode. Cancer-stroma exhibited increased drug resistance compared monotypic counterparts, highlighting key role chemotherapeutic resistance. Overall, report first time freeform biofabrication exhibiting scale, different structural complexities, engineered cancer-stromal compartments, being valuable therapeutics.

Язык: Английский

Процитировано

2
3D Bioprinting of Liquid High‐Cell‐Proportion Bioinks in Liquid Granular Bath DOI Open Access

Jinhong Jiang,

Chenhui Yuan,

Xinyu Zhang

и другие.

Advanced Materials, Год журнала: 2024, Номер unknown

Опубликована: Окт. 10, 2024

Embedded 3D bioprinting techniques have emerged as a powerful method to fabricate engineered constructs using low strength bioinks; however, there are challenges in simultaneously satisfying the requirements of high-cell-activity, high-cell-proportion, and low-viscosity bioinks. In particular, printing capacity embedded is limited two main challenges: spreading diffusion, especially for liquid, high-cell-activity bioinks that can facilitate high-cell-proportion. Here, liquid-in-liquid (LL3DBP) strategy developed, which used liquid granular bath prevent during printing, electrostatic interaction between baths found effectively diffusion As an example, positively charged 5% w/v gelatin methacryloyl (GelMA) prepared with negatively κ-carrageenan proved be achievable. By LL3DBP, greatly advanced over 90% v/v cell printed, printed structures high-cell-proportion exhibit excellent bioactivity.

Язык: Английский

Процитировано

2