DNA Nanomaterial-Based Electrochemical Biosensors for Clinical Diagnosis
ACS Nano,
Год журнала:
2024,
Номер
18(46), С. 31713 - 31736
Опубликована: Ноя. 7, 2024
Sensitive
and
quantitative
detection
of
chemical
biological
molecules
for
screening,
diagnosis
monitoring
diseases
is
essential
to
treatment
planning
response
monitoring.
Electrochemical
biosensors
are
fast,
sensitive,
easy
miniaturize,
which
has
led
rapid
development
in
clinical
diagnosis.
Benefiting
from
their
excellent
molecular
recognition
ability
high
programmability,
DNA
nanomaterials
could
overcome
the
Debye
length
electrochemical
by
simple
design
well
suited
as
elements
biosensors.
Therefore,
enhance
sensitivity
specificity
biosensors,
significant
progress
been
made
recent
years
optimizing
design.
Here,
establishment
sensing
strategies
based
on
reviewed
detail.
First,
structural
nanomaterial
examined
improving
overcoming
length.
In
addition,
electrical
signal
transduction
amplification
reviewed,
applications
nanomaterial-based
integrated
devices
further
summarized.
Finally,
main
opportunities
challenges
detecting
disease
biomarkers
presented
an
aim
guide
with
specificity.
Язык: Английский
An epidermal serine sensing system for skin healthcare
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Март 18, 2025
Portable
biosensors
mainly
focus
on
detecting
biomarkers
in
biofluids
but
neglect
the
abundant
skin
stratum
corneum,
which
are
associated
with
functionality
and
integrity
of
barrier.
Here,
we
propose
a
sensing
patch
designed
for
direct
sampling
situ
quantification
epidermal
serine,
an
important
biomarker
healthcare.
The
consists
porous
hydrogel
serine
diffusion
ion
conduction,
molecular
imprinted
polymer-based
electrochemical
sensor.
By
integrating
customized
handheld
tester,
system
enables
measurement
levels.
We
demonstrate
application
this
assessing
moisturizing
effect
skincare
product
tracking
recovery
progress
barrier
function
patient
atopic
dermatitis.
Our
work
opens
up
potential
scenario
portable
personalized
Serine
is
authors
introduce
capable
Язык: Английский
DNA Framework-Enabled Ocular Barrier Penetration for Microinvasive Antiangiogenic Therapy
Journal of the American Chemical Society,
Год журнала:
2025,
Номер
147(9), С. 7545 - 7554
Опубликована: Фев. 20, 2025
Therapeutic
aptamers
targeting
vascular
endothelial
growth
factor
A
(VEGFA)
have
advanced
the
development
of
antiangiogenic
drugs
for
treating
choroidal
neovascularization
(CNV)
diseases.
However,
despite
FDA
approval
use
in
neovascular
age-related
macular
degeneration
(nAMD),
effective
vivo
delivery
therapeutic
is
hindered
by
ocular
barriers
and
rapid
degradation
biofluids.
Here,
we
demonstrated
a
microinvasive
VEGFA-targeted
to
fundus
using
tetrahedral
framework
nucleic
acids
(tFNAs).
Upon
incorporating
anti-VEGFA
tFNAs
(apt-tFNA),
interrogated
their
penetration
across
outer
blood-retinal
barrier
(oBRB)
innermost
retinal
eyeball,
while
maintaining
structural
integrity.
In
addition,
apt-tFNA
showed
superior
efficacy
inhibiting
proliferation
migration
neutralizing
VEGFA.
Furthermore,
laser-induced
CNV
mouse
model,
subconjunctival
injection
exhibited
comparable
intravitreal
ranibizumab,
monoclonal
antibody
fragment.
These
findings
suggest
that
FNAs
can
effectively
deliver
without
compromising
properties,
highlighting
potential
feasible
periocular
administration
ophthalmic
Язык: Английский
A highly sensitive electrochemical aptasensor for detecting broad-spectrum estrogen molecules in clinical samples
Talanta,
Год журнала:
2024,
Номер
283, С. 127071 - 127071
Опубликована: Окт. 23, 2024
Язык: Английский
Hierarchical DNA Octahedral Nanoplatform for in Situ Biosensing and Clinical Monitoring of Acute Lymphoblastic Leukemia
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 29, 2025
Abstract
Developing
nanoscale
platforms
with
high
integration,
assembly
efficiency,
and
structural
stability
for
performing
complex
computations
in
specific
cells
remains
a
significant
challenge.
To
address
this,
the
Three‐dimensional
Hierarchical
Octahedral
Robotic
(THOR)
DNA
nanoplatform
is
introduced,
which
integrates
targeting,
logic
computation,
sensing
modules
within
single
framework.
This
specifically
binds
to
cancer
cell
surface
proteins,
releasing
aptamer‐linked
fuel
chains
initiate
subsequent
computational
processes.
Three
gates
efficiently
compute
any
arbitrary
binary
combination
of
target
proteins.
The
module
employs
catalytic
hairpin
detecting
miRNAs
sensitivity.
THOR
demonstrates
robust
functionality
both
vitro
situ.
As
proof‐of‐concept,
this
distinguish
acute
lymphoblastic
leukemia
(ALL)
patients
from
other
subtypes
healthy
participants,
achieving
100%
accuracy
applied.
Additionally,
approach
reliably
monitored
therapeutic
progress
ALL
patients,
showing
strong
concordance
bone
marrow
smear
results.
platform
highlights
feasibility
constructing
reliable,
hierarchical,
multifunctional
analytical
system
based
on
polyhedron.
It
offers
promising
auxiliary
tool
clinical
diagnostics
monitoring.
Язык: Английский
Microfluidic magnetic droplet-based chemiluminescence enzyme immunoassay for multiplex sepsis biomarker screening
The Analyst,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
A
chemiluminescence
enzyme
immunoassay
based
on
microfluidic
magnetic
droplets
for
multiplex
sepsis
biomarker
screening,
which
has
great
potential
broader
applications
in
biomolecule
detection
and
early
disease
diagnosis,
is
reported.
Язык: Английский
S9.6 Antibody-Mediated Wireless Portable Biosensor with Multiple Affinity Enhancements for Comprehensive Detection of Nucleic Acid in Serum
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 22, 2025
Creating
biosensors
capable
of
facilely
and
entirely
excluding
the
influence
interfering
biomolecules
in
complex
samples
holds
profound
significance
for
advancing
detection
technology
diagnostics.
Here,
we
develop
a
wireless
portable
biosensor
(WPB)
that
prevents
interference
from
abundant
serum
through
homogeneous
hybridization
S9.6
antibody-mediated
multivalent
capture.
By
transferring
environment
heterogeneous
chip
surface
to
solution,
maintains
consistent
efficiency
as
buffer.
Additionally,
use
capture
ensures
nearly
unchanged
binding
affinity
compared
On
basis
multiple
enhancements,
WPB
can
achieve
ultrasensitive
nucleic
acid
50%
human
serum.
Specifically,
subtle
blocker
is
designed
eliminate
competitive
monovalent
antibody-heteroduplex
binding,
ensuring
interactions.
The
also
enables
single-step
detection.
Moreover,
sensing
platform
utilizes
interferents
situ
antifouling
prevent
nonspecific
adsorption.
As
result,
proposed
achieves
similar
limit
acids
(95
aM)
buffer
(86
aM).
This
approach
inspires
new
idea
elimination
usage
exhibits
comprehensive
performance
with
potential
future
diagnostic
applications.
Язык: Английский