ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
17(1), С. 197 - 210
Опубликована: Дек. 24, 2024
Effective
repair
of
large
bone
defects
through
tissue
engineering
(BTE)
remains
an
unmet
clinical
challenge.
Successful
BTE
requires
optimal
and
synergistic
interactions
among
biocompatible
scaffolds,
osteogenic
factors,
osteoprogenitors
to
form
a
highly
vascularized
microenvironment
for
regeneration
osseointegration.
We
sought
develop
effective
system
by
using
3D
printed
citrate-based
mPOC/hydroxyapatite
(HA)
composites
laden
with
BMP9-stimulated
human
urine
stem
cells
(USCs).
Specifically,
we
synthesized
characterized
methacrylate
poly(1,8
octamethylene
citrate)
(mPOC),
mixed
it
0%,
40%
or
60%
HA
(i.e.,
mPOC-0HA,
mPOC-40HA,
mPOC-60HA),
fabricated
composite
scaffold
via
micro-continuous
liquid
interface
production
(μCLIP).
The
3D-printed
mPOC-HA
scaffolds
were
compatible
USCs
that
exhibited
high
activity
in
vitro
upon
BMP9
stimulation.
Subcutaneous
implantation
revealed
formation
all
three
types
scaffolds.
Histologic
evaluation
the
mPOC-60HA
yielded
most
mature
bone,
resembling
native
extensive
scaffold-osteointegration.
Collectively,
these
findings
demonstrate
composite,
cells,
potent
factor
constitute
desirable
triad
engineering.
Bioactive Materials,
Год журнала:
2024,
Номер
41, С. 427 - 439
Опубликована: Авг. 1, 2024
Approaches
to
regenerating
bone
often
rely
on
integrating
biomaterials
and
biological
signals
in
the
form
of
cells
or
cytokines.
However,
from
a
translational
point
view,
these
approaches
are
challenging
due
sourcing
quality
biologic,
unpredictable
immune
responses,
complex
regulatory
paths,
high
costs.
We
describe
simple
manufacturing
process
material-centric
3D-printed
composite
scaffold
system
(CSS)
that
offers
distinct
advantages
for
clinical
translation.
The
CSS
comprises
porous
polydiolcitrate-hydroxyapatite
elastomer
infused
with
polydiolcitrate-graphene
oxide
hydrogel
composite.
Using
micro-continuous
liquid
interface
production
3D
printer,
we
fabricate
precise
ceramic
60
wt%
hydroxyapatite
resembling
natural
bone.
resulting
integrates
thermoresponsive
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 22, 2025
Abstract
The
rapid
and
efficient
bone
regeneration
is
still
in
unsatisfactory
outcomes,
demonstrating
alternative
strategy
molecular
mechanism
necessary.
Nanoscale
biomaterials
have
shown
some
promising
results
enhancing
regeneration,
however,
the
detailed
interaction
between
nanomaterial
cells/tissue
formation
not
clear.
Herein,
a
molecular‐based
inorganic–organic
poly(citrate‐siloxane)
(PCS)
reported
which
can
rapidly
enhance
osteogenic
differentiation
through
special
with
cellular
surface
communication
network
factor
3
(CCN3),
further
activating
Wnt10b/β‐catenin
signaling
pathway.
Further
studies
revealed
that
CCN3
key
bridge
protein
for
transmitting
osteoinductive
effects
of
nano
PCS
into
intracellular
compartment
Wnt10b.
Specifically,
confirmed
inorganic
silicon
hydroxyl
organic
ester
group
bound
to
Thrombospondin‐1
(TSP‐1)
von
Willebrand
type
C
repeat
module
(vWC)
structural
domains
respectively.
material‐protein
induced
conformational
change
activated
function
TSP‐1
domain,
associated
binding
activation
Wnt10b
signaling.
This
study
reveals
first
targets
nanobiomaterials
promote
tissue
interactions
provides
new
ideas
development
materiobiology.
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 7, 2025
Intervertebral
disc
(IVD)
degeneration
(IVDD),
primarily
caused
by
nucleus
pulposus
(NP)
dehydration,
leads
to
low
back
pain.
While
current
treatments
focus
on
symptom
management
or
surgical
intervention,
tissue
engineering
using
IVD-derived
cells,
biofactors,
and
scaffolds
offers
a
promising
regenerative
approach.
Here,
human
NP
cells
(NPCs)
annulus
fibrosus
(AFCs)
are
immortalized
with
telomerase
reverse
transcriptase
(hTERT),
generating
NPCs
(iHNPCs)
AFCs
(iHAFCs).
These
express
AF-specific
markers,
reversible
via
FLP
recombinase,
non-tumorigenic.
iHAFCs
exhibit
osteogenic
potential,
while
iHNPCs
show
chondrogenic
differentiation.
A
3D-printed
citrate-based
scaffold
was
employed
develop
an
IVD
regeneration
model,
BMP9-stimulated
in
the
peripheral
region
BMP2-stimulated
central
region.
Histological
analysis
revealed
bone
formation
iHAFC
cartilage
iHNPC
region,
mimicking
natural
structure.
Additionally,
ex
vivo
spine
fusion
model
demonstrated
robust
iHAFC-treated
segments.
findings
highlight
potential
of
as
valuable
tools
for
regeneration.
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 2, 2025
Periodontal
bone
defect
(PBD)
treatment
involving
oral
soft
and
hard
tissues
is
complicated
high
requirements
for
regenerated
materials.
Besides
osteogenic
effects,
the
materials
are
also
required
to
have
function
of
barrier
promote
wound
healing.
Citrate
reported
formation
through
enhanced
osteoinductivity
facilitate
healing
by
enabling
phased
angiogenesis.
Herein,
a
novel
tri-layered
citrate-based
hydroxyapatite
(Ci-HA)
composite
scaffold
that
serves
as
substitute
developed
"one-pot"
method
PBD
treatment.
It
found
Ci-HA
degradation
products
can
osteoblastic
differentiation
rat
mesenchymal
stem
cells
human
periodontal
ligament
upregulate
angiogenesis-related
gene
expression
in
gingival
fibroblasts.
Moreover,
3D
remodeling
vitro
shows
an
intermediate
layer
acts
optimal
barrier.
In
vivo
evaluation
intrabony
three-wall
model
significantly
increased
with
markedly
osseointegration
better
properties
similarly
commercial
Bio-Oss
powder
Bio-Gide
membrane.
Thus,
biocompatibility
may
represent
promising
biomaterial
multi-effective
regeneration,
effect,
capacity
PBD.
Advanced Materials Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 15, 2025
Abstract
Infectious
bone
defects
represent
a
significant
clinical
challenge,
characterized
by
prolonged
treatment
durations,
high
medical
costs,
propensity
for
recurrence,
and
risk
of
disability.
This
issue
remains
critical
concern
in
the
orthopedic
field
globally.
The
successful
management
infectious
hinges
on
two
primary
aspects:
reconstruction
eradication
infection.
Synthetic
repair
materials
can
be
broadly
categorized
into
metallic
materials,
inorganic
compounds,
organic
polymers,
composite
materials.
While
antibiotics
address
most
bacterial
infections,
their
efficacy
treating
infections
is
limited
due
to
unique
physiological
anatomical
characteristics
tissue.
Consequently,
there
an
urgent
need
develop
localized,
non‐antibiotic
antibacterial
strategies.
Incorporating
components
help
establish
antimicrobial
microenvironment
at
defect
sites,
promoting
effective
regeneration.
Currently,
are
frequently
combined
with
organic,
inorganic,
stimuli‐responsive,
multi‐modal
agents.
review
aims
provide
overview
commonly
used
integration
agents,
offering
insights
guide
development
next‐generation
thereby
addressing
needs
practice.
