Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 25, 2024
Bacterial
infections
resistant
to
antimicrobial
treatments,
particularly
those
caused
by
Pseudomonas
aeruginosa
(P.
aeruginosa),
frequently
lead
elevated
mortality
rates.
Tackling
this
resistance
using
therapeutic
combinations
with
varied
mechanisms
has
shown
considerable
promise.
In
study,
a
bioinspired
nanocarrier
is
successfully
designed
and
engineered
for
targeted
antibiotic
delivery
toxin/bacteria
clearance.
This
achieved
encapsulating
antibiotic-loaded
framework
nucleic
acids
hybrid
cell
membranes
acquired
from
neutrophils
platelets.
By
coating
the
membrane
outside
shell,
nanocarriers
are
endowed
function
of
neutrophil-like
chemotaxis
platelet-like
bacteria
adhesion
achieve
first
stage
inflammation
targeting.
Based
on
specific
binding
toxin
membrane,
release
triggered
toxin-mediated
lysis
fulfill
second
neutralization
killing.
Meanwhile,
immunomodulation
potential
enables
accomplish
third
reversing
immunosuppressive
microenvironment.
mouse
models
acute
chronic
P.
pneumonia,
can
reduce
bacterial
burden
at
low
dosage
decrease
negligible
toxicity.
sum,
these
findings
have
illustrated
remarkable
capability
in
treating
recalcitrant
infections.
JACS Au,
Год журнала:
2025,
Номер
5(2), С. 486 - 520
Опубликована: Фев. 10, 2025
Tetrahedral
framework
nucleic
acids
(tFNAs)
represent
a
promising
advancement
in
acid
nanotechnology
due
to
their
unique
structural
properties,
high
biocompatibility,
and
multifaceted
biomedical
applications.
Constructed
through
one-pot
annealing
method,
four
single-stranded
DNAs
self-assemble
into
stable,
three-dimensional
tetrahedral
nanostructures
with
enhanced
mechanical
robustness
physiological
stability,
resisting
enzymatic
degradation.
Their
ability
permeate
mammalian
cells
without
transfection
agents,
coupled
modifiable
surfaces,
positions
tFNAs
as
versatile
carriers
for
drug
gene
delivery
systems.
The
tFNA-based
platforms
exhibit
superior
therapeutic
efficacy,
including
antioxidative
anti-inflammatory
effects,
alongside
efficient
cellular
uptake
tissue
penetration.
These
features
underpin
role
precision
medicine,
enabling
targeted
of
diverse
agents
such
synthetic
compounds,
peptides,
acids.
Additionally,
demonstrate
significant
potential
regenerative
immune
modulation,
antibacterial
strategies,
oncology.
By
addressing
challenges
translational
integration,
stand
poised
accelerate
the
development
research
clinical
applications,
fostering
novel
therapies
enhancing
outcomes
across
wide
spectrum
diseases.
This
Perspective
thoroughly
details
attributes
applications
critically
evaluates
tFNAs'
potential,
outlining
inherent
implementation
exploring
solutions
these
obstacles.
Abstract
Colorectal
cancer
(CRC)
remains
a
significant
global
health
challenge,
underscoring
the
need
for
innovative
therapeutic
strategies.
Oncogenic
miRNAs
(oncomiRs)
play
biological
role
in
initiation
and
progression
of
colorectal
cancer.
Inspired
by
cooperative
mechanisms
plant
nanovirus,
which
employ
multiple
circular
single‐stranded
DNA
(CssDNA)
genomes,
it
is
hypothesized
that
development
delivery
CssDNA
to
target
oncomiRs
would
achieve
benefits
CRC.
In
this
study,
multi‐omics
approach
utilized
identify
key
tumor
suppressor
genes
(TSGs)
their
related
implicated
CRC,
followed
CssDNA,
each
loaded
with
miRNA
binding
sites
targeting
one
oncomiR.
When
transfected
into
cells,
these
can
effectively
sequester
corresponding
restore
expression
TSGs,
leading
marked
reduction
CRC
both
vitro
vivo.
The
findings
highlight
potential
nanovirus‐inspired
modulating
miRNA‐mediated
regulatory
network
This
study
lays
groundwork
non‐coding
DNA‐based
therapies
broad
implications
treatment
potentially
other
malignancies.
Cell Proliferation,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 22, 2025
ABSTRACT
This
study
aimed
to
clarify
the
role
and
mechanism
of
tetrahedral
framework
nucleic
acids
(tFNAs)
in
regulating
M2
macrophages
reduce
intestinal
injury.
An
injury
model
was
established
by
intraperitoneal
injection
lipopolysaccharides
(LPS)
mice
explore
alleviating
effects
tFNAs
on
Inflammatory
factors
were
detected
quantitative
polymerase
chain
reaction
(qPCR)
enzyme‐linked
immunosorbent
assay
(ELISA).
The
barrier
permeability
assessed
using
western
blotting
immunohistochemistry.
Macrophages
gut
localised
quantified
immunofluorescence.
Western
used
investigate
inflammation
injured
intestines.
These
results
show
that
attenuated
sepsis‐induced
can
also
promote
reconstruction
permeability.
In
vivo,
accelerated
aggregation
at
an
early
stage
reduced
number
M1
intestine.
addition,
enhanced
clearance
ability
macrophages.
They
activated
signalling
transcription
activating
factor
1(STAT1)
cytokine
inhibitory
1/3
(SOCS1/3)
pathways
increasing
expression
phagocytic
receptor
Mertk.
findings
indicated
alleviate
macrophages,
providing
a
new
option
for
treating
Oral
squamous
cell
carcinoma
(OSCC)
represents
a
heterogeneous
group
of
malignancies
originating
from
the
mucosal
lining
oral
cavity.
Current
treatment
modalities
primarily
involve
surgery,
chemotherapy,
and
radiotherapy.
Despite
use
multimodal
therapy,
5
year
overall
survival
rate
for
OSCC
remains
around
50%,
underscoring
need
development
nontoxic
agents
with
potent
antitumor
activity.
Extracellular
vesicles
(EVs)
are
nanoscale,
membrane-bound
structures
that
can
selectively
deliver
small
molecules,
nucleic
acids,
proteins
to
target
cells,
making
them
promising
platform
drug
delivery
in
cancer
therapy.
Strategies
improve
uptake
EVs
enhance
therapeutic
molecules
cells
critical
advancing
precision
medicine.
Tetrahedral
DNA
nanostructures
(TDNs)
have
shown
significant
potential
facilitating
endocytosis
delivery,
as
well
improving
tissue
penetration.
In
this
study,
TDN@EVs
were
conducted
by
modifying
membrane
surface
M1-EVs
TDNs,
which
demonstrated
improved
biological
stability
efficiency
compared
unmodified
EVs.
vitro
vivo
experiments
showed
significantly
inhibited
proliferation
migration
while
promoting
apoptosis.
exhibited
superior
penetration
properties,
further
amplifying
their
effects.
Proteomic
analysis
identified
Hsc70
key
protein
responsible
activity
TDN@EVs.
The
efficient
into
tumor
led
degradation
GPX4,
inducing
ferroptosis,
mitochondrial
stress,
damage
cells.
These
findings
highlight
an
effective
safe
approach
conclusion,
present
strategy
targeted
treatment,
offering
enhanced
stability,
