Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Май 21, 2025
Protein sulfenylation (protein-SOH) is a central oxidation product of protein post-translational modification (PTM) that crucial for signal transduction and cell behavior. However, the natural properties protein-SOH, especially its low responsiveness dynamic reversibility, pose great challenge to development chemical probes visualize protein-SOH in vivo. Here, we report an activated aggregation-induced emission (AIE) probe specifically lighting-up The AIE-active reacts with by nucleophilic substitution exhibits intense fluorescence due restriction intramolecular motion. uniqueness this ensures only lighted up avoiding interference from small-molecule active substances nonspecific adsorption proteins. significant increase atherosclerotic mice detected AIE probe, level positively correlates atherosclerosis progression. Significantly, find specific binding can inhibit plaque development, making it promising therapeutic target. This study enables real-time imaging vivo, opening universal tool further elucidation PTM role transduction.
Язык: Английский