Balancing Permeability and Stability: A Study of Hybrid Membranes for Synthetic Cells Using Lipids and PBd-b-PEO Block Copolymers

Biomacromolecules, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

We have synthesized hybrid membranes composed of amphiphilic block copolymers, polybutadiene-poly(ethylene oxide) [PBd-b-PEO], with different lengths [PBd22-PEO14 and PBd11-PEO8] mixtures phospholipids (DOPC:DOPG:DOPE 50:25:25 mol %) to combine the properties both in terms stability fluidity membrane. The copolymers increase stability, whereas lipids support functionality membrane proteins. nature bilayers was studied by means Cryo-TEM, Langmuir-Blodgett technique, atomic force microscopy (AFM), electrical measurements, fluorescence-based stopped-flow assay determine permeability for water osmolytes. observe that structural, thermodynamic, PBd11-PEO8 are similar their purely lipid counterparts, advantage being more stable resisting a higher transmembrane potential. Hybrid longer polymer, PBd22-PEO14, display significant differences show less favorable than hybrid-PBd11-PEO8 membranes.

Язык: Английский

Nanopores with an Engineered Selective Entropic Gate Detect Proteins at Nanomolar Concentration in Complex Biological Sample

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Апрель 22, 2025

Язык: Английский