Low-Temperature Electrospinning-Fabricated Three-Dimensional Nanofiber Scaffolds for Skin Substitutes

Micromachines, Год журнала: 2025, Номер 16(5), С. 552 - 552

Опубликована: Апрель 30, 2025

Severe skin damage poses a significant clinical challenge, as limited availability of donors, postoperative defects, and scarring often impair function. Traditional two-dimensional (2D) nanofibers exhibit small pore sizes that hinder cellular infiltration, unable to simulate the three-dimensional (3D) structure skin. To address these issues, we developed 3D porous nanofiber scaffolds composed polycaprolactone–polylactic acid–mussel adhesive protein (PLGA-PCL-MAP) using low-temperature electrospinning combined with nano-spray technology. Meanwhile, this scaffold features high porosity, enhanced water absorption, improved air permeability. The incorporation mussel (MAP) further increased scaffold’s properties biocompatibility. In vitro experiments demonstrated significantly promoted adhesion, proliferation, migration epidermal keratinocytes (HaCaTs) human fibroblasts (HFBs), while providing ample space for inward growth. Successful co-culture HaCaT HFBs within revealed key functional outcomes: HaCaTs expressed keratinocyte differentiation markers CK10 CK14, actively secreted extracellular matrix components critical wound healing, including collagen I, III, fibronectin. This substitute composite epidermis dermis based on can be used an ideal replacement is expected applied in repair future.

Язык: Английский

Anisotropic composite scaffolds containing strontium-substituted hydroxyapatite nanofibers and collagen as bone substitutes

Polymer Testing, Год журнала: 2025, Номер unknown, С. 108875 - 108875

Опубликована: Май 1, 2025

Язык: Английский