Graphene Nanomaterial-Based Electrochemical Biosensors for Salivary Biomarker Detection: A Translational Approach to Oral Cancer Diagnostics

Nano TransMed, Год журнала: 2025, Номер unknown, С. 100073 - 100073

Опубликована: Янв. 1, 2025

Язык: Английский

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Multivalent Aptamer-Linked Tetrahedron DNA Assisted Catalytic Hairpin Assembly for Accurate Sers Assay of Cancer-Derived Exosomes in Clinical Blood

Опубликована: Янв. 1, 2025

Язык: Английский

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Emerging applications of biorecognition elements-based optical biosensors for food safety monitoring

Опубликована: Фев. 14, 2025

Язык: Английский

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Aptamers as Diagnostic and Therapeutic Agents for Aging and Age-Related Diseases

Biosensors, Год журнала: 2025, Номер 15(4), С. 232 - 232

Опубликована: Апрель 5, 2025

In the 21st century, demographic shift toward an aging population has posed a significant challenge, particularly with respect to age-related diseases, which constitute major threat human health. Accordingly, detection, prevention, and treatment of diseases have become critical issues, introduction novel molecular recognition elements, called aptamers, been considered. Aptamers, class oligonucleotides, can bind target molecules high specificity. addition, aptamers exhibit superior stability, biocompatibility, applicability, rendering them promising tools for diagnosis diseases. this paper, we present comprehensive overview systematic evolution ligands by exponential enrichment (SELEX), biomarkers associated aging, as well aptamer-based diagnostic therapeutic platforms. Finally, limitations predicting preventing conditions are discussed, along potential solutions based on advanced technologies theoretical approaches.

Язык: Английский

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Exploring the G-Quadruplex Formation of AS1411 Derivatives

Molecules, Год журнала: 2025, Номер 30(8), С. 1673 - 1673

Опубликована: Апрель 8, 2025

AS1411 is a G-quadruplex (G4) aptamer that binds tightly to nucleolin (NCL) on the cell surface and has shown strong anticancer effects. However, this highly polymorphic, presenting different types of G4s, which may hinder its preclinical application. Several modifications have been made decrease polymorphism aptamer. In work, we designed six derivatives by substituting guanine with thymine in central linker modifying number thymines either itself and/or at both ends sequence. The G4 formation, stability, NCL binding were evaluated several biophysical techniques computational studies. Overall, G4-forming sequences compared observed size exclusion chromatography (SEC) circular dichroism (CD) spectroscopy presence potassium salt. melting experiments reveal higher ability without sequence form G4, consistent G4H score predictions. Additionally, it possible conclude deletions T core increase G4. Moreover, bind high affinity (KD values 10-9 M range), particularly only linker. silico studies structural insights demonstrate interact NCL, establishing multiple interactions domains, thereby further supporting experimental findings. By using lung cancer line expression, evaluate internalization uptake derivatives, identifying derivative-lacking as ones highest cellular uptake.

Язык: Английский

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Multivalent aptamer-linked tetrahedron DNA assisted catalytic hairpin assembly for accurate SERS assay of cancer-derived exosomes in clinical blood

Biosensors and Bioelectronics, Год журнала: 2025, Номер 282, С. 117497 - 117497

Опубликована: Апрель 18, 2025

Язык: Английский

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A tag-free fluorescent aptasensor for ultrasensitive detection of sulfadimethoxine antibiotic mediated by rolling circle amplified DNA cascades

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2025, Номер 339, С. 126309 - 126309

Опубликована: Апрель 28, 2025

Язык: Английский

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Review of Recent Advances in Aptasensor for the Detection of Pathogenic Escherichia coli O157: H7

Journal of Food Safety, Год журнала: 2025, Номер 45(3)

Опубликована: Апрель 30, 2025

ABSTRACT Escherichia coli ( E. ) O157: H7 is a critical pathogen due to its association with severe conditions like hemorrhagic colitis and hemolytic uremic syndrome. The prevalence of this underscores the urgent need for effective food environmental safety measures. Among emerging innovations, aptamer‐based biosensors (aptasensors) offer increased sensitivity reduced detection times compared traditional methods identifying H7. Aptamers, short single‐stranded DNA (ssDNA) or RNA molecules, act as bio‐recognition elements, exhibiting high specificity affinity foodborne pathogens. This scoping review examines recent advancements in aptasensor technology over past 5 years, focusing on platforms targeting detection. Key elements such targets, sample types, transducers, sensing mechanisms, fabrication methods, limits were analyzed identify strengths limitations current platforms. findings indicate that most optical aptasensors, particularly fluorescence‐based ones, have been developed detecting water, food, milk samples. Currently developing aptasensors show promise, offering reliable alternatives improved capabilities. Nonetheless, further studies are still needed validate their facilitate broader applications. explores latest developments across diverse including electrochemical, optical, magnetic approaches, working principles, advantages, limitations. It highlights potential practical applications agricultural samples, emphasizing role advancing monitoring systems.

Язык: Английский

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Assembling ″Balloon-String″ Colorimetric Probes through Click Chemistry and Hybridization Chain Reaction for Dual-Signal Amplification in Aptamer-Based Lateral Flow Assays

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 10, 2025

As a simple and visually assessable point-of-care testing method, lateral flow assays (LFAs) are widely used for detecting procymidone in vegetable samples. Meanwhile, an increasing number of aptamer-based LFAs being developed rapid detection. However, the sensitivity conventional gold nanoparticles (AuNPs) is limited, which challenge to meet detection requirements. This study proposed dual amplification strategy click chemistry hybridization chain reaction (HCR) construct colorimetric probe enhance sensitivity. The nucleic acid nanostructure (HCR-ALK) constructed by HCR was as programming template guide chemical induce cross-linking AuNPs-N3 HCR-ALK form uniform stable ″ballon-string″ (HCR-AuNPs). achieved dual-signal amplification. Under optimal conditions, limit (LOD) this LFA 5.4 × 10-2 ng/mL. Compared with AuNPs LFAs, its LOD had been improved 13.69-fold, color display time reduced one-tenth. In specificity stability experiments, showed satisfactory performance. Moreover, it successfully applied samples, recovery rate maintained between 93.44%-104.00%. Thus, provides signal address issue insufficient LFAs. will offer new insights development

Язык: Английский

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Whispering-gallery mode-based optical biosensing: A review on development and applications in ultrasensitive biological detection and bioimaging

Analytica Chimica Acta, Год журнала: 2025, Номер 1364, С. 344249 - 344249

Опубликована: Май 28, 2025

Язык: Английский

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