Using magnetic resonance relaxometry to evaluate the safety and quality of induced pluripotent stem cell-derived spinal cord progenitor cells DOI Creative Commons
Jerome Tan, Jiahui Chen,

Daniel N. Roxby

и другие.

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 5, 2024

The emergence of induced pluripotent stem cells (iPSCs) offers a promising approach for replacing damaged neurons and glial cells, particularly in spinal cord injuries (SCI). Despite its merits, iPSC differentiation into progenitor (SCPCs) is variable, necessitating reliable assessment validation cell quality safety. Phenotyping often performed via label-based methods including immunofluorescent staining or flow cytometry analysis. These approaches are expensive, laborious, time-consuming, destructive, severely limits their use large scale therapy manufacturing settings. On the other hand, cellular biophysical properties have demonstrated strong correlation to state, functionality can be measured with ingenious label-free technologies rapid non-destructive manner. In this study, we report Magnetic Resonance Relaxometry (MRR), method that indicates iron levels based on readout (T2). Briefly, differentiated human iPSCs SCPCs compared key SCPC markers intracellular content (Fe3+) at different stages process. With MRR, found were distinctively allowing us accurately reflect varying residual undifferentiated (i.e., OCT4+ cells) any given population SCPCs. MRR was also able predict Day 10 OCT4 from 1 T2 values identified poorly lower T2, indicative neural (SOX1) (Nestin) marker expression levels. Lastly, provide predictive indications extent 28 motor (ISL-1/SMI-32) measurements has clearly indicated capabilities identify quantify phenotypes end-point safety parameters. Thus, our technology provides determine critical attributes iPSC-derived progenies ideally suited as control tool manufacturing.

Язык: Английский

Application of bioelectrical impedance detection techniques: cells and tissues DOI
Jianming Wen,

Pengjie Wu,

Jianping Li

и другие.

Biosensors and Bioelectronics, Год журнала: 2025, Номер 273, С. 117159 - 117159

Опубликована: Янв. 11, 2025

Язык: Английский

Процитировано

1
Using magnetic resonance relaxometry to evaluate the safety and quality of induced pluripotent stem cell-derived spinal cord progenitor cells DOI Creative Commons
Jerome Tan, Jiahui Chen,

Daniel N. Roxby

и другие.

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 5, 2024

The emergence of induced pluripotent stem cells (iPSCs) offers a promising approach for replacing damaged neurons and glial cells, particularly in spinal cord injuries (SCI). Despite its merits, iPSC differentiation into progenitor (SCPCs) is variable, necessitating reliable assessment validation cell quality safety. Phenotyping often performed via label-based methods including immunofluorescent staining or flow cytometry analysis. These approaches are expensive, laborious, time-consuming, destructive, severely limits their use large scale therapy manufacturing settings. On the other hand, cellular biophysical properties have demonstrated strong correlation to state, functionality can be measured with ingenious label-free technologies rapid non-destructive manner. In this study, we report Magnetic Resonance Relaxometry (MRR), method that indicates iron levels based on readout (T2). Briefly, differentiated human iPSCs SCPCs compared key SCPC markers intracellular content (Fe3+) at different stages process. With MRR, found were distinctively allowing us accurately reflect varying residual undifferentiated (i.e., OCT4+ cells) any given population SCPCs. MRR was also able predict Day 10 OCT4 from 1 T2 values identified poorly lower T2, indicative neural (SOX1) (Nestin) marker expression levels. Lastly, provide predictive indications extent 28 motor (ISL-1/SMI-32) measurements has clearly indicated capabilities identify quantify phenotypes end-point safety parameters. Thus, our technology provides determine critical attributes iPSC-derived progenies ideally suited as control tool manufacturing.

Язык: Английский

Процитировано

1