Sensors and Actuators B Chemical, Год журнала: 2024, Номер 427, С. 137180 - 137180
Опубликована: Дек. 27, 2024
Язык: Английский
Advanced Optical Materials, Год журнала: 2024, Номер 12(19)
Опубликована: Май 11, 2024
Abstract DNA‐stabilized silver nanoclusters (DNA‐AgNCs) are a class of emitters that have primarily been studied for their molecular‐like photophysical properties with ns‐lived fluorescence. However, µs‐lived luminescence has recently reported an increasing number DNA‐AgNCs, but little is still known about the origin long‐lived emission. To deepen understanding state, (DNA) 2 ‐[Ag 11 ] 7+ , emitter short‐ and dual emission, studied. By examining crystals it found fluorescence transitions orthogonal to each other, which implies components long axis AgNC, such as nucleobases, contribute significantly polarization character luminescent state. The effect hydration level on emission dried droplets also investigated, water drastically reduces from state causes redshift Finally, unusual excitation intensity‐dependent response noticed. This ascribed heterogeneous local environment amorphous solid resulting in different conformations optically activated delayed probabilities. presented results provide new key insights into nature DNA‐AgNCs.
Язык: Английский
Процитировано
6
Nanoscale, Год журнала: 2024, Номер 16(26), С. 12559 - 12566
Опубликована: Янв. 1, 2024
DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters formed by atoms and cations encapsulated in DNA oligomers. Here, we present an analytical approach to calculate the molar absorption coefficient (ε) of these systems, which consists combining UV-Vis spectroscopy, electrospray ionization-mass spectrometry (ESI-MS), inductively coupled plasma-optical emission (ICP-OES). ESI-MS enables determination number silvers bound strands, whereas ICP-OES allows measurement total amount solution. The data is used concentration DNA-AgNCs together with absorbance, for calculation ε. We compare obtained ε experimental values previously determined through fluorescence correlation spectroscopy (FCS) theoretical estimates based on itself. Finally, radiative decay rates (kf) evaluated compared those typically found organic fluorophores, highlighting molecular-like nature DNA-AgNC emission.
Язык: Английский
Процитировано
5
ACS Nano, Год журнала: 2024, Номер unknown
Опубликована: Сен. 17, 2024
DNA-stabilized silver nanoclusters (Ag
Язык: Английский
Процитировано
5
Accounts of Chemical Research, Год журнала: 2024, Номер 57(15), С. 2117 - 2129
Опубликована: Июль 12, 2024
ConspectusDNA-stabilized silver nanoclusters (Ag
Язык: Английский
Процитировано
4
Advanced Optical Materials, Год журнала: 2025, Номер unknown
Опубликована: Янв. 16, 2025
Abstract DNA‐stabilized silver nanoclusters (DNA‐Ag N ) are atomically precise and sequence‐tuned nanomaterials with potential applications for deep tissue biomedical imaging. Here, a dual‐emissive DNA‐Ag is presented fluorescence in the first near‐infrared (NIR‐I) spectral window microsecond‐lived photoluminescence second (NIR‐II) window. High‐resolution electrospray ionization mass spectrometry showed that emitter has molecular formula (DNA) 2 [Ag 17 ] 11+ . The crystallization of was unsuccessful, which prevented use X‐ray diffraction to determine its structure. However, sequence variations templating DNA oligomer provided insights into nucleobases critical stabilizing Ag Moreover, addition an adenosine or thymidine at 5′‐end strand maintained composition photophysical properties , suggesting site conjugation biomolecules enable targeted labeling future bioimaging applications.
Язык: Английский
Процитировано
0
Small Structures, Год журнала: 2025, Номер unknown
Опубликована: Март 17, 2025
Fluorescence imaging is a key tool in biological and medical sciences. Despite the potential for increased depth near‐infrared range, limited availability of bright emitters hinders its widespread implementation. In this work, DNA‐stabilized silver nanocluster (DNA–AgNC) with emission at 960 nm solution presented, which redshifts further to 1055 solid crystalline states. The atomic structure, composition charge DNA–AgNC are determined by combining single‐crystal X‐ray diffraction electrospray ionization–mass spectrometry. This unique atomically precise consists 28 atoms, neutral (Ag 16+ ), arranged rodlike shape, measures just over 2 length. Interestingly, differences observed number chlorido ligands between states, highlighting important but not yet fully understood role chlorides fine‐tuning optical properties class emitters. structure nanorod, along characterized photophysical properties, represents cornerstone understanding intricate interactions DNA bases, as well paving way rational design next‐generation probes.
Язык: Английский
Процитировано
0
The Journal of Physical Chemistry B, Год журнала: 2023, Номер 127(50), С. 10851 - 10860
Опубликована: Дек. 6, 2023
A DNA strand can encapsulate a silver molecule to create nanoscale, aqueous stable chromophore. protected cluster that strongly fluoresces also be weakly photolabile, and we describe the laser-driven photochemistry of green fluorophore C4AC4TC3GT4/Ag106+. The embedded is selectively photoexcited at 490 nm then bleached, how efficiency, products, route this photochemical reaction are controlled by cage. With irradiation 496.5 nm, absorption progressively drops give photodestruction quantum yield 1.5 (±0.2) × 10-4, ∼103× less efficient than fluorescence. new λabs = 335 chromophore develops because precursor with 4 Ag0 converted into group clusters 2 - Ag64+, Ag75+, Ag86+, Ag97+. 4-7 Ag+ in series chemically distinct from they etched iodide. This halide precipitates favor only smallest Ag64+ cluster, but larger re-develop when precipitated ions replenished. DNA-bound Ag106+ decomposes it electronically excited reacts oxygen. two-step process may state-specific O2 quenches red luminescence Ag106+. However, rate constant 2.3 106 M-1 s-1 relatively small, which suggests surrounding matrix hinders diffusion. On basis analogous photoproducts methylene blue, propose reactive oxygen species produced oxidizes leave behind loose Ag+-DNA skeleton. These findings underscore ability scaffolds not tune spectra guide reactions their molecular adducts.
Язык: Английский
Процитировано
7
Nano Letters, Год журнала: 2024, Номер 24(26), С. 7987 - 7991
Опубликована: Июнь 21, 2024
DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of fluorophores with interesting photophysical properties dominated by the choice DNA sequence. Screening methods ultraviolet excitation and steady state well plate readers have previously been used for deepening understanding between sequence emission color resulting DNA-AgNCs. Here, we present new method screening DNA-AgNCs using pulsed white light (λ
Язык: Английский
Процитировано
2
The Journal of Physical Chemistry Letters, Год журнала: 2024, Номер 15(42), С. 10710 - 10717
Опубликована: Окт. 17, 2024
The luminescent quantum yield of silver-cluster emitters stabilized by short oligonucleotides (AgN-DNA) may be efficiently tuned replacing nucleobases in their stabilization DNA matrices with analogues. In the present study, we proposed a valuable and straightforward theoretical methodology for assessing photophysical behaviors emerging AgN-DNA after excitation. Using green Ag10-DNA near-IR Ag16-DNA demonstrate how point guanine/inosine replacement could affect rate constants radiative/nonradiative processes. main deactivation channel fluorescence is intersystem crossing, which line experimental data, whereas calculations overestimate crossing possibly due to pure solvent contributions.
Язык: Английский
Процитировано
1
Nanoscale, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Silver cluster–DNA conjugates catalyze alkyne–azide cycloadditions. The catalytic activity of the cluster is regulated by DNA host.
Язык: Английский
Процитировано
0