Soft X-ray 3D Imaging: A Powerful Tool for Visualizing Virus Infections with Increased Resolution and Field of View DOI Open Access
Jian-Hua Chen, Bieke Vanslembrouck, Axel Ekman

и другие.

Microscopy and Microanalysis, Год журнала: 2023, Номер 29(Supplement_1), С. 1155 - 1156

Опубликована: Июль 22, 2023

Journal Article Soft X-ray 3D Imaging: A Powerful Tool for Visualizing Virus Infections with Increased Resolution and Field of View Get access Jian-Hua Chen, Chen Molecular Biophysics Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States AmericaDepartment Anatomy, University California San Francisco, America Search other works by this author on: Oxford Academic Google Scholar Bieke Vanslembrouck, Vanslembrouck Axel Ekman, Ekman Department Valentina Loconte, Loconte Venera Weinhardt, Weinhardt Infectious Diseases, Virology Heidelberg University, Heidelberg, Germany Mark LeGros, LeGros Carolyn Larabell Microscopy Microanalysis, Volume 29, Issue Supplement_1, 1 August 2023, Pages 1155–1156, https://doi.org/10.1093/micmic/ozad067.590 Published: 22 July 2023

Язык: Английский

Soft X-ray tomography reveals variations in B. subtilis biofilm structure upon tasA deletion DOI Creative Commons
Anthoula Chatzimpinou, Anne Diehl,

A Tobias Harhoff

и другие.

npj Biofilms and Microbiomes, Год журнала: 2025, Номер 11(1)

Опубликована: Фев. 2, 2025

Bacterial biofilms are complex cell communities within a self-produced extracellular matrix, crucial in various fields but challenging to analyze 3D. We developed "biofilm-in-capillary" growth method compatible with full-rotation soft X-ray tomography, enabling high-resolution 3D imaging of bacterial cells and their matrix during biofilm formation. This approach offers 50 nm isotropic spatial resolution, rapid imaging, quantitative native analysis structure. Using Bacillus subtilis biofilms, we detected coherent alignment chaining wild-type towards the oxygen-rich capillary tip. In contrast, ΔtasA genetic knock-out showed loss cellular orientation changes matrix. Adding TasA protein strain restored density led assembly compaction, without observed biofilms. scalable transferable opens new avenues for examining structure function across species, including mixed response environmental factors.

Язык: Английский

Процитировано

0

Limited-angle artifacts removal and jitter correction in soft x-ray tomography via physical model-driven deep learning DOI Open Access
Xiayu Tao, Zheng Dang,

Yutong Zheng

и другие.

Applied Physics Letters, Год журнала: 2023, Номер 123(19)

Опубликована: Ноя. 6, 2023

Soft x-ray nanoscale tomography provides high-resolution three-dimensional visualization of the imaged objects and promotes development multiple research fields. However, current challenges lie in presence limited-angle artifacts projection jitter, which degrade imaging resolution quality. To address these issues, we propose a physical model-driven deep learning including forward backward CT models. Combing with iterative algorithm, proposed method simultaneously suppresses jitter artifacts. Furthermore, model generates plenty data to overcome requirement abundant experimental datasets. Both simulation experiment demonstrate feasibility validity reconstruction algorithm.

Язык: Английский

Процитировано

4

Subcellular Feature-Based Classification of α and β Cells Using Soft X-ray Tomography DOI Creative Commons
Aneesh Deshmukh, Kevin Chang, Janielle Cuala

и другие.

Cells, Год журнала: 2024, Номер 13(10), С. 869 - 869

Опубликована: Май 18, 2024

The dysfunction of α and β cells in pancreatic islets can lead to diabetes. Many questions remain on the subcellular organization islet during progression disease. Existing three-dimensional cellular mapping approaches face challenges such as time-intensive sample sectioning subjective identification. To address these challenges, we have developed a feature-based classification approach, which allows us identify quantify their structural characteristics using soft X-ray tomography (SXT). We observed significant differences whole-cell morphological organelle statistics between two cell types. Additionally, characterize subtle biophysical individual insulin glucagon vesicles by analyzing vesicle size molecular density distributions, were not previously possible other methods. These sub-vesicular parameters enable predict types systematically supervised machine learning. also visualize distinct subtypes Uniform Manifold Approximation Projection (UMAP) embeddings, provides with an innovative approach explore heterogeneity cells. This methodology presents for tracking biologically meaningful that be applied any system.

Язык: Английский

Процитировано

1

Optimization of Soft X-Ray Fresnel Zone Plate Fabrication Through Joint Electron Beam Lithography and Cryo-Etching Techniques DOI Creative Commons
Maha Labani, Vito Clericò, E. Díez

и другие.

Nanomaterials, Год журнала: 2024, Номер 14(23), С. 1898 - 1898

Опубликована: Ноя. 26, 2024

The ability to manufacture complex 3D structures with nanometer-scale resolution, such as Fresnel Zone Plates (FZPs), is crucial achieve state-of-the-art control in X-ray sources for use a diverse range of cutting-edge applications. This study demonstrates novel approach combining Electron Beam Lithography (EBL) and cryoetching produce silicon-based FZP prototypes test bench assess the strong points limitations this fabrication method. Through method, we obtained FZPs 100 zones, diameter 20 µm, an outermost zone width 50 nm, resulting high aspect ratio that suitable across photon energies. process incorporates chromium mask EBL stage, enhancing microstructure precision mitigating pattern collapse challenges. minimized issues under- over-etching, producing well-defined patterns resolution low roughness. refined thus holds promise achieving improved optical efficiency FZPs, making it viable high-performance, devices.

Язык: Английский

Процитировано

1

Absorption‐coefficient calculation of short‐wavelength photoresist materials: From EUV to BEUV and water window X‐ray DOI Creative Commons

Yifeng Peng,

Pengzhong Chen, Hao Chen

и другие.

Smart Molecules, Год журнала: 2024, Номер unknown

Опубликована: Дек. 16, 2024

Abstract In photolithography, shortening the exposure wavelength from ultraviolet to extreme (EUV, 13.5 nm) and soft X‐ray region in terms of beyond EUV (BEUV, 6.X water window (WWX, 2.2–4.4 is expected further miniaturize technology node down sub‐5 nm level. However, absorption ability molecules these ranges, especially WWX region, unknown, which should be very important for utilization energy. Herein, molar cross sections different elements at 2.4 were firstly calculated compared with wavelengths 6.7 nm. Based on ranges density estimation results density‐functional theory calculation, linear coefficients typical resist materials, including metal‐oxy clusters, organic small molecules, polymers, photoacid generators (PAGs), are evaluated. The analysis suggests that Zn cluster has higher BEUV, whereas Sn WWX. Doping PAGs high atoms improves chemically amplified photoresist (CAR) polymer performance. WWX, it necessary introduce an layer containing such as Zr, Sn, Hf increase absorption.

Язык: Английский

Процитировано

1

Dehydration as alternative sample preparation for soft X‐ray tomography DOI Creative Commons
Anthoula Chatzimpinou, Charlotta Funaya,

D. C. Rogers

и другие.

Journal of Microscopy, Год журнала: 2023, Номер 291(3), С. 248 - 255

Опубликована: Июль 12, 2023

Abstract Soft X‐ray tomography (SXT) is an imaging technique to visualise whole cells without fixation, staining, and sectioning. For SXT imaging, are cryopreserved imaged at cryogenic conditions. Such ‘near‐to‐native’ state in high demand initiated the development of laboratory table‐top microscope. As many laboratories do not have access equipment, we asked ourselves whether feasible on dry specimens. This paper shows how dehydration can be used as alternative sample preparation obtain ultrastructure information. We compare different processes mouse embryonic fibroblasts terms ultrastructural preservation shrinkage. Based this analysis, chose critical point (CPD) dried for imaging. In comparison air‐dried cells, CPD dehydrated show structural integrity although with about 3–7 times higher absorption cellular organelles. difference values between organelles preserved, 3D anatomy CPD‐dried segmented analysed, demonstrating applicability LAY DESCRIPTION : that allows see internal structures need special treatments like fixation or staining. Typically, involves freezing very low temperatures. However, since labs lack necessary explored could done samples instead. compared methods found drying was most promising showed integrity, they absorbed more X‐rays than hydrated a viable

Язык: Английский

Процитировано

1

Soft X-ray 3D Imaging: A Powerful Tool for Visualizing Virus Infections with Increased Resolution and Field of View DOI Open Access
Jian-Hua Chen, Bieke Vanslembrouck, Axel Ekman

и другие.

Microscopy and Microanalysis, Год журнала: 2023, Номер 29(Supplement_1), С. 1155 - 1156

Опубликована: Июль 22, 2023

Journal Article Soft X-ray 3D Imaging: A Powerful Tool for Visualizing Virus Infections with Increased Resolution and Field of View Get access Jian-Hua Chen, Chen Molecular Biophysics Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States AmericaDepartment Anatomy, University California San Francisco, America Search other works by this author on: Oxford Academic Google Scholar Bieke Vanslembrouck, Vanslembrouck Axel Ekman, Ekman Department Valentina Loconte, Loconte Venera Weinhardt, Weinhardt Infectious Diseases, Virology Heidelberg University, Heidelberg, Germany Mark LeGros, LeGros Carolyn Larabell Microscopy Microanalysis, Volume 29, Issue Supplement_1, 1 August 2023, Pages 1155–1156, https://doi.org/10.1093/micmic/ozad067.590 Published: 22 July 2023

Язык: Английский

Процитировано

0