Engineered Extracellular Vesicles Derived from Juvenile Mice Enhance Mitochondrial Function in the Aging Bone Microenvironment and Achieve Rejuvenation
ACS Nano,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 4, 2025
Aging-related
bone
degeneration
and
impaired
healing
capacity
remain
significant
challenges
in
regenerative
medicine,
necessitating
innovative,
efficient,
targeted
strategies
to
restore
health.
Here,
we
engineered
extracellular
vesicles
(EVs)
derived
from
the
serum
of
pretreated
juvenile
mice,
with
goals
reversing
aging,
enhancing
osteogenic
potential,
increasing
bioavailability
rejuvenate
aging
environment.
First,
established
models
representing
different
phases
identify
EV
type
highest
potential
for
improving
microenvironment
older
individuals.
Second,
employed
DSS6
targeting
enhance
biological
effects
selected
EVs
vivo.
The
effectively
repair
sites
promoted
fracture
more
than
unmodified
mice.
RNA
sequencing
revealed
that
translocase
outer
mitochondrial
membrane
7
(Tomm7)
is
crucial
underlying
mechanism.
Silencing
Tomm7
significantly
diminished
positive
regulatory
EVs.
Specifically,
may
function
cells
by
activating
Tomm7-mediated
Pink1/Parkin
mitophagy
pathway,
promoting
stemness
recovery
marrow
stromal
(BMSCs)
adverse
conditions
microenvironment.
Overall,
developed
mice
offer
an
alternative
approach
treating
bones.
identified
mechanisms
provide
a
valuable
reference
precision
treatment
bones
future.
Язык: Английский
From Bench to Bedside: The Role of Extracellular Vesicles in Cartilage Injury Treatment
Biomaterials Research,
Год журнала:
2024,
Номер
28
Опубликована: Янв. 1, 2024
Cartilage
repair
is
the
key
to
treatment
of
joint-related
injury.
However,
because
cartilage
lacks
vessels
and
nerves,
its
self-repair
ability
extremely
low.
Extracellular
vesicles
(EVs)
are
bilayer
nanovesicles
with
membranes
mainly
composed
ceramides,
cholesterol,
phosphoglycerides,
long-chain
free
fatty
acids,
containing
DNA,
RNA,
proteins
(such
as
integrins
enzymes).
For
mediating
intercellular
communication
regulating
mechanisms,
EVs
have
been
shown
by
multiple
studies
be
effective
options
for
repair.
This
review
summarizes
recent
findings
different
sources
(mammals,
plants,
bacteria)
uses
in
repair,
mechanisms
captured
injured
chondrocytes,
quantification
storage
EVs,
which
may
provide
scientific
guidance
promoting
development
field
injury
treatment.
Язык: Английский