PSC1, a basic/helix–loop–helix transcription factor controlling the purplish‐red testa trait in peanut

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 24, 2025

ABSTRACT Seed color is a key agronomic trait in crops such as peanut, where it vital indicator of both nutritional and commercial value. In recent years, peanuts with darker seed coats have gained market attention due to their high anthocyanin content. Here, we used bulk segregant analysis identify the gene associated purplish‐red coat identified novel encoding basic/helix–loop–helix transcription factor, P URPLE RED S EED C OAT1 ( PSC1 ), which regulates accumulation anthocyanins coat. Specifically, found that 35‐bp insertion promoter increased abundance mRNA. Transcriptomic metabolomic analyses indicated was result decreased expression anthocyanidin reductase (ANR), leading delphinidin, cyanidin, pelargonidin derivatives. Further revealed interacts AhMYB7 form complex specifically binds ANR suppress its expression, resulting accumulation. Moreover, overexpression content Arabidopsis thaliana peanut callus. Our study reveals new controls by regulating metabolism provides valuable genetic resource for breeding

Язык: Английский

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Pangenome analysis reveals structural variation associated with seed size and weight traits in peanut

Nature Genetics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

Язык: Английский

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Fine-Mapping of a Wild Genomic Region Involved in Pod and Seed Size Reduction on Chromosome A07 in Peanut (Arachis hypogaea L.)

Genes, Год журнала: 2020, Номер 11(12), С. 1402 - 1402

Опубликована: Ноя. 25, 2020

Fruit and seed size are important yield component traits that have been selected during crop domestication. In previous studies, Advanced Backcross Quantitative Trait Loci (AB-QTL) Chromosome Segment Substitution Line (CSSL) populations were developed in peanut by crossing the cultivated variety Fleur11 a synthetic wild allotetraploid (Arachis ipaensis × Arachis duranensis)4x. AB-QTL population, major QTL for pod was detected ~5 Mb interval proximal region of chromosome A07. CSSL line 12CS_091, which carries produces smaller pods seeds than Fleur11, identified. this study, we used two-step strategy to fine-map on We new SSR SNP markers, as well near-isogenic lines (NILs) target region. first located ~1 between two thanks genotyping large F2 population 2172 individuals single marker analysis approach. then nine markers evenly distributed refined genotype 490 F3 plants derived from 88 F2, 10 NILs. The phenotyping NILs marker/trait association allowed us narrowing down 168.37 kb segment, SNPs Aradu_A07_1148327 Aradu_A07_1316694. This contains 22 predicted genes. Among these genes, Aradu.DN3DB Aradu.RLZ61, encode transcriptional regulator STERILE APETALA-like (SAP) an F-box SNEEZY (SNE), respectively, particular interest. function genes regulating variation fruit is discussed. study will contribute better knowledge targeted

Язык: Английский

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Genome-wide investigation of defensin genes in peanut (Arachis hypogaea L.) reveals AhDef2.2 conferring resistance to bacterial wilt

The Crop Journal, Год журнала: 2021, Номер 10(3), С. 809 - 819

Опубликована: Ноя. 29, 2021

Peanut (Arachis hypogea L.) bacterial wilt (BW) is a devastating disease caused by Ralstonia solanacearum that results in severe yield and quality losses. Plant defensins are short cysteine-rich peptides with antimicrobial activity. The role of defensin genes (AhDef) peanut unclear. A genome-wide investigation AhDef was undertaken, 12 identified were classified into two groups containing the gamma-thionin domain formed four disulfide pairs: Cys1-Cys8, Cys2-Cys5, Cys3-Cys6, Cys4-Cys7. In silico analysis revealed showed highly conserved architectural features contained cis-elements associated phytohormone signaling defense responses. resistant cultivar, H108 (R) susceptible accession, H107 (S) tested R. inoculation. fewer symptoms than owing to inhibition reproduction spread vascular bundles roots stems. transcriptomic expression profile, genes, particularly AhDef1.6 AhDef2.2, up-regulated compared under infection treatment. Subcellular localization AhDef2.2 proteins both expressed specifically on plasma membrane. Overexpression protein fusion AhDef2.2-YFP Nicotiana benthamiana leaves increased resistance solanacearum, suggesting its response BW infection. may be valuable for breeding.

Язык: Английский

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Coordinated Lipid Mobilization during Seed Development and Germination in Peanut (Arachis hypogaea L.)

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 72(6), С. 3218 - 3230

Опубликована: Дек. 29, 2023

Peanut (Arachis hypogaea L.) is one of the most important oil crops in world due to its lipid-rich seeds. Lipid accumulation and degradation play crucial roles peanut seed maturation seedling establishment, respectively. Here, we utilized lipidomics transcriptomics comprehensively identify lipids associated functional genes that are development germination processes a large-seed variety. A total 332 were identified; triacylglycerols (TAGs) diacylglycerols abundant during maturation, constituting 70.43 16.11%, respectively, lipids. Significant alterations lipid profiles observed throughout germination. Notably, TAG (18:1/18:1/18:2) (18:1/18:2/18:2) peaked at 23386.63 23392.43 nmol/g, final stage development. Levels hydroxylated TAGs (HO-TAGs) increased significantly initial Accumulation patterns revealed an inverse relationship between free fatty acids TAGs. was determined be regulated by diacylglycerol acyltransferase, triacylglycerol lipase, transcription factors, predominantly yielding oleic acid, linoleic linolenic acid. Collectively, results this study provide valuable insights into dynamics peanuts, gene resources, guiding future research economically crop.

Язык: Английский

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WRKY transcription factors modulate flowering time in four Arachis species: a bioinformatics analysis

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Июнь 28, 2024

Abstract Background WRKY proteins are important transcription factors (TFs) in plants, involved growth and development responses to environmental changes. Although TFs have been studied at the genome level Arachis genus, including oil crop turfgrass, their regulatory networks controlling flowering time remain unclear. The aim of this study was predict molecular mechanisms regulation genus using bioinformatics approaches. Results flowering-time genes were retrieved from gene database. between downstream predicted tools. results showed that aging, autonomous, circadian clock, hormone, photoperiod, sugar, temperature, vernalization pathways modulate duranensis , ipaensis monticola hypogaea cv. Tifrunner. TF binding sites homologous exhibited asymmetric evolutionary pattern, indicating interact with other four species. Protein interaction network analysis interacted FRUITFULL APETALA2 implicated regulating had low expression levels, whereas varying patterns 22 tissues A. These indicate exhibit antagonistic or synergistic interactions associated proteins. Conclusions This reveals complex through which species

Язык: Английский

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Deciphering evolutionary dynamics of WRKY genes in Arachis species

BMC Genomics, Год журнала: 2023, Номер 24(1)

Опубликована: Янв. 27, 2023

Cultivated peanut (Arachis hypogaea), a progeny of the cross between A. duranensis and ipaensis, is an important oil protein crop from South America. To date, at least six Arachis genomes have been sequenced. WRKY transcription factors (TFs) play crucial roles in plant growth, development, response to abiotic biotic stresses. TFs identified duranensis, hypogaea cv. Tifrunner; however, variations their number evolutionary patterns across various spp. remain unclear.WRKY were compared different species, including monticola, cultivars (cv.) Fuhuasheng, Shitouqi, Tifrunner. The results showed that underwent dynamic equilibrium diploid tetraploid characterized by loss old retention new ones. Notably, cultivated peanuts inherited more conserved orthologs wild than donors. Analysis W-box elements protein-protein interactions revealed domestication processes affected evolution varieties. Fuhuasheng Shitouqi exhibited similar process, while those Tifrunner same species process based on interaction analysis.This study provides insights into

Язык: Английский

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A Single‐Nucleus Resolution Atlas of Transcriptome and Chromatin Accessibility for Peanut (Arachis Hypogaea L.) Leaves

Advanced Biology, Год журнала: 2023, Номер 8(1)

Опубликована: Окт. 12, 2023

Abstract The peanut is an important worldwide cash‐crop for edible oil and protein. However, the kinetic mechanisms that determine gene expression chromatin accessibility during leaf development in represented allotetraploid leguminous crops are poorly understood at single‐cell resolution. Here, a single‐nucleus atlas of leaves developed by simultaneously profiling transcriptome same individual‐cell using fluorescence‐activated sorted single‐nuclei. In total, 5930 cells with 50 890 expressed genes classified into 18 cell‐clusters, 5315 fragments enriched 26 083 target accessible landscape. developmental trajectory analysis reveals involvement ethylene‐AP2 module cell differentiation, cell‐cycle demonstrated genome replication featured distinct cell‐types circadian rhythms transcription factors (TFs). Furthermore, dual‐omics illustrates fatty acid pathway modulates epidermal‐guard differentiation providescritical TFs interaction networks understanding mesophyll development, cytokinin ( LHY / LOG ) regulates vascular growth. Additionally, AT‐hook protein AhAHL11 identified promotes area expansion modulating auxin content increase. summary, simultaneous landscapes snRNA/ATAC‐seq provides novel biological insights dynamic processes cellular level.

Язык: Английский

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Investigation of NLR Genes Reveals Divergent Evolution on NLRome in Diploid and Polyploid Species in Genus Trifolium

Genes, Год журнала: 2023, Номер 14(4), С. 867 - 867

Опубликована: Апрель 4, 2023

Crop wild relatives contain a greater variety of phenotypic and genotypic diversity compared to their domesticated counterparts. Trifolium crop species have limited genetic cope with biotic abiotic stresses due artificial selection for consumer preferences. Here, we investigated the distribution evolution nucleotide-binding site leucine-rich repeat receptor (NLR) genes in genus objective identify reference NLR genes. We identified 412, 350, 306, 389 241 were from Trifolium. subterraneum, T. pratense, occidentale, subgenome-A repens subgenome-B repens, respectively. Phylogenetic clustering analysis reveals seven sub-groups Specific subgroups such as G4-CNL, CCG10-CNL TIR-CNL show distinct duplication patterns specific species, which suggests subgroup duplications that are hallmarks divergent evolution. Furthermore, our results strongly suggest overall expansion repertoire subterraneum is gene events birth families after speciation. Moreover, NLRome allopolyploid has evolved asymmetrically, subgenome -A showing expansion, while underwent contraction. These findings provide crucial background data comprehending Fabaceae family offer more comprehensive disease resistance

Язык: Английский

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Chromosome‐level genome of Poropuntius huangchuchieni provides a diploid progenitor‐like reference genome for the allotetraploid Cyprinus carpio

Molecular Ecology Resources, Год журнала: 2021, Номер 21(5), С. 1658 - 1669

Опубликована: Фев. 24, 2021

Abstract The diploid Poropuntius huangchuchieni in the cyprinid family, which is widely distributed Mekong and Red River basins, one of most closely related progenitor‐like species allotetraploid common carp, was generated by merging two genomes during evolution. Therefore, P. genome essential for polyploid evolution studies Cyprinidae. Here, we report a high‐quality chromosome‐level assembly integrating Oxford Nanopore Hi‐C technologies. assembled size 1,021.38 Mb, 895.66 Mb anchored onto 25 chromosomes with N50 32.93 Mb. contained 486.28 repetitive elements 24,099 protein‐coding genes. Approximately 95.9% complete BUSCOs were detected, suggesting high completeness genome. Evolutionary analysis revealed that diverged from Cyprinus carpio at approximately 12 Mya. Genome comparison between B subgenome C. provided insights into chromosomal rearrangements speciation. With gene set, 17,474 orthologous genes identified , providing broad view component genome, critical future genetic analyses. genomic data set created provides reference adaptation carps.

Язык: Английский

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