Multimaterial 3D and 4D Bioprinting of Heterogenous Constructs for Tissue Engineering

Advanced Materials, Год журнала: 2023, Номер 36(34)

Опубликована: Сен. 22, 2023

Additive manufacturing (AM), which is based on the principle of layer-by-layer shaping and stacking discrete materials, has shown significant benefits in fabrication complicated implants for tissue engineering (TE). However, many native tissues exhibit anisotropic heterogenous constructs with diverse components functions. Consequently, replication biomimetic using conventional AM processes a single material challenging. Multimaterial 3D 4D bioprinting (with time as fourth dimension) emerged promising solution constructing multifunctional that can mimic host microenvironment better than single-material alternatives. Notably, 4D-printed multimaterial architectures provide time-dependent programmable dynamic promote cell activity regeneration response to external stimuli. This paper first presents typical design strategies TE applications. Subsequently, latest are discussed, along their advantages challenges. In particular, potential smart highlighted. Furthermore, this review provides insights into how facilitate realization next-generation

Язык: Английский

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Additive manufacturing of highly entangled polymer networks

Science, Год журнала: 2024, Номер 385(6708), С. 566 - 572

Опубликована: Авг. 1, 2024

Incorporation of polymer chain entanglements within a single network can synergistically improve stiffness and toughness, yet attaining such dense through vat photopolymerization additive manufacturing [e.g., digital light processing (DLP)] remains elusive. We report facile strategy that combines dark polymerization to allow constituent chains densely entangle as they form printed structures. This generalizable approach reaches high monomer conversion at room temperature without the need for additional stimuli, or heat after printing, enables highly entangled hydrogels elastomers exhibit fourfold- sevenfold-higher extension energies in comparison traditional DLP. used this method print high-resolution multimaterial structures with features spatially programmed adhesion wet tissues.

Язык: Английский

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Microgels for Cell Delivery in Tissue Engineering and Regenerative Medicine

Nano-Micro Letters, Год журнала: 2024, Номер 16(1)

Опубликована: Июнь 17, 2024

Abstract Microgels prepared from natural or synthetic hydrogel materials have aroused extensive attention as multifunctional cells drug carriers, that are promising for tissue engineering and regenerative medicine. can also be aggregated into microporous scaffolds, promoting cell infiltration proliferation repair. This review gives an overview of recent developments in the fabrication techniques applications microgels. A series conventional novel strategies including emulsification, microfluidic, lithography, electrospray, centrifugation, gas-shearing, three-dimensional bioprinting, etc. discussed depth. The characteristics microgels microgel-based scaffolds culture delivery elaborated with emphasis on advantages these carriers therapy. Additionally, we expound ongoing foreseeable current limitations their aggregate field biomedical engineering. Through stimulating innovative ideas, present paves new avenues expanding application techniques.

Язык: Английский

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Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing

Chemical Reviews, Год журнала: 2024, Номер 124(14), С. 8787 - 8822

Опубликована: Июль 5, 2024

Harnessing light for cross-linking of photoresponsive materials has revolutionized the field 3D printing. A wide variety techniques leveraging broad-spectrum shaping have been introduced as a way to achieve fast and high-resolution printing, with applications ranging from simple prototypes biomimetic engineered tissues regenerative medicine. Conventional light-based printing use material in layer-by-layer fashion produce complex parts. Only recently, new emerged which deploy multidirection, tomographic, light-sheet or filamented image projections deep into volume resin-filled vat photoinitiation cross-linking. These Deep Vat (DVP) approaches alleviate need layer-wise enable unprecedented fabrication speeds (within few seconds) high resolution (>10 μm). Here, we elucidate physics chemistry these processes, their commonalities differences, well emerging biomedical non-biomedical fields. Importantly, highlight limitations, future scope research that will improve scalability applicability DVP engineering medicine applications.

Язык: Английский

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Instantly adhesive and ultra-elastic patches for dynamic organ and wound repair

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июнь 3, 2024

Bioadhesive materials and patches are promising alternatives to surgical sutures staples. However, many existing bioadhesives do not meet the functional requirements of current procedures interventions. Here, we present a translational patch material that exhibits instant adhesion tissues (2.5-fold stronger than Tisseel, an FDA-approved fibrin glue), ultra-stretchability (stretching >300% its original length without losing elasticity), compatibility with rapid photo-projection (<2 min fabrication time/patch), ability deliver therapeutics. Using our established for in silico design optimization anisotropic-auxetic patches, created next-generation attachment while conforming broad range organ mechanics ex vivo vivo. Patches coated extracellular vesicles derived from mesenchymal stem cells demonstrate robust wound healing capability inducing foreign body response need removal can cause pain bleeding. We further single material-based, void-filling auxetic designed treatment lung puncture wounds.

Язык: Английский

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An Implantable Biohybrid Neural Interface Toward Synaptic Deep Brain Stimulation

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 9, 2025

Abstract In patients with sensory nerve loss, such as those experiencing optic damage that leads to vision the thalamus no longer receives corresponding input. To restore functional input, it is necessary bypass damaged circuits, which can be achieved by directly stimulating appropriate thalamic nuclei. However, available deep brain stimulation electrodes do not provide resolution required for effective restoration. Therefore, this work develops an implantable biohybrid neural interface aimed at innervating and synaptically targets. The combines a stretchable array aligned microfluidic axon guidance system seeded spheroids facilitate development of 3 mm long nerve‐like structure. A bioresorbable hydrogel conduit used bridge between tissue implant. Stimulation within structure in vitro use high‐density CMOS microelectrode arrays show faithful activity conduction across device. Although vivo innervation synapse formation has yet been study, implantation onto mouse cortex shows grow axons remain functionally active more than 22 days post‐implantation.

Язык: Английский

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From pixels to voxels: A mechanistic perspective on volumetric 3D-printing

Progress in Polymer Science, Год журнала: 2023, Номер 147, С. 101755 - 101755

Опубликована: Окт. 20, 2023

Язык: Английский

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Synergizing Algorithmic Design, Photoclick Chemistry and Multi‐Material Volumetric Printing for Accelerating Complex Shape Engineering

Advanced Science, Год журнала: 2023, Номер 10(26)

Опубликована: Июль 3, 2023

The field of biomedical design and manufacturing has been rapidly evolving, with implants grafts featuring complex 3D constraints materials distributions. By combining a new coding-based modeling approach high-throughput volumetric printing, is demonstrated to transform the way shapes are designed fabricated for applications. Here, an algorithmic voxel-based used that can generate large library porous structures, auxetic meshes cylinders, or perfusable constructs. deploying finite cell within framework, arrays selected designs be computationally modeled. Finally, schemes in conjunction approaches multi-material printing based on thiol-ene photoclick chemistry fabricate heterogeneous shapes. Collectively, design, fabrication techniques toward wide spectrum products such as actuators, grafts, tissue disease models.

Язык: Английский

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Lithography-based 3D printing of hydrogels

Nature Reviews Bioengineering, Год журнала: 2024, Номер unknown

Опубликована: Окт. 16, 2024

Язык: Английский

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Volumetric 3D Printing of Endoskeletal Soft Robots

Advanced Materials, Год журнала: 2024, Номер 36(33)

Опубликована: Июнь 14, 2024

Computed Axial Lithography (CAL) is an emerging technology for manufacturing complex parts, all at once, by circumventing the traditional layered approach using tomography. Overprinting, a unique additive capability of CAL, allows 3D geometry to be formed around prepositioned insert where occlusion light compensated other angular projections. This method opens door novel applications within multi-material systems such as endoskeletal robots. Herein, this work presents one application with simple Gelatin Methacrylate (GelMA)hydrogel osmotic actuator embedded system. GelMA ideal material it swellable and has reversible thermal gelation, enabling suspension endoskeleton during printing. By tuning formulation, design, post-processing, swelling-induced bending actuation 60 degrees achieved. To aid in printing process, computational determining absolute dose absorbed resin allowing print time prediction also proposed.

Язык: Английский

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