Cellular Trafficking of Nanotechnology‐Mediated mRNA Delivery

Advanced Materials, Год журнала: 2023, Номер 36(13)

Опубликована: Ноя. 6, 2023

Abstract Messenger RNA (mRNA)‐based therapy has emerged as a powerful, safe, and rapidly scalable therapeutic approach that involves technologies for both mRNA itself the delivery vehicle. Although there are some unique challenges different applications of therapy, common challenge all therapeutics is transport into target cell cytoplasm sufficient protein expression. This review focused on behaviors at cellular level nanotechnology‐mediated systems, which have not been comprehensively reviewed yet. First, four main introduced, including immunotherapy, replacement genome editing, reprogramming. Second, types cargos systems summarized. Third, strategies to enhance efficiency during trafficking process highlighted, accumulation cell, internalization endosomal escape, release from nanocarrier, translation protein. Finally, opportunities development presented. can provide new insights future fabrication nanocarriers with desirable performance.

Язык: Английский

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Theranostic Lipid Nanoparticles for Renal Cell Carcinoma

Advanced Materials, Год журнала: 2023, Номер unknown

Опубликована: Сен. 25, 2023

Abstract Renal cell carcinoma (RCC) is a common urological malignancy and represents leading threat to healthcare. Recent years have seen series of progresses in the early diagnosis management RCC. Theranostic lipid nanoparticles (LNPs) are increasingly becoming one focuses this field, because their suitability for tumor targeting multimodal therapy. LNPs can be precisely fabricated with desirable chemical compositions biomedical properties, which closely match physiological characteristics clinical needs Herein, comprehensive review theranostic presented, emphasizing generic tool nature developing advanced micro‐nano biomaterials. It begins brief overview formation mechanism LNPs, followed an introduction kidney‐targeting approaches, such as passive, active, stimulus responsive targeting. With examples provided, modification strategies enhancing functionality discussed. Thereafter, research advances on applications these RCC including bioimaging, liquid biopsy, drug delivery, physical therapy, gene therapy summarized discussed from interdisciplinary perspective. The final part highlights milestone achievements translation medicine, current challenges well future development directions treatment

Язык: Английский

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Unlocking the therapeutic potential of locked nucleic acids through lipid nanoparticle delivery

Molecular Therapy — Nucleic Acids, Год журнала: 2024, Номер 35(2), С. 102224 - 102224

Опубликована: Май 20, 2024

Locked nucleic acids (LNAs) are a subtype of antisense oligonucleotides (ASOs) that characterized by bridge within the sugar moiety. LNAs owe their robustness to this chemical modification, which as name suggests, locks it in one conformation. This perspective includes two components: general overview on ASOs from side and delivery issues focusing lipid nanoparticles (LNPs) other side. Throughout, screening ongoing clinical trials involving is given, well take versatility challenges using LNAs. Finally, we highlight potential LNPs carriers for successful

Язык: Английский

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Chimeric Antigen Cytotoxic Receptors for In Vivo Engineering of Tumor-Targeting NK Cells

ImmunoHorizons, Год журнала: 2024, Номер 8(1), С. 97 - 105

Опубликована: Янв. 1, 2024

Abstract Chimeric Ag receptor (CAR) NK cells are challenging to manufacture and fail achieve consistent tumor infiltration sustained cytolytic function in the microenvironment. In vivo engineering of using mRNA-based CAR delivery may overcome these issues. this study, we developed an programming method by designing CARs that leverage biology cell receptors for type–specific expression function. These were engineered fusion a recognition domain with natural cytotoxic family including NKp30, NKp44, NKp46. Our results demonstrated receptor–based can engage endogenous signaling adaptors effectively activate human lysis cytokine production. Specifically, discovered stable NKp44-based was contingent on presence immune cell–specific adaptor DAP12. This innovative strategy facilitates direct situ cells, enhancing safety minimizing off-target effects nontargeted, healthy tissues.

Язык: Английский

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Rational design of polymer-based mRNA delivery systems for cancer treatment

Polymer Chemistry, Год журнала: 2024, Номер 15(24), С. 2437 - 2456

Опубликована: Янв. 1, 2024

This review introduces the basic design principles and recent advances in polymeric mRNA therapeutics, highlighting strategies to realize cancer-selective, organ-targeted, tissue-penetrating delivery.

Язык: Английский

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