Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Дек. 3, 2024
The
antibiotic
resistance
genes
(ARGs)
limit
the
susceptibility
of
bacteria
to
antimicrobials,
representing
a
problem
high
importance.
Current
research
on
presence
ARGs
in
microorganisms
focuses
mainly
humans,
livestock,
hospitals,
or
wastewater.
However,
spectrum
dust
resistome
workplaces
and
households
has
gone
relatively
unexplored.
This
pilot
study
aimed
analyze
indoor
samples
from
participants'
(a
pediatric
hospital,
maternity
center)
compare
two
different
approaches
analysis;
high-throughput
quantitative
PCR
(HT-qPCR)
whole
metagenome
shotgun
sequencing
(WMGS).
In
total,
143
were
detected
using
HT-qPCR,
with
associated
macrolides,
lincosamides,
streptogramin
B
(MLSB)
phenotype
being
most
abundant,
followed
by
MDR
(multi-drug
resistance)
genes,
conferring
aminoglycosides.
A
higher
overall
relative
quantity
was
observed
than
households,
hospital
highest
ARGs.
WMGS
analysis
revealed
36
ARGs,
which
five
both
HT-qPCR
techniques.
Accordingly,
efficacy
approach
detect
lower
that
HT-qPCR.
summary,
our
data
buildings
where
people
spend
their
time
(workplaces,
households)
can
be
significant
source
antimicrobial-resistant
microorganisms,
may
potentially
pose
health
risk
humans
animals.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 28, 2025
Abstract
Despite
advances
in
dispersal
mechanisms
and
risk
assessment
of
antibiotic
resistance
genes
(ARGs),
how
plants
influence
ARG
contamination
agricultural
soils
remains
underexplored.
Here,
the
impacts
plant
species
diversity
on
ARGs
mobile
genetic
elements
(MGEs)
three
are
comprehensively
investigated
a
pot
experiment.
The
results
indicate
that
increased
reduces
MGEs
abundance
by
19.2%–51.2%,
whereas
exhibit
inconsistent
soil‐dependent
effects.
Potential
bacterial
hosts
harboring
abundant
have
greater
relative
than
nonhosts,
both
their
richness
cumulative
reduced
diversity.
Notably,
inhibited
present
other
hosts.
enriched
compounds
root
exudates
due
to
play
more
important
role
metabolic
network
contribute
rebalancing
potential
nonhosts.
An
independent
test
using
pure
organics
reveals
higher
resource
diversity,
resulting
from
mobility
high‐risk
ARGs.
This
study
highlights
resource‐mediated
mitigation
risks
posed
indicates
ensuring
is
promising
strategy
for
controlling
agroecosystems.
Abstract
Antibiotic
overuse
and
the
subsequent
environmental
contamination
of
residual
antibiotics
poses
a
public
health
crisis
via
an
acceleration
in
spread
antibiotic
resistance
genes
(ARGs)
through
horizontal
gene
transfer.
Although
occurrence,
distribution,
driving
factors
ARGs
soils
have
been
widely
investigated,
little
is
known
about
soilborne
pathogens
at
global
scale.
To
explore
this
gap,
contigs
from
1643
globally
sourced
metagnomes
are
assembled,
yielding
407
ARG‐carrying
(APs)
with
least
one
ARG;
APs
detected
1443
samples
(sample
detection
rate
87.8%).
The
richness
greater
agricultural
(with
median
20)
than
non‐agricultural
ecosystems.
Agricultural
possess
high
prevalence
clinical
affiliated
Escherichia,
Enterobacter,
Streptococcus
,
Enterococcus
.
tend
to
coexist
multidrug
bacA
A
map
soil
AP
generated,
where
anthropogenic
climatic
explained
hot
spots
East
Asia,
South
eastern
United
States.
results
herein
advance
understanding
distribution
determine
regions
prioritized
control
worldwide.
Global Change Biology,
Год журнала:
2024,
Номер
30(8)
Опубликована: Авг. 1, 2024
Human
activities
have
profoundly
altered
the
Earth's
phosphorus
(P)
cycling
process
and
its
associated
microbial
communities,
yet
their
global
distribution
pattern
response
to
human
influences
remain
unclear.
Here,
we
estimated
abundances
of
P-cycling
genes
from
3321
soil
metagenomic
samples
mapped
five
key
processes,
that
is,
organic
phosphoester
hydrolysis,
inorganic
solubilization,
two-component
system,
phosphotransferase
transporters.
Structural
equation
modeling
random
forest
analysis
were
employed
assess
impact
anthropogenic
environmental
factors
on
abundance
genes.
Our
findings
suggest
although
less
significant
than
climate
profile,
human-related
factors,
such
as
economic
population,
are
important
drivers
for
variations
in
gene
abundance.
Notably,
increased
parallel
extent
intervention,
but
generally
at
low
moderate
levels
activities.
Furthermore,
identified
critical
genera,
Pseudomonas
Lysobacter,
which
sensitive
changes
This
study
provides
insights
into
responses
microbes
a
scale,
enhancing
our
understanding
P
underscoring
importance
sustainable
biogeochemical
cycle.
Environment International,
Год журнала:
2024,
Номер
191, С. 108972 - 108972
Опубликована: Авг. 22, 2024
Overutilization
and
misuse
of
antibiotics
in
recent
decades
markedly
intensified
the
rapid
proliferation
diffusion
antibiotic
resistance
genes
(ARGs)
within
environment,
thereby
elevating
ARGs
to
status
a
global
public
health
crisis.
Recognizing
that
soil
acts
as
critical
reservoir
for
ARGs,
environmental
researchers
have
made
great
progress
exploring
sources,
distribution,
spread
soil.
However,
microscopic
state
micro-interfacial
behavior
remains
inadequately
understood.
In
this
study,
we
reviewed
behaviors
antibiotic-resistant
bacteria
(ARB)
porous
media,
predominantly
including
migration-deposition,
adsorption,
biofilm
formation.
Meanwhile,
proliferation,
degradation
were
identified
primary
soil,
with
component
serving
significant
determinant.
Our
work
contributes
further
comprehension
microstates
processes
ARB
environments
offers
theoretical
foundation
managing
mitigating
risks
associated
ARG
contamination.
Microorganisms,
Год журнала:
2024,
Номер
12(5), С. 911 - 911
Опубликована: Апрель 30, 2024
Microbial
communities
inhabiting
sedimentary
environments
in
river
source
regions
serve
as
pivotal
indicators
of
pristine
ecosystems.
While
the
correlation
between
antibiotic
resistome
and
pathogenicity
with
core
gut
bacteria
humans
is
well
established,
there
exists
a
significant
knowledge
gap
concerning
interaction
resistance
genes
(ARGs)
human
pathogenic
(HPB)
specific
microbes
basins,
often
referred
to
"terrestrial
gut".
Understanding
microbial
composition,
including
resident
genetic
elements
such
ARGs,
HPB,
Mobile
Genetic
Elements
(MGEs),
Virulence
Factors
(VFs),
within
natural
habitats
against
backdrop
global
change,
imperative.
To
address
this
gap,
an
enrichment-based
culturomics
complementary
along
metagenomics
was
conducted
study
characterize
biobank
provide
preliminary
ecological
insights
into
profiling
dissemination
ARGs
Lancang
River
Source
Basin.
Based
on
our
findings,
main
stream
Basin,
674
strains
bacteria,
comprising
540
under
anaerobic
conditions
124
aerobic
conditions,
were
successfully
isolated.
Among
these,
98
species
identified
known
species,
while
4
potential
novel
species.
Of
these
30
HPB
relevant
health.
Additionally,
