Programmable Enzymatic Reaction Network in Artificial Cell‐Like Polymersomes
Advanced Science,
Год журнала:
2024,
Номер
11(24)
Опубликована: Апрель 16, 2024
Abstract
The
ability
to
precisely
control
in
vitro
enzymatic
reactions
synthetic
cells
plays
a
crucial
role
the
bottom‐up
design
of
artificial
cell
models
that
can
recapitulate
key
cellular
features
and
functions
such
as
metabolism.
However,
integration
has
been
limited
bulk
or
microfluidic
emulsions
without
membrane,
lacking
more
sophisticated
higher‐order
communities
for
reconstituting
spatiotemporal
biological
information
at
multiple
length
scales.
Herein,
droplet
microfluidics
is
utilized
synthesize
cell‐like
polymersomes
with
distinct
molecular
permeability
driven
by
external
signals
fuels.
presence
competing
reverse
reaction
depletes
active
substrates
shown
enable
demonstration
fuel‐driven
formation
sub‐microcompartments
within
well
realization
out‐of‐equilibrium
systems.
In
addition,
different
characteristics
polymersome
membranes
are
exploited
successfully
construct
programmable
network
mimics
communication
heterogeneous
community
through
selective
transport.
Язык: Английский
Cell-free systems: A synthetic biology tool for rapid prototyping in metabolic engineering
Biotechnology Advances,
Год журнала:
2025,
Номер
unknown, С. 108522 - 108522
Опубликована: Янв. 1, 2025
Язык: Английский
Polymeric Giant Unilamellar Vesicles Support Longevity of Native Nuclei in Protocells
Small Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 18, 2025
Protocells
offer
a
versatile
material
for
dissecting
cellular
processes
and
developing
simplified
biomimetic
systems
by
combining
biological
components
with
synthetic
ones.
However,
gap
exists
between
the
integrity
complex
functionality
of
native
organelles
such
as
nuclei,
bottom‐up
strategies
reducing
functions
within
environment.
Here,
this
is
bridged
incorporating
nuclei
into
polymeric
giant
unilamellar
vesicles
(pGUVs)
using
double‐emulsion
microfluidics.
It
shown
that
retain
their
morphology
nuclear
envelope
integrity,
facilitating
import
co‐encapsulated
peptide‐based
multicompartment
micelles
(MCMs)
via
localization
signals
(NLS).
Importantly,
it
demonstrated
machinery
remains
functional
inside
protocells,
enriching
GUV
interior
import‐promoting
factors,
delivery
efficiency
NLS‐MCMs
significantly
increases.
The
findings
reveal
nucleated
protocells
preserve
function
extended
periods,
providing
new
platform
studying
in
simplified,
yet
biologically
relevant,
This
approach
opens
avenues
creating
advanced
biohybrid
materials,
offering
opportunities
to
investigate
organelle
behavior
interactions
greater
detail.
establish
foundation
high‐throughput
applications
biology
contribute
valuable
insights
sustaining
engineered
systems.
Язык: Английский
Advancements in Artificial Cell Preparation and Biomedical Applications
Macromolecular Bioscience,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 25, 2025
Artificial
cells
represent
a
transformative
innovation
in
biomedical
science,
offering
engineered,
cell-like
constructs
capable
of
mimicking
certain
biological
functions.
Although
remarkable
progress
has
been
made
to
construct
complex
multifunctional
artificial
cells,
there
are
still
significant
differences
between
and
natural
cells.
Thus,
thorough
understanding
the
techniques
challenges
associated
with
fabrication
alongside
their
applications,
is
essential
for
driving
further
technological
this
field.
This
review
aims
summarize
recent
advances
preparation
applications
In
emphasize
potential
revolutionize
cancer
therapeutics,
gene
therapy,
immunotherapy,
vaccine,
reactors
manufacture
biomolecules
signal
communication.
primarily
fabricated
through
bottom-up
approaches,
including
liposomes,
metal-organic
frameworks,
colloidal,
hydrogel,
droplet
microfluidic
which
allow
precise
control
over
composition,
structure,
functionality.
Finally,
future
outlook
development
simulating
real
activities
described.
Язык: Английский
Salt-Bridge-Mediated Coacervate-to-Vesicle Transformation in Arginine-Rich Coacervates
ACS Nano,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 22, 2025
Polypeptide-based
liquid-liquid
phase
separation
(LLPS)
has
received
considerable
attention
as
it
governs
the
formation
of
membraneless
organelles
in
cells.
However,
detailed
mechanistic
understanding
how
one
most
prevalent
cationic
amino
acids
proteins,
arginine,
interacts
with
various
biomolecules
to
induce
and
undergo
morphogenesis
remains
be
resolved.
Herein,
we
report
behavior
transformation
arginine-rich
coacervates
into
vesicular
structures
upon
introducing
polyphosphates.
Transformation
vesicles
was
shown
occur
independent
initial
anionic
counterparts
driven
by
salt-bridge
interactions
between
guanidinium
groups
arginine
residues
phosphates.
We
also
investigate
role
intermolecular
forces
ionic
effects
on
morphological
further
exploit
their
potential
assembly
artificial
tissue-like
constructs.
Overall,
our
findings
underpin
a
unifying
principle
for
vesicle
from
potency
reconstituting
hierarchical
biological
microcompartments.
Язык: Английский