Micropillars in Cell Mechanobiology: Design, Fabrication, Characterization, and Biosensing Applications

Small Science, Год журнала: 2024, Номер unknown

Опубликована: Дек. 9, 2024

Eukaryotic cells possess the remarkable ability to sense and respond mechanical cues from their extracellular environment, a phenomenon known as mechanobiology, which is crucial for proper functioning of biological systems. Micropillars have emerged prominent tool quantifying cellular forces demonstrated versatility beyond force measurement, including modulation environment facilitation mechano‐stimulation. In this comprehensive review, innovative strategies in micropillars’ design, fabrication, characterization, biosensing applications are explored. The review begins with foundational overview micropillar‐based cell mechanobiology studies provide complete understanding, then it delves into novel methodologies within each domain. latter part unveils such use enhancing surfaces upstream fluid manipulators biosensors. Finally, future research directions discussed current limitations these techniques outlined. Despite extensive exploration micropillar applications, significant gap remains between advancements practical implementation micropillars point‐of‐care diagnostics. Bridging translating laboratory innovations real‐world medical diagnostic tools.

Язык: Английский

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Enhanced electrophysiological activity and neurotoxicity screening of environmental chemicals using 3D neurons from human neural precursor cells purified with PSA-NCAM

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 280, С. 116516 - 116516

Опубликована: Май 31, 2024

The assessment of neurotoxicity for environmental chemicals is utmost importance in ensuring public health and safety. Multielectrode array (MEA) technology has emerged as a powerful tool assessing disturbances the electrophysiological activity. Although human embryonic stem cell (hESC)-derived neurons have been used MEA screening, obtaining substantial sufficiently active population from hESCs remains challenging. In this study, we successfully differentiated large neuronal precursor cells (hNPC) purified using polysialylated neural adhesion molecule (PSA-NCAM), referred to hNPC

Язык: Английский

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Laser-patterned epoxy-based 3D microelectrode arrays for extracellular recording

Nanoscale, Год журнала: 2024, Номер 16(30), С. 14295 - 14301

Опубликована: Янв. 1, 2024

Epoxy-based 3D microelectrode arrays facilitate extracellular recordings in non-planar environments.

Язык: Английский

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Inkjet‐Printed 3D Electrode Arrays for Recording Signals from Cortical Organoids

Advanced Materials Technologies, Год журнала: 2024, Номер 9(22)

Опубликована: Июль 19, 2024

Abstract Monitoring electrical activity across multiple planes in 3D cell cultures and organoids is imperative to comprehensively understand their functional connections behavior. However, traditional planar microelectrode arrays (MEAs) are intended for surface recordings inadequate addressing this aspect. The limitations, such as longer production times limited adaptability imposed by standard clean‐room techniques, constrain the design possibilities electrode potentially hinder effective cell‐electrode coupling. To tackle challenge, a novel approach presented that leverages rapid prototyping processes additive manufacturing combination with wet etching electrodeposition enhance fabrication performance. laser‐patterned MEAs on glass, polyimide (PI) foil, or polyethylene terephthalate (PET) foil substrates incorporate high‐aspect ratio (up 44:1) ink‐jet printed structures heights up 1 mm at pitch of 200 µm, enabling precise recording within tissues. specific shapes tips customizable provide great flexibility placement. versatility demonstrated electrophysiological cortical situ, paving way investigating neural under regular various pathologically altered conditions vitro high throughput manner.

Язык: Английский

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A Local De-insulation Method and Its Application in Neural Microneedle Array

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 8, 2024

Silicon-based neural microneedle arrays, such as the Utah Array, have demonstrated excellent performance in chronic recordings from cerebral cortex. Unlike planar thin-film electrodes with recording sites arranged on surface of a silicon film, arrays are located at tips three-dimensional needles, which significantly complicates fabrication process required for single-neuron recordings. To address this challenge, we develop local de-insulation method that eliminates need etching: encapsulated controllable-thickness protective layer, followed by deposition Parylene-C insulation layer. By optimizing adhesion and shape material both layer electrode shaft, were able to precisely control quality removed insulated layers, resulting consistent tip exposure. Experimental results show non-uniformity exposed silicon-based microelectrode (each has 10×10 array) fabricated using is 2.32 ± 0.57%, Furthermore, exhibited high stability reliability mechanical electrical characteristics. They achieving spike signal-to-noise ratio up 28 when tested vivo. This technique provides valuable development high-performance array.

Язык: Английский

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Multiphoton Polymerization-based Micro/Nanomanufacturing Toward Precision Medicine

Engineering, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

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Next-Generation Sensors with Three-Dimensional Micro-/Nano-Structures

Journal of Sensor Science and Technology, Год журнала: 2024, Номер 33(6), С. 419 - 428

Опубликована: Ноя. 30, 2024

Язык: Английский

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