Tissue and Cell, Год журнала: 2024, Номер 93, С. 102716 - 102716
Опубликована: Дек. 31, 2024
Язык: Английский
Advanced Science, Год журнала: 2024, Номер 11(24)
Опубликована: Апрель 19, 2024
Abstract Preventing and treating avascular necrosis at the distal end of flaps are critical to surgery success, but current treatments not ideal. A recent study shows that apoptotic bodies (ABs) generated near site apoptosis can be taken up promote cell proliferation. The reveals ABs derived from fibroblast‐like cells in subcutaneous connective tissue (FSCT cells) skin promoted ischaemic flap survival. It is also found inhibited death oxidative stress M1‐to‐M2 polarization macrophages. Transcriptome sequencing protein level testing demonstrated survival endothelial macrophages by inhibiting ferroptosis via KEAP1‐Nrf2 axis. Furthermore, microRNA (miR) data vitro vivo experiments KEAP1 delivering miR‐339‐5p exert therapeutic effects. In conclusion, FSCT cell‐derived ferroptosis, macrophage transition miR‐339‐5p/KEAP1/Nrf2 axis These results provide a potential strategy administering ABs.
Язык: Английский
Процитировано
18
ACS Nano, Год журнала: 2024, Номер 18(29), С. 19232 - 19246
Опубликована: Июль 12, 2024
Despite the superior efficacy of radiotherapy in esophageal squamous cell carcinoma (ESCC), radioresistance by cancer stem cells (CSCs) leads to recurrence, metastasis, and treatment failure. Therefore, it is necessary develop CSC-based therapies enhance radiotherapy. miR-339-5p (miR339) involved division DNA damage checkpoint signaling pathways based on ESCC cohort. miR339 inhibited stemness enhanced radiation-induced targeting USP8, suggesting that acts as a potential CSC regulator radiosensitizer. Considering limited circulating periods poor tumor-targeting ability miRNA, multifunctional nanoplatform bismuth sulfide nanoflower (Bi@PP) developed efficiently deliver improve radioresistance. Intriguingly, Bi@PP encapsulates more owing their flower-shaped structure, delivering than 1000-fold into cells, free alone. Besides being used carrier, advantageous for dynamically monitoring distribution delivered vivo while simultaneously inhibiting tumor growth. Additionally, Bi@PP/miR339 can significantly patient-derived xenograft models. This platform, incorporating higher miRNA loading capacity, pH responsiveness, hypoxia relief, CT imaging, provides another method promote radiosensitivity optimize treatment.
Язык: Английский
Процитировано
7
International Immunopharmacology, Год журнала: 2025, Номер 147, С. 114000 - 114000
Опубликована: Янв. 6, 2025
Язык: Английский
Процитировано
0
Biochemical Pharmacology, Год журнала: 2025, Номер 233, С. 116790 - 116790
Опубликована: Янв. 31, 2025
Язык: Английский
Процитировано
0
Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)
Опубликована: Фев. 4, 2025
A large body of evidence suggests that mesenchymal stromal cells (MSCs) are able to respond rapidly the cytokine milieu following systemic infusion. This encounter has potential dictate their therapeutic efficacy (also referred as licensing). MSCs react cellular damage by migrating inflamed tissue and ultimately modifying inflammatory microenvironment. However, limited use in clinical practice can be attributed a lack understanding fate patients after administration long term MSC-derived activity. While known physiological effectors viable make relative contribution, an innate property agent is caspase-dependent cell death. These mechanisms may involving functional reprogramming myeloid phagocytes via efferocytosis, process which apoptotic bodies (ABs) identified for engulfment both specialized non-specialized phagocytic cells. Recent studies have provided uptake ABs with distinct genetic component induce changes gene expression through epigenetic remodeling. phenomenon, 'trained immunity', significant impact on immunometabolism processes. It hypothesized diversity recipient within stroma adjacent potentially serve biomarker predicting outcome MSC treatment, while also contributing variable outcomes observed MSC-based therapies. Therefore, long-term reconstructive mediated apoptosis subsequent phagocyte-mediated efferocytosis.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)
Опубликована: Март 1, 2025
Abstract Background Chronic limb-threatening ischemia (CLTI) is the most severe form of peripheral arterial disease (PAD). Mesenchymal stem cell (MSC) transplantation holds promise as a treatment for CLTI; however, harsh local environment poses challenges to its effectiveness. Apoptotic vesicles (ApoVs) are extracellular produced by cells undergoing apoptosis, and they can carry various biomolecules from their parent cells, including proteins, RNA, DNA, lipids, ions, gas neurotransmitters. ApoVs play significant roles in anti-inflammatory responses, anti-tumor activities, tissue regeneration through intercellular communication, have demonstrated potential drug carriers. In this study, we investigated bone marrow (BMSC)-derived treating CLTI. Methods vivo, explored therapeutic effect on hindlimb model Laser Doppler, matrigel plug assay, histological analysis. vitro, analyzed effects proliferation, migration, angiogenesis HUVECs uptake process ApoVs. addition, Proteomic analysis, western blotting, quantitative real-time PCR, shRNA, siRNA were used analyze ApoVs-induced activation downstream signaling pathways. Results BMSCs showed improvement hind limb model, still exists after apoptosis BMSCs. Subsequently, isolated found improve mouse vivo. be ingested dynamin-, clathrin-, caveolin-mediated endocytosis promote angiogenesis. Mechanistically, transferred NAMPT HUVECs, therefore activating NAMPT/SIRT1/FOXO1 axis, influencing transcriptional activity FOXO1, promoting Conclusions Our results demonstrate that transplanted ameliorate releasing during apoptosis. The main mechanism axis. This study provides different insights into mechanisms suggests promising direction transplantation. Clinical trial number Not applicable. Graphical
Язык: Английский
Процитировано
0
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Март 21, 2025
Bionano robots have been recognized as a tumor-selective and effective platform for therapeutic outcomes they synergize the merits of living organisms nanoparticles. Here, we construct self-mineralized system (denoted SO@FeS) by employing facultative anaerobic bacterium Shewanella oneidensis MR-1 to biosynthesize FeS NPs cancer therapy with dual cell death pathways. Biogenic are embedded into surface inherent photothermal conversion ability low crystallinity tend simultaneously release Fe2+ hydrogen sulfide (H2S) in an acidic environment. As result, obtained SO@FeS hybrid can couple versatility nanoparticles respiration tumor-targeting capacities bacterium, ultimately leading collaborative clearance tumor cells. Specifically, cryo-soft X-ray tomography (cryo-SXT) is near-native 3D imaging modality that directly displays trafficking pathway More importantly, cryo-SXT captures maps SO@FeS-initiated ferroptosis apoptosis, evidenced remodeling cytoplasmic organelles. This work offers valuable theoretical insights from perspective organelle morphology, links subcellular reorganization pathways, facilitates design nanoplatforms integrate multiple therapies.
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 1, 2025
Abstract The process of apoptosis plays a crucial role in tissue homeostasis, immune system regulation, and organ formation. Apoptotic vesicles (ApoEVs) are involved efferocytosis, the by which phagocytes ingest dead cells. ApoEVs also have potential therapeutic applications cancer treatment, ischemic diseases, their anti-inflammatory properties make them incredibly versatile for medical applications. These can induce cells, provide tumor antigens vaccines, even serve as effective drug delivery systems. Moreover, they target hypoxic inhibit inflammatory cell death pathways, promote regeneration. Also, addressing disorders such gastrointestinal ailments, osteoarthritis, diabetes is promising. Additionally, polarize cells suppress responses viable option unmet need novel medications. Despite wealth reviews examining ApoEVs, very few thoroughly investigated mechanisms underlying effects. This distinctive approach positions current review timely immensely relevant, illuminating intriguing ways these entities function beyond established advantages. Graphical
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 7, 2025
In the field of large-area trauma flap transplantation, preventing avascular necrosis remains a critical challenge. Key mechanisms for improving viability include angiogenesis promotion, oxidative stress inhibition, and cell death prevention. Recently, two-dimensional ultrathin Ti3C2TX (MXene) nanosheets have gained attention their potential contributions to these processes, though MXene's physiological impact on survival had not been previously investigated. This study is first confirm biological effects ischaemic microenvironment post-skin transplantation. Findings indicated that MXene significantly decreased necrotic area in flaps (37.96% ± 2.00%), with reductions 30.40% 1.86% at 1 mg/mL 20.19% 2.11% 2 concentration-dependent manner. Mechanistically, facilitated situ angiogenesis, mitigated stress, suppressed pro-inflammatory pyroptosis, activated PI3K-Akt pathway, particularly influencing vascular endothelial cells. Comparative transcriptome analysis skin tissues without treatment provided additional evidence, highlighting such as ROS metabolic proliferation regulation, signaling pathway activation. Overall, demonstrated activity, effectively promoting presenting novel strategy addressing flaps.
Язык: Английский
Процитировано
0