Plasma-Activated Medium Inhibited the Proliferation and Migration of Non-Small Cell Lung Cancer A549 Cells in 3D Culture DOI Open Access

Zhidan Sun,

Chenglong Ding,

Yuhan Wang

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13262 - 13262

Опубликована: Дек. 10, 2024

Lung cancer is the most common type of malignant tumor worldwide. Plasma-activated medium (PAM) an innovative treatment method that has received considerable scientific attention. The objective this study to evaluate effects PAM on anti-tumor characteristics non-small cell lung (NSCLC) cells in two-dimensional (2D) and three-dimensional (3D) cultures. proliferative migratory capabilities A549 2D 3D cultures were assessed using MTT, migration, invasion assays, cycle, respectively. also investigated impact changes content intracellular extracellular reactive species analyzed protein expression Western Blot method. inhibited viability, abilities both cultures, suppressed epithelial–mesenchymal transition (EMT) process, downregulated RAS/ERK signaling pathway, which effectively spheroid formation. Additionally, effect was mediated through ROS-induced oxidative reactions, exhibited greater cytotoxicity culture compared culture. As 2D, model provides a viable vitro for studying mechanisms cancer. represents effective new NSCLC.

Язык: Английский

Reactive Oxygen Species‐Responsive Nanoparticles Toward Extracellular Matrix Normalization for Pancreatic Fibrosis Regression DOI Creative Commons
Liang Qi,

Bo‐Wen Duan,

Hui Wang

и другие.

Advanced Science, Год журнала: 2024, Номер 11(19)

Опубликована: Март 14, 2024

Abstract Pancreatic fibrosis (PF) is primarily characterized by aberrant production and degradation modes of extracellular matrix (ECM) components, resulting from the activation pancreatic stellate cells (PSCs) pathological cross‐linking ECM mediated lysyl oxidase (LOX) family members. The excessively deposited increases stiffness, over‐accumulated reactive oxygen species (ROS) induces oxidative stress, which further stimulates continuous PSCs advancing PF; challenging strategy toward normalizing homeostasis for regression PF. Herein, ROS‐responsive Vitamin A (VA) decorated micelles (named LR‐SSVA) to reverse imbalanced ameliorating PF are designed synthesized. Specifically, LR‐SSVA selectively targets via VA, thereby effectively delivering siLOXL1 resveratrol (RES) into pancreas. released RES inhibits overproduction eliminating ROS inactivating PSCs, meanwhile, decreased expression LOXL1 ameliorates cross‐linked collagen easier collagenase jointly normalizes alleviates This research shows that a safe efficient ROS‐response PSC‐targeted drug‐delivery system normalization, will propose an innovative ideal platform reversal

Язык: Английский

Процитировано

9

Pancreatic stellate cell: Update on molecular investigations and clinical translation in pancreatic cancer DOI Open Access
Yawei Liu, Ran Xue

International Journal of Cancer, Год журнала: 2025, Номер unknown

Опубликована: Янв. 18, 2025

Pancreatic cancer is a particularly aggressive tumor, distinguished by the presence of prominent collagenous stroma and desmoplasia that envelops tumor cells. stellate cell (PSC) contributes to formation dense fibrotic has been demonstrated facilitate progression. As significance PSCs increasingly revealed, more explorations are focused on complex molecular mechanisms tumor-stromal crosstalk in order guide potential therapeutic approaches through deactivating or reprogramming PSCs. Nevertheless, significant challenges persist translating preclinical discoveries into clinical applications. In this review, we expect offer comprehensive overview latest advancements PSCs, along with new insights strategies targeting

Язык: Английский

Процитировано

0

Fertile Ground for Homeostatic Reformation: Engineered Release Platforms for Enhanced Regenerative Outcomes DOI
Pei Wang, Yan Gong,

Xiansong Wang

и другие.

Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер 106, С. 106740 - 106740

Опубликована: Фев. 21, 2025

Язык: Английский

Процитировано

0

Piezoelectric nanofilms fabricated by coaxial electrospun polycaprolactone/Barium titanate promote Achilles tendon regeneration by reducing IL-17A/NF-κB-mediated inflammation DOI
Wufei Dai, Qi Xu, Qinglin Li

и другие.

Bioactive Materials, Год журнала: 2025, Номер 49, С. 1 - 22

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Engineered MSCs Break Endothelial‐Myofibroblast Crosstalk in Pulmonary Fibrosis: Reconstructing the Vascular Niche DOI

Yue‐Fei Fang,

Chen Zhang, Meng‐Meng Han

и другие.

Advanced Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Abstract In the progress of pulmonary fibrosis (PF), normal vascular niche plays a crucial role in alveolar regeneration by secreting angiocrine factors. However, malignant interaction between myofibroblasts and endothelial cells results significant loss capillaries fibroblast foci, which promotes continuous deterioration fibrosis. Herein, an engineered mesenchymal stem cell (MSC) therapeutic named MSC‐MM@LPHN is developed for reconstructing niche, formed modifying surface MSC with ROS‐responsive lipid polymeric hybrid nanoparticles encapsulating metformin macitentan. Due to homing ability MSC, can effectively target lung tissue, then induce myofibroblast dedifferentiation reduce secretion cytokines that cause damage preventing from turning into fibrotic phenotype, leading recovery function. Combined MSC‐secreted growth factors promoting angiogenesis, ultimately constructs structure area reverses bleomycin‐induced PF. The findings suggest targeting network treat PF, provides novel strategy fibrosis‐related diseases.

Язык: Английский

Процитировано

0

Bone marrow microenvironment-targeted yolk-shell nanoparticle enhances acute myeloid leukemia chemotherapy by preventing endothelial cell/ECM-mediated adhesion DOI

Shiyi Hong,

Peng Pei, Yutian Huang

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 164084 - 164084

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

Neutrophils inhibit bone formation by directly contacting osteoblasts and suppressing osteogenic differentiation DOI

Yijun Liu,

Fengyuan Guo,

Zhenshuo Han

и другие.

Bone, Год журнала: 2024, Номер 190, С. 117310 - 117310

Опубликована: Окт. 30, 2024

Язык: Английский

Процитировано

0

The Application of Nanomaterials in the Treatment of Pancreatic-Related Diseases DOI Open Access

Jing Ma,

Xue Li, Chunru Wang

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 13158 - 13158

Опубликована: Дек. 7, 2024

Pancreatic diseases, typically including pancreatic cancer, pancreatitis, and diabetes, pose enormous threats to people's lives health. To date, therapeutics with high therapeutic efficacy low side effects are still challenging. With the development of nanotechnology, nanomaterials have successfully been applied in pancretic disease treatment. Here, we first introduce diversity their different physicochemical properties on function. Following this, analyze potential enhance targeting by overcoming challenges traditional delivery methods through surface modifications, structural adjustments, optimized drug loading. Then, application structurally pancreatic-related diseases. For instance, cancer (as platforms, for promotion radiation therapy, as multifunctional tools), pancreatitis systems, anti-inflammatory anti-fibrotic agents), diabetes insulin carriers, protecting β cells, improving resistance). Through analysis progress current research, summarize how can treatment while minimizing effects. Finally, look forward prospects

Язык: Английский

Процитировано

0

Plasma-Activated Medium Inhibited the Proliferation and Migration of Non-Small Cell Lung Cancer A549 Cells in 3D Culture DOI Open Access

Zhidan Sun,

Chenglong Ding,

Yuhan Wang

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13262 - 13262

Опубликована: Дек. 10, 2024

Lung cancer is the most common type of malignant tumor worldwide. Plasma-activated medium (PAM) an innovative treatment method that has received considerable scientific attention. The objective this study to evaluate effects PAM on anti-tumor characteristics non-small cell lung (NSCLC) cells in two-dimensional (2D) and three-dimensional (3D) cultures. proliferative migratory capabilities A549 2D 3D cultures were assessed using MTT, migration, invasion assays, cycle, respectively. also investigated impact changes content intracellular extracellular reactive species analyzed protein expression Western Blot method. inhibited viability, abilities both cultures, suppressed epithelial–mesenchymal transition (EMT) process, downregulated RAS/ERK signaling pathway, which effectively spheroid formation. Additionally, effect was mediated through ROS-induced oxidative reactions, exhibited greater cytotoxicity culture compared culture. As 2D, model provides a viable vitro for studying mechanisms cancer. represents effective new NSCLC.

Язык: Английский

Процитировано

0