International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(24), С. 13706 - 13706
Опубликована: Дек. 22, 2024
Single-cell
sequencing
technology
provides
apparent
advantages
in
cell
population
heterogeneity,
allowing
individuals
to
better
comprehend
tissues
and
organs.
Sequencing
is
currently
moving
beyond
the
standard
transcriptome
single-cell
level,
which
likely
bring
new
insights
into
function
of
breast
cells.
In
this
study,
we
examine
primary
types
involved
development,
as
well
achievements
study
scRNA-seq
microenvironment,
stressing
finding
novel
subsets
using
approaches
analyzing
problems
solutions
scRNA-seq.
Furthermore,
are
excited
about
field’s
promising
future.
Abstract
Single‐cell
multiomic
and
exosome
analyses
are
potent
tools
in
various
fields,
such
as
cancer
research,
immunology,
neuroscience,
microbiology,
drug
development.
They
facilitate
the
in‐depth
exploration
of
biological
systems,
providing
insights
into
disease
mechanisms
aiding
treatment.
isolation,
which
is
crucial
for
single‐cell
analysis,
ensures
reliable
cell
isolation
quality
control
further
downstream
analyses.
Microfluidic
chips
small
lightweight
systems
that
efficient
high‐throughput
real‐time
analysis
on‐
or
off‐chip.
Therefore,
most
current
technologies
based
on
microfluidic
technology.
This
review
offers
comprehensive
guidance
to
researchers
across
different
fields
selection
appropriate
chip
analysis.
describes
design
principles,
separation
mechanisms,
characteristics,
cellular
effects
available
isolation.
Moreover,
this
highlights
implications
using
technology
subsequent
analyses,
including
Finally,
challenges
future
prospects
outlined
multiplex
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Abstract
Microfluidic
droplets,
with
their
unique
properties
and
broad
applications,
are
essential
in
chemical,
biological,
materials
synthesis
research.
Despite
the
flourishing
studies
on
artificial
intelligence‐accelerated
microfluidics,
most
research
efforts
have
focused
upstream
design
phase
of
microfluidic
systems.
Generating
user‐desired
droplets
still
remains
laborious,
inefficient,
time‐consuming.
To
address
long‐standing
challenges
associated
accurate
efficient
identification,
sorting,
analysis
morphology
generation
rate
single
double
emulsion
a
novel
machine
vision
approach
utilizing
deformable
detection
transformer
(DETR)
algorithm
is
proposed.
This
method
enables
rapid
precise
(detection
relative
error
<
4%
precision
>
94%)
across
various
scales
scenarios,
including
real‐world
simulated
environments.
identification
(MDIA),
web‐based
tool
powered
by
Deformable
DETR,
which
supports
transfer
learning
to
enhance
accuracy
specific
user
scenarios
developed.
MDIA
characterizes
diameter,
number,
frequency,
other
parameters.
As
more
training
data
added
users,
MDIA's
capability
universality
expand,
contributing
comprehensive
database
for
droplet
microfluidics.
The
work
highlights
potential
intelligence
advancing
regulation,
fabrication,
label‐free
analysis,
accelerating
biochemical
sciences
engineering.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 31, 2025
Abstract
The
early
detection
of
high‐risk
human
papillomavirus
(HR‐HPV)
is
crucial
for
the
assessment
and
improvement
prognosis
in
cervical
cancer.
However,
existing
PCR‐based
screening
methods
suffer
from
inadequate
accessibility,
which
dampens
enthusiasm
among
grassroots
populations,
especially
resource‐limited
areas,
contributes
to
persistently
high
mortality
rate
Here,
a
portable
system
proposed
multiplexed
nucleic
acid
detection,
termed
R‐CHIP
,
that
integrates
R
ecombinase
polymerase
amplification
(RPA),
C
RISPR
H
and‐driven
microfluidics,
an
artificial
I
ntelligence
P
latform.
can
go
sample
pre‐processing
results
readout
less
than
hour
with
simple
manual
operation.
Optimized
sensitivity
10
−17
M
HPV‐16
−18
HPV‐18,
has
accuracy
over
95%
300
tests
on
clinical
samples.
In
addition,
smartphone
microimaging
combined
ResNet‐18
deep
learning
model
used
improve
efficiency
convenience
system,
initial
prediction
accuracies
96.0%
98.0%
respectively.
R‐CHIP,
as
user‐friendly
intelligent
platform,
great
potential
community‐level
HR‐HPV
resource‐constrained
settings,
prevention
diagnosis
other
diseases.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 20, 2025
Microfluidics
is
an
emerging
technology
for
buffer
exchange
in
bioprocessing
applications.
However,
achieving
with
simplicity
of
operation
and
high
throughput
a
straightforward
channel
design
remains
challenge.
This
study
presents
novel
semicircular
microchannel
that
allows
the
deterministic
regulation
helical
Dean
vortices
through
geometric
confinement.
By
incorporating
micro-obstacles
into
microchannels
large
dimensions
(900
μm
wide
100
high),
we
observe
substantial
enhancement
secondary
flows,
leading
to
unique
fluid
distribution
across
range
flow
rates.
enables
particle
separation
efficiency
(>96.27%)
coupled
low
fluorescein
purity
(<4.46%)
at
3
×
106
particles/min.
The
proposed
methodology,
characterized
by
ease
production
(simple
dimensions),
user-friendly
(uniform
rates
both
sheath
sample
inlets),
efficient
capabilities
(typically
mL
min–1),
demonstrates
significant
potential
advancing
microfluidic
systems
biological
biomedical
research.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 10, 2025
This
work
developed
a
cell
damage
recognition
method
based
on
single-cell
arrays
using
an
artificial
intelligence
tool.
The
uses
micropatterns
(single-cell
micropatches
and
microwells)
to
isolate
each
in
ordered
array
minimize
overlapping
maintain
contours.
After
exposure
therapeutic
drug
(e.g.,
doxorubicin),
large
number
of
single
cells
are
monitored,
the
levels
determined
with
both
morphology
intensity
changes
reactive
oxygen
species
recorded
under
fluorescence
microscopy.
convolutional
neural
network
model
is
trained
by
time-series
cancer
images
before
after
low
high
concentrations
exposure.
can
identify
status
(live/dead)
classify
(major/moderate/minor)
accuracy.
pattern
allows
physically
segmented
at
level,
which
not
only
eliminates
need
for
computational
segmentation
but
also
reduces
background
noise
neighboring
interference,
highly
enhances
accuracy
analysis
via
image
recognition.
accelerates
toxicity
AI
model,
be
used
predict
response
screening
potential
anticancer
drugs.
Biomolecules,
Год журнала:
2025,
Номер
15(3), С. 360 - 360
Опубликована: Март 1, 2025
This
article
reviews
the
current
applications
of
digital
polymerase
chain
reaction
(dPCR)
in
non-invasive
prenatal
testing
(NIPT)
and
explores
its
potential
to
complement
or
surpass
capabilities
Next-Generation
Sequencing
(NGS)
testing.
The
growing
incidence
genetic
disorders
maternal–fetal
medicine
has
intensified
demand
for
precise
accessible
NIPT
options,
which
aim
minimize
need
invasive
diagnostic
procedures.
Cell-free
fetal
DNA
(cffDNA),
core
analyte
NIPT,
is
influenced
by
numerous
factors
such
as
maternal
contamination,
placental
health,
fragment
degradation.
dPCR,
with
inherent
precision
ability
detect
low-abundance
targets,
demonstrates
robustness
against
these
interferences.
Although
NGS
remains
gold
standard
due
comprehensive
capabilities,
high
costs
limit
widespread
use,
particularly
resource-limited
settings.
In
contrast,
dPCR
provides
comparable
accuracy
lower
complexity
expense,
making
it
a
promising
alternative
Biomedical Microdevices,
Год журнала:
2025,
Номер
27(1)
Опубликована: Март 1, 2025
Abstract
Total
internal
reflection
fluorescence
(TIRF)
microscopy
is
a
powerful
imaging
technique
that
visualizes
the
outer
surface
of
specimens
in
close
proximity
to
substrate,
yielding
crucial
insights
cell
membrane
compositions.
TIRF
plays
key
role
single-cell
studies
but
typically
requires
chemical
fixation
ensure
direct
contact
between
and
which
can
compromise
viability
promote
clustering.
In
this
study,
we
present
microfluidic
device
with
structures
designed
trap
single
yeast
cells
fix
them
substrate
enable
measurements
on
membrane.
The
traps
are
fabricated
using
two-photon
polymerization,
allowing
high-resolution
printing
intricate
encapsulate
all
three
dimensions
while
maintaining
exposure
flow
within
device.
Our
adaptable
design
allows
us
reduce
residual
movement
trapped
minimum
high
trapping
efficiencies.
We
identify
optimal
structure
configuration
demonstrate
efficiency
be
tuned
by
modifying
concentration
injection
methods.
Additionally,
replicating
soft
hydrogel
materials,
potential
our
approach
for
further
studies.
authors
have
no
relevant
financial
or
non-financial
interests
disclose
competing
declare.
Graphical
abstract
