ACS Applied Engineering Materials, Год журнала: 2024, Номер unknown
Опубликована: Дек. 4, 2024
Язык: Английский
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Янв. 13, 2025
Optical metasurfaces, arrays of nanostructures engineered to manipulate light, have emerged as a transformative technology in both research and industry due their compact design exceptional light control capabilities. Their strong light–matter interactions enable precise wavefront modulation, polarization control, significant near-field enhancements. These unique properties recently driven application biomedical fields. In particular, metasurfaces led breakthroughs imaging technologies, such achromatic imaging, phase extended depth-of-focus imaging. They also advanced cutting-edge biosensing featuring high-quality factor resonators As the demand for device miniaturization system integration increases, are expected play pivotal role development next-generation devices. this review, we explore latest advancements use applications, with particular focus on sensing. Additionally, discuss future directions aimed at transforming field by leveraging full potential provide compact, high-performance solutions wide range applications.
Язык: Английский
Процитировано
10
ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown
Опубликована: Янв. 29, 2025
Traditional cell culture methods face significant limitations in monitoring secretions with spatial and temporal precision. Advanced microsystems incorporating biosensors have been developed to address these challenges, but they tend lack versatility, their complexity, along the requirement for specialized equipment, limits broader adoption. CellStudio offers an innovative, user-friendly solution that exploits Printing Vacuum Lithography combined bead-based assays create modular tunable patterns surrounded by biosensors. This platform allows high-resolution, spatially resolved analysis of secreted proteins, such as VEGF FGF-2, while being easily implementable standard laboratory settings. CellStudio's design is compatible conventional facilitating its integration into existing workflows without need extensive training or tools. Validation experiments using mesenchymal stem cells HeLa demonstrated can detect small secretion levels from clusters high sensitivity analyze diffusion profiles, remarking possibilities studying behavior. By offering a standardized, cost-effective approach detailed cellular analysis, significantly enhances capabilities traditional techniques broad applications across biological biomedical research.
Язык: Английский
Процитировано
1
MedComm, Год журнала: 2024, Номер 5(10)
Опубликована: Сен. 21, 2024
Organoids are miniature, highly accurate representations of organs that capture the structure and unique functions specific organs. Although field organoids has experienced exponential growth, driven by advances in artificial intelligence, gene editing, bioinstrumentation, a comprehensive overview organoid applications remains necessary. This review offers detailed exploration historical origins characteristics various types, their applications-including disease modeling, drug toxicity efficacy assessments, precision medicine, regenerative medicine-as well as current challenges future directions research. have proven instrumental elucidating genetic cell fate hereditary diseases, infectious metabolic disorders, malignancies, study processes such embryonic development, molecular mechanisms, host-microbe interactions. Furthermore, integration technology with intelligence microfluidics significantly advanced large-scale, rapid, cost-effective thereby propelling progress medicine. Finally, advent high-performance materials, three-dimensional printing technology, also gaining prominence Our insights predictions aim to provide valuable guidance researchers support continued advancement this rapidly developing field.
Язык: Английский
Процитировано
9
Advanced Science, Год журнала: 2024, Номер unknown
Опубликована: Июнь 24, 2024
Organoid tumor models have emerged as a powerful tool in the fields of biology and medicine such 3D structures grown from cells recapitulate better characteristics, making these tumoroids unique for personalized cancer research. Assessment their functional behavior, particularly protein secretion, is significant importance to provide comprehensive insights. Here, label-free spectroscopic imaging platform presented with advanced integrated optofluidic nanoplasmonic biosensor that enables real-time secretion analysis single tumoroids. A novel two-layer microwell design isolates tumoroids, preventing signal interference, microarray configuration allows concurrent multiple The dual capability combining time-lapse plasmonic spectroscopy bright-field microscopy facilitates simultaneous observation dynamics, motility, morphology. demonstrated colorectal derived both cell lines patient samples investigate vascular endothelial growth factor (VEGF-A) growth, movement under various conditions, including normoxia, hypoxia, drug treatment. This platform, by offering approach nanophotonics monitor can pave way new applications fundamental biological studies, screening, development therapies.
Язык: Английский
Процитировано
8
Research, Год журнала: 2024, Номер 8
Опубликована: Дек. 17, 2024
Recent advancements in nanotechnology have revolutionized terahertz (THz) technology. By enabling the creation of compact, efficient devices through nanoscale structures, such as nano-thick heterostructures, metasurfaces, and hybrid systems, these innovations offer unprecedented control over THz wave generation modulation. This has led to substantial enhancements spectroscopy, imaging, especially bio-applications, providing higher resolution sensitivity. review comprehensively examines latest nanoengineered technology, beginning with state-of-the-art methods based on followed by modulation techniques, including both homogeneous individual Subsequently, it explores bio-applications novel biosensing biofunction techniques. Finally, summarizes findings reflects future trends challenges field. Each section focuses physical mechanisms, structural designs, performances, aiming provide a thorough understanding potential this rapidly evolving technology domain. aims insights into next-generation applications while establishing comprehensive foundation for addressing key issues that limit full implementation promising technologies real-world scenarios.
Язык: Английский
Процитировано
3
Cell Death Discovery, Год журнала: 2025, Номер 11(1)
Опубликована: Май 7, 2025
Язык: Английский
Процитировано
0
Small Methods, Год журнала: 2025, Номер unknown
Опубликована: Май 28, 2025
Abstract Advances in organoid models, as ex vivo mini‐organs, and the development of screening imaging technologies have continuously driven each other forward. A complete understanding organoids requires detailed insights into intertwined intraorganoid extraorganoid activities how they change across time space. This study introduces a multiplexed platform that integrates label‐free nanoplasmonic biosensing with fluorescence microscopy to offer simultaneous monitoring dynamics occurring within around arrays single spheroids spatiotemporal resolution. The module employs biosensors extraordinary optical transmission track biomolecular secretions surroundings, while concurrent enables structural analysis viability assessment. To perform multiparametric interrogation data from different channels over extended periods, deep‐learning‐augmented image is incorporated. applied tumor investigate vascular endothelial growth factor secretion alongside morphometric changes viability, showcasing its ability capture variations between untreated drug‐treated groups. integrated approach advances comprehensive models can complement existing accelerate discoveries disease modeling drug development.
Язык: Английский
Процитировано
0
ACS Applied Engineering Materials, Год журнала: 2024, Номер unknown
Опубликована: Дек. 4, 2024
Язык: Английский
Процитировано
0