Abstract
With
the
rapid
advancement
of
DNA
technology,
intelligent
nanoreactors
(iDNRs)
have
emerged
as
sophisticated
tools
that
harness
structural
versatility
and
programmability
DNA.
Due
to
their
functional
programmability,
iDNRs
play
an
important
unique
role
in
vivo
tumor
diagnosis
therapy.
This
review
provides
overview
design
methods
for
based
on
advanced
including
enzymatic
reaction‐mediated
enzyme‐free
strategies.
also
focuses
how
achieve
intelligence
through
design,
well
applications
In
summary,
this
summarizes
current
advances
discusses
existing
challenges,
proposes
future
directions
expanding
applications,
which
are
expected
provide
insights
into
development
field
diagnostics
targeted
therapies.
Journal of Chemical Information and Modeling,
Год журнала:
2024,
Номер
64(21), С. 8339 - 8348
Опубликована: Окт. 18, 2024
DNA's
ability
to
exist
in
a
wide
variety
of
structural
forms,
subforms,
and
secondary
motifs
is
fundamental
numerous
biological
processes
has
driven
the
development
biotechnological
applications.
Major
determinants
DNA
flexibility
are
multiple
torsional
degrees
freedom
around
phosphodiester
backbone.
This
high
complexity
can
be
rationalized
by
using
two
pseudotorsional
angles
linking
atoms
P
C4′,
from
which
Ramachandran-like
plots
built.
In
this
contribution,
we
explore
distribution
η
(eta:
C4′i–1-Pi-C4′i-Pi+1)
θ
(theta:
Pi-C4′i-Pi+1-C4′i+1)
known
experimental
structures
retrieved
Protein
Data
Bank
(PDB),
subdividing
conformational
space
into
different
datasets.
After
removal
canonical/helical
conformations
typical
B-form,
find
existence
map
with
clearly
permitted
forbidden
regions.
Some
these
regions
populated
specific
like
Z-
or
A-DNA,
motifs,
G-quadruplexes
junctions.
We
evaluated
sequence
dependency
energy
relationship
among
high-density
identified
η–θ
space.
Furthermore,
analyzed
effect
produced
proteins
cations
when
bound
DNA,
finding
that
produce
some
nonhelical
conformations,
while
other
appear
stabilized
divalent
cations.
Pharmaceutics,
Год журнала:
2024,
Номер
16(11), С. 1434 - 1434
Опубликована: Ноя. 11, 2024
Targeted
delivery
of
chemotherapeutic
agents
is
a
well-established
approach
to
cancer
therapy.
Antibody-drug
conjugates
(ADCs)
typically
carry
toxic
payloads
attached
tumor-associated
antigen-targeting
IgG
antibody
via
an
enzyme-cleavable
linker
that
releases
the
drug
inside
cell.
Aptamers
are
promising
alternative
antibodies
in
terms
antigen
targeting;
however,
their
polynucleotide
nature
and
smaller
size
result
completely
different
PK/PD
profile
compared
IgG.
This
may
prove
advantageous:
owing
lower
molecular
weight,
aptamer-drug
achieve
better
penetration
solid
tumors
ADCs.
