Modular Fabrication of Microfluidic Graphene FET for Nucleic Acids Biosensing
Qiongdi Zhang,
Yuxuan Hao,
Tonghua Zeng
и другие.
Advanced Science,
Год журнала:
2024,
Номер
11(39)
Опубликована: Июль 23, 2024
Graphene
field-effect
transistors
(GFETs)
are
widely
used
in
biosensing
due
to
their
excellent
properties
biomolecular
signal
amplification,
exhibiting
great
potential
for
high-sensitivity
and
point-of-care
testing
clinical
diagnosis.
However,
difficulties
complicated
fabrication
steps
the
main
limitations
further
studies
applications
of
GFETs.
In
this
study,
a
modular
technique
is
introduced
construct
microfluidic
GFET
biosensors
within
3
independent
steps.
The
low-melting
metal
electrodes
intricate
flow
channels
incorporated
maintain
structural
integrity
graphene
facilitate
subsequent
sensing
operations.
as-fabricated
biosensor
demonstrates
long-term
stability,
performs
effectively
various
ion
environments.
It
also
exhibits
high
sensitivity
selectivity
detecting
single-stranded
nucleic
acids
at
10
fm
concentration.
Furthermore,
when
combined
with
CRISPR/Cas12a
system,
it
facilitates
amplification-free
rapid
detection
concentration
1
fm.
Thus,
believed
that
modular-fabricated
may
shed
light
on
development
FET-based
applications.
Язык: Английский
Amplification-free nucleic acids detection with next-generation CRISPR/dx systems
Critical Reviews in Biotechnology,
Год журнала:
2024,
Номер
unknown, С. 1 - 28
Опубликована: Сен. 22, 2024
CRISPR-based
diagnostics
(CRISPR/Dx)
have
revolutionized
the
field
of
molecular
diagnostics.
It
enables
home
self-test,
field-deployable,
and
point-of-care
testing
(POCT).
Despite
great
potential
CRISPR/Dx
in
diagnoses
biologically
complex
diseases,
preamplification
template
often
is
required
for
sensitive
detection
low-abundance
nucleic
acids.
Various
amplification-free
systems
were
recently
developed
to
enhance
signal
at
sufficient
sensitivity.
Broadly,
these
are
classified
into
five
groups
depending
on
enhancement
strategies
employed:
CRISPR/Cas12a
and/or
CRISPR/Cas13a
integrated
with:
(1)
other
catalytic
enzymes
(Cas14a,
Csm6,
Argonaute,
duplex-specific
nuclease,
nanozyme,
or
T7
exonuclease),
(2)
rational-designed
oligonucleotides
(multivalent
aptamer,
tetrahedral
DNA
framework,
RNA
G-quadruplexes,
roller
machine,
switchable-caged
guide
RNA,
hybrid
locked
RNA/DNA
probe,
hybridized
cascade
"U"
rich
stem-loop
RNA),
(3)
nanomaterials
(nanophotonic
structure,
gold
nanoparticle,
micromotor,
microbeads),
(4)
electrochemical
piezoelectric
plate
biosensors
(SERS
nanoprobes,
graphene
field-effect
transistor,
redox
primer
exchange
reaction),
(5)
cutting-edge
technology
platforms
(digital
bioanalysis,
droplet
microfluidic,
smartphone
camera,
single
nanoparticle
counting).
Herein,
we
critically
discuss
advances,
pitfalls
future
perspectives
acids
detection.
The
continued
refinement
will
pave
road
rapid,
cost-effective,
ultrasensitive,
ultraspecific
on-site
without
resorting
target
amplification,
with
ultimate
goal
establishing
as
paragon
Язык: Английский
Nanomaterials-Integrated CRISPR/Cas Systems: Expanding the Toolbox for Optical Detection
ACS Sensors,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 9, 2025
Nanomaterials-integrated
CRISPR/Cas
systems
have
rapidly
emerged
as
powerful
next-generation
platforms
for
optical
biosensing.
These
integrated
harness
the
precision
of
CRISPR/Cas-mediated
nucleic
acid
detection
while
leveraging
unique
properties
nanomaterials
to
achieve
enhanced
sensitivity
and
expanded
analytical
capabilities,
thereby
broadening
their
diagnostic
potential.
By
incorporating
a
diverse
range
nanomaterials,
these
effectively
expand
toolbox
detection,
offering
adaptable
solutions
tailored
various
challenges.
This
review
provides
comprehensive
overview
successfully
into
CRISPR/Cas-based
sensing
systems.
It
examines
multiple
modalities,
including
fluorescence,
electrochemiluminescence,
colorimetry,
surface-enhanced
Raman
spectroscopy,
highlighting
how
facilitate
signal
amplification,
enable
multiplexing,
support
development
point-of-care
applications.
Additionally,
practical
applications
in
critical
fields
such
healthcare
diagnostics
environmental
monitoring
are
showcased.
While
offer
considerable
advantages,
several
real-world
challenges
complexity
assay
workflows,
impact
cost,
regulatory
hurdles
must
be
addressed
before
widespread
implementation
can
achieved.
identifying
obstacles
proposing
strategic
solutions,
we
aim
pave
way
continued
advancement
adoption
nanomaterial-integrated
biosensing
technologies.
Язык: Английский
Multifunctional Integrated Biosensors Based on Two-Dimensional Field-Effect Transistors
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(51), С. 70160 - 70173
Опубликована: Дек. 11, 2024
In
recent
years,
field-effect
transistor
(FET)
sensing
technology
has
attracted
significant
attention
owing
to
its
noninvasive,
label-free,
real-time,
and
user-friendly
detection
capabilities.
Owing
the
large
specific
surface
area,
high
flexibility,
excellent
conductivity
of
two-dimensional
(2D)
materials,
FET
biosensors
based
on
2D
materials
have
demonstrated
unique
potential
in
biomarker
analysis
healthcare
applications,
driving
continuous
innovation
transformation
field.
Here,
we
review
trends
development
key
performance
metrics
main
characteristics,
also
discuss
structural
designs
modification
strategies
for
biosensing
devices
utilizing
graphene,
transition
metal
dichalcogenides,
black
phosphorus,
other
enhance
metrics.
Finally,
offer
insights
into
future
directions
biosensor
advancements,
improvements,
present
new
recommendations
practical
clinical
applications.
Язык: Английский