Zwitterion‐Lubricated Hydrogel Microspheres Encapsulated with Metformin Ameliorate Age‐Associated Osteoarthritis
Advanced Science,
Год журнала:
2024,
Номер
11(30)
Опубликована: Июнь 14, 2024
Chondrocyte
senescence
and
reduced
lubrication
play
pivotal
roles
in
the
pathogenesis
of
age-related
osteoarthritis
(OA).
In
present
study,
highly
lubricated
drug-loaded
hydrogel
microspheres
are
designed
fabricated
through
radical
polymerization
sulfobetaine
(SB)-modified
hyaluronic
acid
methacrylate
using
microfluidic
technology.
The
copolymer
contains
a
large
number
SB
carboxyl
groups
that
can
provide
high
degree
hydration
form
electrostatic
loading
interactions
with
metformin
(Met@SBHA),
producing
drug
load
for
anti-chondrocyte
senescence.
Mechanical,
tribological,
release
analyses
demonstrated
enhanced
lubricative
properties
prolonged
dissemination
Met@SBHA
microspheres.
RNA
sequencing
(RNA-seq)
analysis,
network
pharmacology,
vitro
assays
revealed
extraordinary
capacity
to
combat
chondrocyte
Additionally,
inducible
nitric
oxide
synthase
(iNOS)
has
been
identified
as
promising
protein
modulated
by
Met
senescent
chondrocytes,
thereby
exerting
significant
influence
on
iNOS/ONOO-/P53
pathway.
Notably,
intra-articular
administration
aged
mice
ameliorated
cartilage
OA
pathogenesis.
Based
findings
this
emerges
an
innovative
strategy
tackling
serving
dual
function
enhancing
joint
mitigating
Язык: Английский
Preparation of hydrogel microsphere and its application in articular cartilage injury
Materials Today Bio,
Год журнала:
2025,
Номер
31, С. 101641 - 101641
Опубликована: Март 8, 2025
In
recent
years,
hydrogel
microspheres
have
garnered
significant
attention
due
to
their
unique
structure
and
functionality,
demonstrating
substantial
potential
in
articular
cartilage
injury
repair.
This
paper
provides
a
comprehensive
overview
of
current
strategies
for
repair
summarizes
the
materials
preparation
methods
microspheres.
Furthermore,
it
highlights
multiple
roles
repair,
including
inflammation
control,
regulation
chondrocyte
metabolism,
drug
cell
delivery,
lubrication
improvement,
recruitment
endogenous
stem
cells.
Finally,
discusses
application
prospects
microspheres,
identifies
limitations
challenges,
offers
insights
guide
future
research
practical
applications
Язык: Английский
Targeting p21‐Positive Senescent Chondrocytes via IL‐6R/JAK2 Inhibition to Alleviate Osteoarthritis
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 23, 2025
Abstract
Osteoarthritis
(OA)
is
an
age‐related
degenerative
joint
disease,
prominently
influenced
by
the
pro‐inflammatory
cytokine
interleukin‐6
(IL‐6).
Although
elevated
IL‐6
levels
in
fluid
are
well‐documented,
uneven
cartilage
degeneration
observed
knee
OA
patients
suggests
additional
underlying
mechanisms.
This
study
investigates
role
of
receptor
(IL‐6R)
mediating
signaling
and
its
contribution
to
progression.
Here,
significantly
IL‐6R
expression
identified
degenerated
patients.
Further,
vivo
experiments
reveal
that
intra‐articular
injection
recombinant
protein
or
activation
gp130
(Y757F
mutation)
accelerates
Conversely,
knockout
JAK2,
as
well
treatment
with
a
JAK
inhibitor,
alleviates
symptoms.
Mechanistically,
chondrocytes
derived
from
exhibit
impaired
nuclear
localization
SOX9,
key
regulator
homeostasis.
inhibition
stabilizes
SIRT1,
reduces
SOX9
acetylation,
thereby
facilitates
localization,
promoting
repair.
Additionally,
inhibitor‐induced
apoptosis
p21‐positive
senescent
cells,
their
targeted
clearance
successfully
p21‐3MR
mice.
In
conclusion,
these
findings
novel
mechanism
which
inhibiting
IL‐6R/JAK2
pathway
can
alleviate
OA.
Furthermore,
this
proposes
targeting
cells
new
therapeutic
strategy
for
Язык: Английский
Hydrogels as drug delivery platforms for orthopedic diseases treatment: A review
International Journal of Biological Macromolecules,
Год журнала:
2025,
Номер
unknown, С. 140902 - 140902
Опубликована: Фев. 1, 2025
Язык: Английский
A Core‐Brush Nanoplatform with Enhanced Lubrication and Anti‐Inflammatory Properties for Osteoarthritis Treatment
Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 1, 2024
Abstract
Osteoarthritis
(OA)
is
recognized
as
a
highly
friction‐related
joint
disease
primarily
associated
with
increased
friction
and
inflammation
due
to
pro‐inflammatory
M1‐type
macrophage
infiltration
in
the
articular
cavity.
Therefore,
strategies
simultaneously
increase
lubrication
relieve
remodel
damaged
microenvironment
are
of
great
significance
for
enhancing
its
treatment.
Herein,
multifunctional
core‐brush
nanoplatform
composed
ROS‐scavenging
polydopamine‐coated
SiO
2
core
lubrication‐enhancing
zwitterionic
poly(2‐methacryloyloxyethyl
phosphorylcholine)
(PMPC)
brush
loaded
anti‐inflammatory
drug
curcumin
by
reactive
oxygen
species
(ROS)‐liable
conjugation
(named
@PP‐Cur)
rationally
designed.
Benefiting
from
grafted
PMPC
brush,
tenacious
hydration
layer
enhanced
lubricity
reducing
abrasions
developed.
More
importantly,
based
on
mono‐iodoacetic
acid‐induced
arthritis
(MIA)
rat
model,
intra‐articular
injection
@PP‐Cur
can
effectively
alleviate
via
promoting
polarization
M1
M2
state
activating
nuclear
factor
erythroid
2‐related
(Nrf2)
signaling
pathway
attenuating
degradation
cartilage
matrix,
resulting
remodeling
into
pro‐regenerative
microenvironment.
As
result,
considerably
inhibit
OA
progression.
work
may
provide
novel
strategy
development
an
advanced
therapy.
Язык: Английский
Injectable microspheres filled with copper-containing bioactive glass improve articular cartilage healing by regulating inflammation and recruiting stem cells
Regenerative Biomaterials,
Год журнала:
2024,
Номер
12
Опубликована: Дек. 17, 2024
Abstract
Osteoarthritis
(OA)
is
a
frequent
chronic
illness
in
orthopedics
that
poses
major
hazard
to
patient
health.
In
situ
cell
therapy
emerging
as
therapeutic
option,
but
its
efficacy
influenced
by
both
the
inflammatory
milieu
and
amount
of
stem
cells,
limiting
use.
this
study,
we
designed
novel
injectable
porous
microsphere
(PM)
based
on
microfluidic
technology
can
support
mesenchymal
cells
(MSCs)
combining
polylactic–glycolic
acid
copolymer,
kartogenin,
polydopamine,
stromal
cell-derived
factor-1,
copper-doped
bioactive
glass
(CuBG).
The
ex
vivo
tests
demonstrated
PMs@CuBG
microspheres
were
biocompatible
facilitated
transformation
synovial
macrophages
from
pro-inflammatory
M1
anti-inflammatory
M2
phenotypes
releasing
CuBG
reduce
joint
inflammation.
At
same
time,
are
able
recruit
MSCs
into
cavity
encourage
their
differentiation
chondrocytes,
thereby
treating
articular
cartilage
injury.
rat
experimental
results
show
intra-articular
injection
rats
with
OA
improves
OARSI
scores,
aggrecan
content
ratio
col-2α-positive
indicating
reparative
effect
damaged
within
joint.
As
result,
predicted
provide
successful
approach
for
OA.
Язык: Английский