i-manager s Journal on Chemical Sciences,
Год журнала:
2024,
Номер
4(3), С. 22 - 22
Опубликована: Янв. 1, 2024
The
increasing
prevalence
of
neurodegenerative
diseases
such
as
Parkinson's
and
Alzheimer's
necessitate
more
advanced
neural
circuit
models
for
research
drug
development.
Traditional
rodent
fail
to
replicate
human
brain
complexity,
leading
high
late-stage
clinical
trial
failure
rates.
This
study
presents
the
development
optimisation
a
humanised
microfluidic
in
vitro
neuronal
model
using
compartmentalised
microfabricated
devices
culture
SH-SY5Y
neurons.
differentiation
protocol
was
refined
promote
long,
functional
neurons
suitable
forming
unidirectional
circuits.
Electrophysiological
assessment
through
calcium
imaging
multi-electrode
array
(MEA)
recordings
confirmed
activity.
successful
integration
into
PDMS
demonstrates
potential
this
long-term
studies
behaviour.
Further
developments
will
enhance
connectivity
disease
modelling
capabilities.
Pharmaceutics,
Год журнала:
2024,
Номер
16(10), С. 1314 - 1314
Опубликована: Окт. 10, 2024
The
limited
translatability
of
preclinical
experimental
findings
to
patients
remains
an
obstacle
for
successful
treatment
brain
diseases.
Relevant
models
elucidate
mechanisms
behind
pathogenesis,
including
cell-specific
contributions
and
cell-cell
interactions,
support
targeting
prediction
drug
responses
in
humans
are
urgently
needed,
given
the
species
differences
blood-brain
barrier
(BBB)
functions.
Human
microphysiological
systems
(MPS),
such
as
Organ-Chips,
emerging
a
promising
approach
address
these
challenges.
Here,
we
examined
advanced
Brain-Chip
that
recapitulates
aspects
human
cortical
parenchyma
BBB
one
model.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 10, 2024
Abstract
Compartmentalized
microfluidic
chips
play
an
important
role
in
understanding
the
cellular
mechanisms
involved
neurodegenerative
disorders.
Dorsal
root
ganglia
are
a
well-established
model
for
modelling
peripheral
nervous
system
(PNS),
but
their
development
on
chip
remains
limited.
Furthermore,
it
would
be
beneficial
devices
to
openable
order
access
biological
material
inside
analyses.
Easy
prototype
and
biocompatible,
styrenic
block
copolymers
(SBC)
alternative
polydimethylsiloxane
(PDMS)
that
offer
both
reversible
permanent
bonding
properties.
This
paper
presents
fast
straightforward
method
produce
compartmentalized
SBC
chips.
The
study
validates
culture
of
murine
dorsal
explants,
comparing
standard
methods,
obtain
PNS.
Moreover,
properties
permit
reuse
with
quick
easy
cleaning
protocol.
It
provides
direct
cells,
opening
way
imaging
molecular
biology
analysis.
comparison
resources
required
PDMS
highlights
importance
moving
reusable
devices.
These
detachable,
easy-to-manufacture
sustainable
all-thermoplastic
platforms
provide
prototyping
vitro
PNS
modeling.
i-manager s Journal on Chemical Sciences,
Год журнала:
2024,
Номер
4(3), С. 22 - 22
Опубликована: Янв. 1, 2024
The
increasing
prevalence
of
neurodegenerative
diseases
such
as
Parkinson's
and
Alzheimer's
necessitate
more
advanced
neural
circuit
models
for
research
drug
development.
Traditional
rodent
fail
to
replicate
human
brain
complexity,
leading
high
late-stage
clinical
trial
failure
rates.
This
study
presents
the
development
optimisation
a
humanised
microfluidic
in
vitro
neuronal
model
using
compartmentalised
microfabricated
devices
culture
SH-SY5Y
neurons.
differentiation
protocol
was
refined
promote
long,
functional
neurons
suitable
forming
unidirectional
circuits.
Electrophysiological
assessment
through
calcium
imaging
multi-electrode
array
(MEA)
recordings
confirmed
activity.
successful
integration
into
PDMS
demonstrates
potential
this
long-term
studies
behaviour.
Further
developments
will
enhance
connectivity
disease
modelling
capabilities.
