Abstract
Vitiligo
is
an
autoimmune
disease
characterized
by
epidermal
melanocyte
destruction,
with
abnormal
responses
and
excessive
oxidative
stress
as
two
cardinal
mechanisms.
Human
umbilical
mesenchymal
stem
cells‐derived
exosomes
(hUMSCs‐Exos)
are
regarded
promising
therapeutic
choice
for
diseases
due
to
potent
immunosuppressive
anti‐oxidative
properties,
which
can
be
potentiated
under
3D
cell
culture
condition.
Nevertheless,
whether
derived
from
spheroids
of
hUMSCs
(3D‐Exos)
exhibit
considerable
effect
on
vitiligo
the
underlying
mechanism
remain
elusive.
In
this
study,
systemic
administration
3D‐Exos
showed
a
remarkable
in
treating
mice
vitiligo,
revealed
ameliorated
skin
depigmentation,
less
CD8
+
T
cells
infiltration,
expanded
Treg
skin,
exerted
better
than
2D‐Exos.
Mechanistically,
prominently
facilitate
expansion
lesion
suppress
H
2
O
‐induced
melanocytes
apoptosis.
Forward
miRNA
profile
analysis
molecular
experiments
have
demonstrated
that
miR‐132‐3p
miR‐125b‐5p
enriched
greatly
contributed
these
biological
effects
targeting
Sirt1
Bak1
respectively.
aggregate,
efficiently
ameliorate
simultaneously
potentiating
cells‐mediated
immunosuppression
suppressing
stress‐induced
damage
via
delivery
miR‐125b‐5p.
The
employment
will
treament
vitiligo.
Journal of Cosmetic Dermatology,
Год журнала:
2025,
Номер
24(1)
Опубликована: Янв. 1, 2025
ABSTRACT
Background
Exosomes
are
extracellular
vesicles,
composed
of
a
phospholipid
bilayer,
that
primarily
derived
from
stem
cells.
The
contents
exosomes
can
be
incorporated
into
the
tissue
in
which
they
introduced,
presents
unique
therapeutic
option.
Aims
have
been
investigated
as
treatment
for
number
medical
ailments,
but
literature
supporting
these
indications
is
inconclusive.
In
addition,
much
study
on
and
their
uses
has
recently
completed.
Thus,
this
review
summarizes
efficacy
implications
different
dermatologic
conditions.
Methods
A
surrounding
use
multiple
dermatological
conditions
was
conducted.
Additionally,
we
present
numerous
practical
cases
patients
had
treated
with
exosomes.
Results
Overall,
success
treating
demonstrated
varying
literature,
preliminary
evidence
generally
positive.
patient
also
showed
satisfactory
clinical
outcomes
further
studies
will
necessary
to
fully
characterize
ideal
modalities
application,
including
formulation,
mode
distribution,
frequency
treatment.
Conclusions
may
serve
an
effective
option
wound
healing,
reconstruction
skin
flaps,
radiation
dermatitis,
acne
vulgaris,
psoriasis,
atopic
allergic
contact
lichen
simplex
chronicus,
vulvar
sclerosis,
systemic
lupus
erythematosus,
vitiligo
although
additional
needed
confirm
safety.
Chemical Communications,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Extracellular
vesicles
have
been
deemed
as
potential
drug
carriers
for
treatment
of
various
diseases.
Recent
advances
summarized,
including
the
sources,
delivery
function,
extraction
and
cargo-loading
technology
extracellular
vesicles.
Vitiligo
is
a
depigmentation
disease
caused
by
the
targeted
destruction
of
melanocytes,
resulting
in
skin
and
hair
significant
psychological
stress.
However,
mechanisms
underlying
its
onset
progression
remain
unclear.
Endoplasmic
reticulum
(ER)
stress,
which
linked
with
oxidative
stress
autoimmunity,
involved
development
vitiligo,
prolonged
ER
induces
apoptosis.
Sirtuin
1
(Sirt1)
might
be
key
regulator
Thus,
we
explored
how
Sirt1
modulates
stress-induced
melanocyte
apoptosis
vitro
vivo.
Our
results
showed
that
affected
when
upon
to
vitro.
inhibition
aggravated
vitiligo
phenotype
mice;
thereby
protecting
against
response,
abating
unfolded
protein
response.
These
suggest
impairment
could
accelerate
vitiligo.
Chinese Journal of Chromatography,
Год журнала:
2025,
Номер
43(5), С. 434 - 445
Опубликована: Май 1, 2025
Exosomes
are
nanoscale
vesicles
wrapped
in
lipid
bilayers
that
secreted
by
cells
and
carry
a
variety
of
proteins,
lipids,
nucleic
acids,
metabolites.
widely
present
various
bodily
fluids
mediate
intercellular
communication.
They
participate
physiological
pathological
processes,
including
immune
regulation,
angiogenesis,
tumorigenesis,
metastasis,
have
significant
clinical
diagnosis
treatment
potential.
source-rich,
structurally
stable,
reflect
the
states
their
parental
cells.
Therefore,
they
expected
to
serve
as
novel
diagnostic
markers
for
diseases.
In
addition,
stem-cell-derived
exosomes
show
therapeutic
potential
advantages
low
immunogenicity,
high
safety
easy
storage,
exhibit
neurodegenerative
disorder,
cardiovascular
disease,
cancer.
Furthermore,
highly
biocompatible,
natural
homing
properties,
capable
easily
penetrating
biological
barriers,
making
them
excellent
drug-delivery
carriers.
Isolation
enrichment
is
prerequisite
downstream
analysis
application.
High-purity,
high-yield,
high-throughput
exosome-isolation
methods
be
used
applications.
Based
on
physicochemical
properties
exosomes,
density,
size,
charge,
surface
composition,
mainly
divided
into
density-based
(e.g.,
differential
ultracentrifugation,
density-gradient
ultracentrifugation),
size-based
ultrafiltration,
size-exclusion
chromatography,
field-flow
fractionation),
polymer-precipitation
polyethylene-glycol-based
precipitation),
chemical
affinity
antibody-based,
aptamer-based,
surface-lipid-based
probes)
methods.
Currently,
basic
research
applications
face
number
challenges.
Firstly,
complexity
heterogeneity
lack
standardized
isolation
has
led
variable
results
hinder
comparing
reproducing
between
different
laboratories
settings.
Current
generally
hindered
insufficient
purity,
yield,
throughput,
difficulties
separating
specific
subpopulations,
which
seriously
restrict
development
exosome
field.
Secondly,
use
clinic,
few
technical
requirements,
efficient
inexpensive
lacking.
Commonly
classical
methods,
such
time-consuming,
labor-intensive,
require
large
sample
volumes,
inappropriate
Methods
immunoaffinity
can
isolate
from
precious
trace
samples
practice;
however,
costs,
recoveries,
operating
requirements
shortcomings
clinic.
robust
large-scale
preparing
There
an
urgent
need
develop
repeatable
scalable
batches
high-quality
owing
rapid
Generally,
progress
greatly
improve
our
understanding
functions
components
will
help
transform
effective
strategies
lead
new
precision-medicine
personalized-treatment
This
article
summarizes
latest
-enrichment
technologies
introduces
application
disease
markers,
agents,
drug
delivery
Finally,
future
developmental
trends
discussed.
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 11, 2024
Abstract
Vitiligo
is
a
depigmentation
disease
caused
by
the
targeted
destruction
of
melanocytes,
resulting
in
skin
and
hair
significant
psychological
stress.
However,
mechanisms
underlying
its
onset
progression
remain
unclear.
Endoplasmic
reticulum
(ER)
stress,
linked
with
oxidative
stress
autoimmunity,
involved
development
vitiligo,
prolonged
ER
induces
apoptosis.
Sirtuin
1
(Sirt1)
might
be
key
regulator
stress.Thus,
we
explored
how
Sirt1
modulates
stress-induced
melanocyte
apoptosis
vitro
vivo.
We
confirmed
that
affects
when
upon
to
vitro.
inhibition
aggravated
vitiligo
phenotype
mice;
thus,
protects
against
response,
abating
unfolded
protein
response.
These
results
suggest
impairment
could
accelerate
vitiligo.
