International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 138922 - 138922
Опубликована: Дек. 1, 2024
Язык: Английский
Advanced Science, Год журнала: 2024, Номер 11(38)
Опубликована: Авг. 5, 2024
Gene-editing technology has become a transformative tool for the precise manipulation of biological genomes and holds great significance in field animal disease-resistant breeding. Mastitis, prevalent disease husbandry, imposes substantial economic burden on global dairy industry. In this study, regulatory sequence gene editing breeding strategy successful creation gene-edited (GED) goats with enhanced mastitis resistance using ISDra2-TnpB system as model is proposed. This included targeted integration an innate inflammatory (IRS) into promoter region lysozyme (LYZ) gene. Upon Escherichia Coli (E. coli) mammary gland infection, GED exhibited increased LYZ expression, showing robust anti-mastitis capabilities, mitigating PANoptosis activation, alleviating blood-milk-barrier (BMB) damage. Notably, highly expressed only E. coli infection. study marks advent animals exogenous-free expression demonstrates feasibility gene-editing proposed study. addition, it provides novel blueprint developing strains, focusing specificity biosafety while providing research basis widespread application system.
Язык: Английский
Процитировано
6
Journal of Virology, Год журнала: 2024, Номер 99(1)
Опубликована: Дек. 23, 2024
The occurrence of viral diseases poses a huge threat and impact on human public health safety the development animal fishery industry. Here, strain single-chain antibody fragment, scFv-1, was isolated from phage display library construct by immunizing New Zealand white rabbits with rhabdovirus. In vitro analysis showed that could inhibit infection virus in multiple pathways, including adsorption, fusion, release. vivo revealed scFv-1 had preventive protective effect against virus. addition, we describe transposon-based transport neutralizing genes allows for long-term, continuous expression, avoiding need lifelong, repeated passive immunization treatment. sum, high-throughput screening neutralization based technology transposon vector-based gene transfer provides effective methods treating preventing repetitive immunotherapy. This study also reference prevention treatment unknown pathogens.IMPORTANCELivestock fisheries play an important role economic food security. frequent outbreaks have caused great losses to livestock industry, while increase drug resistance use antibiotics as well potential risks raised serious concerns. constructed purified rhabdovirus selected antibody, good activity, which validated found be able block phases thus role. reducing Our work not only but body long-lasting even permanent protection immunization.
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9316 - 9316
Опубликована: Авг. 28, 2024
Newborn lambs are susceptible to pathogenic bacterial infections leading enteritis, which affects their growth and development causes losses in sheep production. It has been reported that N6-methyladenosine (m6A) is closely related innate immunity, but the effect of m6A on small intestinal epithelial cells (IECs) mechanism involved have not elucidated. Here, we investigated effects lipopolysaccharide (LPS)-induced inflammatory responses, apoptosis oxidative stress primary IECs. First, extracted IECs were identified by immunofluorescence using cell signature protein cytokeratin 18 (
Язык: Английский
Процитировано
0
Cells, Год журнала: 2024, Номер 13(20), С. 1699 - 1699
Опубликована: Окт. 14, 2024
Mastitis is a common disease worldwide that affects the development of dairy industry due to its high incidence and complex etiology. Precise regulation cell death survival plays critical role in maintaining internal homeostasis, organ development, immune function organisms, regulatory abnormalities are mechanism various pathological changes. Recent research has shown regulated (RCD) crucial mastitis. The drugs treat can be widely used mastitis treatment important clinical significance. This paper will review molecular mechanisms apoptosis, autophagy, pyroptosis, ferroptosis, necroptosis their roles provide new perspective for targeted
Язык: Английский
Процитировано
0
Gene, Год журнала: 2024, Номер unknown, С. 149044 - 149044
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 138922 - 138922
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
0