Innovative Construction and Application of Bile Duct Organoids: Unraveling the Complexity of Bile Duct Diseases and Potential Therapeutic Strategies
Cancer Letters,
Год журнала:
2025,
Номер
unknown, С. 217619 - 217619
Опубликована: Март 1, 2025
Язык: Английский
Liver quad culture chip as a model for radiation injury research
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Апрель 11, 2025
Abstract
Both
cancer
patients
receiving
radiotherapy
and
civilians
in
a
mass
casualty
nuclear
event
may
suffer
from
radiation
induced
damage
to
organ
systems.
Radiation
liver
disease
(RILD)
can
cause
acute
long-term
dysfunction
that
potentially
leads
death.
The
objective
of
this
study
was
ascertain
the
validity
quad-culture
chip,
micro-physiological
system
comprising
primary
human
hepatocytes
non-parenchymal
cells
(NPCs),
including
sinusoidal
endothelial
cells,
hepatic
stellate
(HSCs),
Kupffer
as
model
for
RILD.
exposure
chip
resulted
DNA
cellular
senescence
NPCs.
We
observed
metabolic
dysfunction,
inflammation,
HSCs
activation.
Whole
genome
sequencing
revealed
gene
alterations
pathways
relevant
RILD,
well
potential
efficacy
N
-acetylcysteine
amide
(NACA)
against
NACA
exhibited
capacity
mitigate
decreased
impact
on
other
pathophysiological
changes.
CDKN1A
miR-34a-5p
were
validated
useful
response
treatment
biomarkers.
These
findings
highlight
an
effective
investigating
microenvironment
RILD
evaluating
therapeutic
countermeasures
Язык: Английский
Organoid Vascularization: Strategies and Applications
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 26, 2025
Abstract
Organoids
provide
3D
structures
that
replicate
native
tissues
in
biomedical
research.
The
development
of
vascular
networks
within
organoids
enables
oxygen
and
nutrient
delivery
while
facilitating
metabolic
waste
removal,
which
supports
organoid
growth
maturation.
Recent
studies
demonstrate
vascularized
models
offer
insights
into
tissue
interactions
promote
regeneration.
However,
the
current
limitations
establishing
functional
affect
growth,
viability,
clinical
translation
potential.
This
review
examines
organoids,
including
mechanisms
angiogenesis
vasculogenesis,
construction
strategies,
applications.
approaches
are
categorized
vivo
vitro
methods,
with
analysis
their
specific
advantages
limitations.
also
discusses
emerging
techniques
such
as
bioprinting
gene
editing
for
improving
vascularization
integration
organoid‐based
therapies.
Current
developments
indicate
potential
applications
modeling
human
diseases
developing
therapeutic
contributing
to
advances
translational
Язык: Английский