Small Extracellular Vesicles Orchestrate Cisplatin‐Induced Ototoxicity: Potential Biomarker and Targets Discovery DOI Creative Commons

Jingru Ai,

Shasha Zhang,

Mingchen Dai

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Май 24, 2025

Abstract Cisplatin‐induced ototoxicity remains a clinical dilemma with limited mechanistic understanding and no food drug administration (FDA)‐approved therapies. Despite emerging roles of small extracellular vesicles (sEV) in ototoxicity, their molecular cargo profiles causal to cisplatin‐induced are unexplored. This study systematically investigates sEV derived from cochlear explants treated cisplatin (Cis‐sEV) controls (Ctrl‐sEV) using multi‐omics profiling. Through RNA sequencing, 83 differentially expressed microRNAs (miRNAs) identified Cis‐sEV compared Ctrl‐sEV. Notably, mmu‐miR‐34a‐5p, mmu‐miR‐140‐5p, mmu‐miR‐15b‐5p, mmu‐miR‐25‐3p, mmu‐miR‐339‐5p significantly upregulation Cis‐sEVs. Predicted target pathways these miRNAs enriched apoptosis, inflammation, cellular damage, indicating potential involvement damage. LC‐MS/MS analysis reveals 90 upregulated 150 downregulated proteins Cis‐sEV, many involved damage response. Specifically, CLTC, CCT2, ANXA6, HSPA8 uniquely CLTC ANXA6 exclusively co‐localized hair cells (HCs) post‐cisplatin exposure, suggesting that originate primarily damaged HCs. Moreover, may serve as biomarker for verified both vitro vivo models. provides novel insights into the mechanisms identifies therapeutic targets.

Язык: Английский

Microfluidic Nanoparticle Separation for Precision Medicine DOI Creative Commons
Zhenwei Lan, Rui Chen, Da Zou

и другие.

Advanced Science, Год журнала: 2024, Номер unknown

Опубликована: Дек. 4, 2024

Abstract A deeper understanding of disease heterogeneity highlights the urgent need for precision medicine. Microfluidics, with its unique advantages, such as high adjustability, diverse material selection, low cost, processing efficiency, and minimal sample requirements, presents an ideal platform medicine applications. As nanoparticles, both biological origin therapeutic purposes, become increasingly important in medicine, microfluidic nanoparticle separation proves particularly advantageous handling valuable samples personalized This technology not only enhances detection, diagnosis, monitoring, treatment accuracy, but also reduces invasiveness medical procedures. review summarizes fundamentals techniques starting examination properties essential core principles that guide various methods. It then explores passive, active, hybrid techniques, detailing their principles, structures, Furthermore, contributions to advancements liquid biopsy nanomedicine. Finally, it addresses existing challenges envisions future development spurred by emerging technologies advanced materials science, 3D printing, artificial intelligence. These interdisciplinary collaborations are anticipated propel platformization significantly expanding potential

Язык: Английский

Процитировано

5

How Traditional Chinese Medicine Can Play a Role In Nanomedicine? A Comprehensive Review of the Literature DOI Creative Commons
Chi Wang,

K. J. Ren,

Mei Yang

и другие.

International Journal of Nanomedicine, Год журнала: 2025, Номер Volume 20, С. 6289 - 6315

Опубликована: Май 1, 2025

Traditional Chinese medicine (TCM), a time-honored practice rooted in natural therapeutics, has served as cornerstone safeguarding human health across millennia, aiding disease mitigation and life vitality preservation. However, many TCM active ingredients suffer from poor solubility, low bioavailability, uncertain toxicity weak targeting ability. Nanomedicine represents modern scientific frontier, emerging the precise engineering of unique nanoscale characteristics, with extensive applications encompassing targeted therapeutic delivery diverse biomedical fields. Although nanomedicine diverge fundamentally historical origins disciplinary foundations, growing investigations demonstrate their synergistic potential. In this review, nanosized been revealed an innovative strategy significant clinical value. Based on biological activities structural characteristics ingredients, we classify them into two categories: nanostructured formulations for nano-drug systems TCM. A systematic comprehensive analysis preparations specific functions to classes nanomedicines is highlighted. Insights advantage are also introduced. Subsequently, treatment, including anti-cancer, anti-inflammation anti-bacterial summarized. Finally, challenges future research directions emphasized, aiming offer guidance modernization nanomedicines.

Язык: Английский

Процитировано

0

Small Extracellular Vesicles Orchestrate Cisplatin‐Induced Ototoxicity: Potential Biomarker and Targets Discovery DOI Creative Commons

Jingru Ai,

Shasha Zhang,

Mingchen Dai

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Май 24, 2025

Abstract Cisplatin‐induced ototoxicity remains a clinical dilemma with limited mechanistic understanding and no food drug administration (FDA)‐approved therapies. Despite emerging roles of small extracellular vesicles (sEV) in ototoxicity, their molecular cargo profiles causal to cisplatin‐induced are unexplored. This study systematically investigates sEV derived from cochlear explants treated cisplatin (Cis‐sEV) controls (Ctrl‐sEV) using multi‐omics profiling. Through RNA sequencing, 83 differentially expressed microRNAs (miRNAs) identified Cis‐sEV compared Ctrl‐sEV. Notably, mmu‐miR‐34a‐5p, mmu‐miR‐140‐5p, mmu‐miR‐15b‐5p, mmu‐miR‐25‐3p, mmu‐miR‐339‐5p significantly upregulation Cis‐sEVs. Predicted target pathways these miRNAs enriched apoptosis, inflammation, cellular damage, indicating potential involvement damage. LC‐MS/MS analysis reveals 90 upregulated 150 downregulated proteins Cis‐sEV, many involved damage response. Specifically, CLTC, CCT2, ANXA6, HSPA8 uniquely CLTC ANXA6 exclusively co‐localized hair cells (HCs) post‐cisplatin exposure, suggesting that originate primarily damaged HCs. Moreover, may serve as biomarker for verified both vitro vivo models. provides novel insights into the mechanisms identifies therapeutic targets.

Язык: Английский

Процитировано

0